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11. 发明专利

AU2003272419A1 RAPIDLY DEGRADED REPORTER FUSION PROTEINS 未知
公开(公告)号：AU2003272419A1
公开(公告)日：2004-04-30
申请号：AU2003272419
申请日：2003-09-16
申请人： ZDANOVSKY ALEXEY , ZDANOVSKAIA MARINA , MA DONGPING , WOOD KEITH V , ALMOND BRIAN D , WOOD MONIKA G , PROMEGA CORP
发明人： ZDANOVSKAIA MARINA , MA DONGPING , WOOD KEITH V , ALMOND BRIAN D , WOOD MONIKA G , ZDANOVSKY ALEXEY
IPC分类号： C12N15/09 , C07H21/04 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/06 , C12N9/10 , C12N9/22 , C12N9/24 , C12N9/38 , C12P21/04 , C12Q1/02 , C12Q1/68
摘要： A fusion polypeptide comprising a protein of interest which has a reduced half-life of expression, and a nucleic acid molecule encoding the fusion polypeptide, are provided.

12. 发明专利

JP2007006910A Synthetic nucleic acid molecule compositions and methods of preparation 审中-公开
标题翻译：合成核酸分子组合物及其制备方法
公开(公告)号：JP2007006910A
公开(公告)日：2007-01-18
申请号：JP2006288147
申请日：2006-10-23
申请人： Promega Corp , プロメガ・コーポレーション
发明人： WOOD KEITH V , WOOD MONIKA G , ZHUANG YAO , PAGUIO AILEEN
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/67
CPC分类号： C12N9/0069 , C12N15/67
摘要： PROBLEM TO BE SOLVED: To provide synthetic nucleic acid molecules having altered codon usage without introduction of inappropriate or unintended transcription regulatory sequence for expression in a particular host cell. SOLUTION: A synthetic nucleic acid molecule is provided which comprises at least 300 nucleotides of a coding region for a polypeptide, has a codon composition differing at more than 25% of the codons from a wild type nucleic acid sequence encoding a polypeptide, and has at least 3-fold fewer transcription regulatory sequences than the average number of sequence which would result if the differing codons were randomly selected, wherein the transcriptional regulatory sequence is selected from the group consisting of transcription factor binding sequences, intron splice sites, poly (A) addition sites and promoter sequences, and wherein the polypeptide encoded by the synthetic nucleic acid molecule has at least 85% of sequence identity to the polypeptide encoded by the wild type nucleic acid sequence. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供具有改变的密码子使用的合成核酸分子，而不引入用于在特定宿主细胞中表达的不适当或非预期的转录调节序列。解决方案：提供包含多肽编码区的至少300个核苷酸的合成核酸分子，其密码子组成不同于编码多肽的野生型核酸序列的密码子的25％以上，并且具有比如果随机选择不同密码子时将导致的平均序列数少至少3倍的转录调节序列，其中转录调控序列选自转录因子结合序列，内含子剪接位点，聚（A）加成位点和启动子序列，并且其中由合成核酸分子编码的多肽与野生型核酸序列编码的多肽具有至少85％的序列同一性。版权所有（C）2007，JPO＆INPIT

13. 发明专利

JP2013208136A Mutant dehalogenase with increased binding stability 审中-公开
标题翻译：具有增加的结合稳定性的突变体
公开(公告)号：JP2013208136A
公开(公告)日：2013-10-10
申请号：JP2013131105
申请日：2013-06-21
申请人： Promega Corp , プロメガコーポレイションPromega Corporation
发明人： DARZINS ALDIS , ENCELL LANCE P , JOHNSON TONNY , KLAUBERT DIETER , LOS GEORGYI V , MCDOUGALL MARK , WOOD KEITH V , WOOD MONIKA G , ZIMBRICH CHAD
IPC分类号： C12N15/09 , C07K14/47 , C12N9/04 , C12N9/14 , C12Q1/26
CPC分类号： C12N9/14 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07K2299/00 , C12N9/0069 , C12Q1/26 , C12Q1/34 , C12Q1/66 , C12Y113/12005 , C12Y308/01005
摘要： PROBLEM TO BE SOLVED: To provide mutant proteins covalently linked (tethered) to one or more functional groups and methods for their use, and a mutant dehalogenase optionally fused to a protein of interest.SOLUTION: One or more functional groups are tethered (linked) to a protein to a fusion protein (chimera) which includes a protein via a covalent bond or otherwise stable bond. The mutant dehalogenase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) dehalogenase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has two amino acid substitutions relative to the wild-type dehalogenase.
摘要翻译：要解决的问题：提供共价连接（连接）到一个或多个官能团的突变蛋白及其使用方法，以及任选地与感兴趣的蛋白质融合的突变体脱卤酶。解决方案：将一个或多个官能团连接（连接）涉及包含通过共价键或其他稳定键的蛋白质的融合蛋白（嵌合体）的蛋白质。突变体脱卤酶能够与相应的非突变（野生型）脱卤素酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有两个氨基酸取代，型脱卤素酶。

