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11. 发明专利

JP2005189104A Method for condensing and separating protein or peptide 审中-公开
标题翻译：冷冻和分离蛋白质或肽的方法
公开(公告)号：JP2005189104A
公开(公告)日：2005-07-14
申请号：JP2003430898
申请日：2003-12-25
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： WATANABE MAKOTO , MATSUO HIDEKAZU , TODA CHIKAKO , NISHIMURA TADASHI
IPC分类号： G01N30/88 , B01J20/281 , C07K1/13 , C07K1/14 , C07K1/16 , C07K1/24 , G01N30/06 , G01N30/26 , G01N30/56 , G01N30/80 , G01N30/84
CPC分类号： C07K1/24
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting a carrier, having superior, selective capability of holding proteins or peptides to be condensed and separated, and selectively condensing and separating the proteins or the peptides through the use of the carrier.
SOLUTION: In the protein or peptide condensing and separating method, proteins or peptides containing amino acid residue, having a π-electron containing functional group, are separated through the use of a carrier having a π-electron containing functional group. The amino acid residue is preferably a tryptophan residue or a tryptophan modified with a sulfenyl compound, and the carrier is preferably a carrier having a phenyl group.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种检测载体的方法，具有优异的选择性的保持要冷凝和分离的蛋白质或肽的能力，并通过使用载体选择性地冷凝和分离蛋白质或肽。解决方案：在蛋白质或肽缩合和分离方法中，含有具有π电子的官能团的含氨基酸残基的蛋白质或肽通过使用具有π电子官能团的载体分离。氨基酸残基优选为用巯基化合物修饰的色氨酸残基或色氨酸，并且载体优选为具有苯基的载体。版权所有（C）2005，JPO＆NCIPI

12. 发明专利

JP2006105952A Method and kit for quantitative analysis of protein 有权
标题翻译：蛋白质定量分析的方法和工具包
公开(公告)号：JP2006105952A
公开(公告)日：2006-04-20
申请号：JP2004368510
申请日：2004-12-20
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： MATSUO HIDEKAZU , WATANABE MAKOTO , TODA CHIKAKO , NISHIMURA TADASHI
IPC分类号： G01N31/00 , C12Q1/37 , G01N27/62 , G01N27/64 , G01N30/00 , G01N30/88 , G01N33/68
CPC分类号： G01N33/6842
摘要： PROBLEM TO BE SOLVED: To provide a method for global quantitative analysis of protein that is applied effectively also for unpurified samples, such as biological samples, and achieves superior detection sensitivity and quantitativeness as compared with the conventional NBS method.
SOLUTION: A method for global quantitative analysis of protein comprising preparing two states of protein samples, a protein sample I for analysis and a control protein sample II; solubilizing the protein samples I and II by urea or guanidine hydrochloride; subjecting the solubilized protein samples I and II to modification using an NBSCl (heavy) reagent and NBSCl (light) reagent; mixing and desalting the modified protein samples I and II; resolubilizing by urea or guanidine hydrochloride; reducing and alkylating; subjecting to trypsin digestion, in the presence of urea or guanidine hydrochloride; separating the obtained peptide mixture using a media having a phenyl group; and subjecting the enriched modified peptide fragments to mass spectrometry, preferably using 3-CHCA, 3H4NBA or mixture of 3H4NBA and 4-CHCA as a matrix.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种用于全球定量分析蛋白质的方法，该方法也适用于未经纯化的样品如生物样品，并且与常规NBS方法相比具有优异的检测灵敏度和定量性。解决方案：一种用于蛋白质全球定量分析的方法，包括制备蛋白质样品的两种状态，用于分析的蛋白质样品I和对照蛋白质样品II; 通过尿素或盐酸胍溶解蛋白质样品I和II; 使用NBSCl（重）试剂和NBSCl（轻质）试剂对溶解的蛋白质样品I和II进行修饰; 混合和脱盐改性蛋白质样品I和II; 用尿素或盐酸胍重新溶解; 还原和烷基化; 在尿素或盐酸胍存在下进行胰蛋白酶消化; 使用具有苯基的介质分离得到的肽混合物; 并将富集的经修饰的肽片段进行质谱分析，优选使用3-CHCA，3H4NBA或3H4NBA和4-CHCA的混合物作为基质。版权所有（C）2006，JPO＆NCIPI

13. 发明专利

JP2005300430A Method for specifically detecting labeled peptide, and method for specifically detecting peak located to one side 审中-公开
标题翻译：用于特异性检测标记肽的方法和特异性检测位于一侧的峰的方法
公开(公告)号：JP2005300430A
公开(公告)日：2005-10-27
申请号：JP2004119580
申请日：2004-04-14
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： WATANABE MAKOTO , MATSUO HIDEKAZU , TODA CHIKAKO , NISHIMURA TADASHI
IPC分类号： G01N27/62 , G01N33/483 , G01N33/68
摘要： PROBLEM TO BE SOLVED: To provide a method capable of easily distinguishing a peak originating in labeled peptide (peptide-containing tryptophan) from a peak, originating in nonspecific adsorbed peptide (peptide which does not contain tryptophan), in a relative quantitative proteome analysis which uses an NBS reagent.
SOLUTION: Three sets about two kinds of samples to be compared with each other are considered as the combinations: (sample A + sample A), (sample B + sample B) and (sample A + sample B). One sample of each combination is labeled by using an unlabeled light reagent, the other is labeled by using a stable isotope labeled heavy reagent; and then, both the samples are mixed and analyzed by using a mass spectrometer.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种能够容易地区分源自标记肽（含肽色氨酸）的峰与源于非特异性吸附肽（不含色氨酸的肽）的峰，相对定量蛋白质组分析使用NBS试剂。（3）样品A（样品A），样品B +样品B和样品A +样品B的组合被认为是三组相互比较的两种样品。每个组合的一个样品通过使用未标记的轻试剂进行标记，另一个通过使用稳定同位素标记的重试剂进行标记; 然后，通过使用质谱仪将两个样品混合并分析。版权所有（C）2006，JPO＆NCIPI

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