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    • 12. 发明授权
    • Method for increasing the efficiency of recombinant AAV production
    • 提高重组AAV生产效率的方法
    • US6037177A
    • 2000-03-14
    • US908578
    • 1997-08-08
    • Richard Snyder
    • Richard Snyder
    • C12N15/09C12N5/10C12N15/864C12N15/35C12N15/63
    • C12N15/86C12N2750/14143
    • The present invention relates to methods and compositions for increasing the production of high titre stocks of recombinant AAV (rAAV) through regulation of expression of the AAV REP and CAP proteins. The methods and compositions of the invention are based on the observation that the low level expression of the AAV REP 78/68 protein increases the production of AAV viral capsid protein and efficiency of packaging resulting in production of higher titre recombinant viral stocks. The invention encompasses recombinant AAV vectors that direct the expression of AAV REP and CAP proteins and the use of such vectors for the production of novel stable cell lines capable of generating high titre rAAV vectors. The invention provides methods for regulating the expression of the AAV REP 78/68, REP 40/52 and CAP gene at the transcriptional and post-translational level. The methods and compositions of the invention can be used to produce high titre stocks of rAAV which can be used in gene therapy for the purpose of transferring genetic information into appropriate host cells for the management and correction of human diseases including inherited and acquired disorders.
    • 本发明涉及通过调节AAV REP和CAP蛋白的表达来增加重组AAV(rAAV)的高滴度储备的生产的方法和组合物。 本发明的方法和组合基于AAV REP 78/68蛋白质的低水平表达增加AAV病毒衣壳蛋白的产生和包装效率的观察结果,导致产生较高滴度的重组病毒种群。 本发明包括引导AAV REP和CAP蛋白的表达的重组AAV载体,以及这些载体用于产生能产生高滴度rAAV载体的新型稳定细胞系的用途。 本发明提供了在转录和翻译后水平调节AAV REP 78/68,REP 40/52和CAP基因表达的方法。 本发明的方法和组合物可用于生产高滴度的rAAV,其可用于基因治疗,以将遗传信息转移到合适的宿主细胞中,用于管理和矫正包括遗传和获得性疾病在内的人类疾病。
    • 15. 发明申请
    • Method of dynamically allocating usage of a shared resource
    • 动态分配共享资源的使用方法
    • US20050144281A1
    • 2005-06-30
    • US10734958
    • 2003-12-11
    • Richard Snyder
    • Richard Snyder
    • G06F9/50G06F15/173H04L12/24H04M3/523
    • G06F9/5066G06F2209/504H04L41/0896H04M3/5232H04M3/5237H04M2201/12Y02D10/22
    • A method of dynamically allocating usage of a shared resource between user A and user B includes the steps of establishing an initial allocation percentage for user B for using the shared resource and establishing a threshold allocation percentage for user B. The allocation percentage is modified for user B based on the availability of the shared resource to user A. If the shared resource is unavailable to user A, the allocation percentage for user B is decreased. If the shared resource is available to user A, the allocation percentage for user B is increased. Usage of the shared resource by user B is allocated in accordance with the modified allocation percentage provided that the modified allocation percentage is less than a pre-set threshold level. The method is particularly useful in dynamically allocating telephone calls into call centers.
    • 在用户A和用户B之间动态分配共享资源的使用的方法包括以下步骤:为用户B建立用于使用共享资源的初始分配百分比,并为用户B建立阈值分配百分比。为用户修改分配百分比 基于向用户A的共享资源的可用性。如果共享资源对于用户A不可用,则用户B的分配百分比减小。 如果共享资源对用户A可用,则用户B的分配百分比增加。 如果修改的分配百分比小于预先设定的阈值水平,则按照修改的分配百分比来分配用户B的共享资源的使用。 该方法在将电话动态分配到呼叫中心中特别有用。
    • 16. 发明授权
    • Filter assembly for cleaning cooling air for engines
    • 用于清洁发动机冷却空气的过滤器组件
    • US06022391A
    • 2000-02-08
    • US240210
    • 1999-01-29
    • Roy E. GreenleesRichard SnyderKenneth S. SchultzR. Ryan Greenlees
    • Roy E. GreenleesRichard SnyderKenneth S. SchultzR. Ryan Greenlees
    • F01P11/12F02B77/00
    • F01P11/12Y10S55/28
    • An engine filter assembly for cleaning cooling air for an internal-combustion engine for use in high-debris environment such as in roofing-removing machines includes a protective element housing attached to the machine at a remote position. The protective element housing houses a two-stage air filter having a cylindrical-shaped filter element with an outer pre-filter covering. An air inlet housing on the engine is connected to the protective element housing by a flexible coupling link. A shield protects the engine from direct contact with loose or air-born materials during the operation of the machine. A thermal sensing device reads the temperature of the engine and automatically shuts down the engine if the temperature elevates beyond specification, reducing the chance of engine failure.
    • 用于清洁用于高碎片环境的内燃机的冷却空气的发动机过滤器组件,例如在屋顶除尘机中包括在远程位置附接到机器的保护元件壳体。 保护元件壳体容纳具有带有外部预过滤器覆盖物的圆柱形过滤器元件的两级空气过滤器。 发动机上的空气入口壳体通过柔性联轴节连接到保护元件壳体。 屏蔽罩可保护发动机在机器运行过程中与松动或空气传播的材料直接接触。 如果温度升高超过规格,热感测装置读取发动机的温度并自动关闭发动机,减少发动机故障的机会。