14. 发明专利

JP2012090635A Permuted and nonpermuted luciferase biosensor 审中-公开
标题翻译：未经授权的和未经授权的LUCIFERASE生物传感器
公开(公告)号：JP2012090635A
公开(公告)日：2012-05-17
申请号：JP2011269846
申请日：2011-12-09
申请人： Promega Corp , プロメガコーポレイションPromega Corporation
发明人： BINKOWSKI BROCK , FAN FRANK , WIGDAL SUSAN , WOOD KEITH V , WOOD MONIKA G
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12Q1/02 , C12Q1/26 , G01N21/76
CPC分类号： C12N9/0069 , C12N9/0004 , C12Q1/66 , C12Y113/12007 , G01N33/84
摘要： PROBLEM TO BE SOLVED: To provide a modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases.SOLUTION: There are provided a circularly permuted anthozoan luciferase protein and a circularly permuted decapod crustacean luciferase protein, containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further, a modified anthozoan luciferase protein and a modified decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest are provided.
摘要翻译：要解决的问题：提供修饰的荧光素酶蛋白，其是包括cAMP，cGMP，钙，其螯合剂，激酶或磷酸酶的分子的传感器。提供了一种循环置换的anthozoan萤光素酶蛋白质和循环置换的十足甲壳类甲壳素荧光素酶蛋白，其含有一个或多个异源氨基酸序列，其中至少一个直接或间接与感兴趣的分子相互作用。此外，提供了一种修饰的抗虫虫萤光素酶蛋白质和修饰的十足甲壳蛋白萤光素酶蛋白质，其含有一个或多个异源氨基酸序列的插入，其中至少一种与感兴趣的分子直接或间接相互作用。版权所有（C）2012，JPO＆INPIT

15. 发明专利

JP2013078329A Mutant hydrolase protein with enhanced kinetics and functional expression 有权
标题翻译：具有增强动力学和功能表达的突变型水解蛋白
公开(公告)号：JP2013078329A
公开(公告)日：2013-05-02
申请号：JP2012264713
申请日：2012-12-03
申请人： Promega Corp , プロメガコーポレイションPromega Corporation
发明人： DARZINS ALDIS , ENCELL LANCE P , OHANA RACHEL FRIEDMAN , OTTO PAUL , VIDUGIRIS GEDIMINAS , WOOD KEITH V , WOOD MONIKA G , ZIMMERMAN KRIS , SLATER MICHAEL R , ZHAO KATE QIN
IPC分类号： C12N15/09 , C07K19/00 , C12N1/21 , C12N5/10 , C12N9/14 , C12P21/02 , C12Q1/02 , C12Q1/34
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： PROBLEM TO BE SOLVED: To provide a mutant dehalogenase that forms a bond with a dehalogenase substrate, which is more stable than the bond formed between a corresponding wild-type dehalogenase and the substrate, and has enhanced kinetics and functional expression.SOLUTION: The mutant dehalogenase includes at least one amino acid substitution to improve the functional expression or binding kinetics at a position 58, 78, 155, 172, 224, 291 and 292 in a sequence of the corresponding wild-type dehalogenase.
摘要翻译：要解决的问题：提供与脱卤酶底物形成键的突变型脱卤酶，其比相应的野生型脱卤素酶和底物之间形成的键更稳定，并且具有增强的动力学和功能性表达。解决方案：突变体脱卤酶包括至少一个氨基酸取代，以改善相应野生型脱卤素酶序列中58,78,155,172,224,291和292位的功能表达或结合动力学。版权所有（C）2013，JPO＆INPIT

16. 发明专利

JP2013051968A Permuted and nonpermuted luciferase biosensor 有权
标题翻译：未经授权的和未经授权的LUCIFERASE生物传感器
公开(公告)号：JP2013051968A
公开(公告)日：2013-03-21
申请号：JP2012248580
申请日：2012-11-12
申请人： Promega Corp , プロメガコーポレイションPromega Corporation
发明人： BINKOWSKI BROCK , FAN FRANK , WIGDAL SUSAN , WOOD KEITH V , WOOD MONIKA G
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12Q1/26 , C12Q1/68 , G01N33/50
CPC分类号： C12N9/0069 , C12N9/0004 , C12Q1/66 , C12Y113/12007 , G01N33/84
摘要： PROBLEM TO BE SOLVED: To provide a modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases or phosphatases.SOLUTION: A circularly permuted anthozoan luciferase protein and a modified luciferase protein of circularly permuted decapod crustacean, for example, are provided which contain one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. At least one of which contains an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.
摘要翻译：要解决的问题：提供修饰的萤光素酶蛋白，其是用于包括cAMP，cGMP，钙，其螯合剂，激酶或磷酸酶的分子的传感器。解决方案：提供了一种循环置换的载体荧光素酶蛋白和循环置换的十足甲壳类甲壳动物的修饰的荧光素酶蛋白，其含有一个或多个异源氨基酸序列，其中至少一个与其分子中的至少一个直接或间接相互作用利益。其中至少一个含有一个或多个异源氨基酸序列的插入，其中至少一个直接或间接与感兴趣的分子相互作用。版权所有（C）2013，JPO＆INPIT

17. 发明专利

JP2012044995A Covalent tethering of functional group to protein and substrate therefor 有权
标题翻译：功能组与蛋白质及其底物的共价合成
公开(公告)号：JP2012044995A
公开(公告)日：2012-03-08
申请号：JP2011220845
申请日：2011-10-05
申请人： Promega Corp , プロメガコーポレイションPromega Corporation
发明人： DARZINS ALDIS , ENCELL LANCE P , JOHNSON TONNY , KLAUBERT DIETER , LOS GEORGYI V , MCDOUGALL MARK , WOOD KEITH V , WOOD MONIKA G , ZIMBRICH CHAD
IPC分类号： C12N15/09 , C07K1/13 , C07K1/22 , C12N1/02 , C12N9/14 , C12N11/00 , C12N11/14 , C12Q1/02 , C12Q1/34 , C12Q1/37 , C12Q1/48 , C12Q1/66 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/536 , G01N33/543 , G01N33/553
CPC分类号： C12Q1/34 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C09B1/00 , C09B11/24 , C09B23/04 , C09B23/06
摘要： PROBLEM TO BE SOLVED: To provide a mutant protein which is covalently linked (tethered) to a single or a plurality of functional groups or a method of using thereof, and a mutant hydrolase optionally fused to a protein of interest.SOLUTION: There is provided the method for tethering (linking), e.g., via a covalent or otherwise stable bond, one or more functional groups to the protein or to a fusion protein (chimera) which includes the protein. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. The mutant hydrolase includes two amino acid substitutions relative to the wild-type hydrolase.
摘要翻译：待解决的问题：提供共价连接（连接）到单个或多个官能团的突变蛋白或其使用方法，以及任选地与感兴趣的蛋白质融合的突变型水解酶。提供了例如通过共价或其它稳定键将蛋白质或包含蛋白质的融合蛋白（嵌合体）一个或多个官能团连接（连接）的方法。突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定。突变体水解酶包括相对于野生型水解酶的两个氨基酸取代。版权所有（C）2012，JPO＆INPIT

18. 发明专利

JP2010081942A Synthetic nucleic acid molecule composition and method of preparation 审中-公开
标题翻译：合成核酸分子组合物及其制备方法
公开(公告)号：JP2010081942A
公开(公告)日：2010-04-15
申请号：JP2010008451
申请日：2010-01-18
申请人： Promega Corp , プロメガ・コーポレーション
发明人： WOOD KEITH V , WOOD MONIKA G , ZHUANG YAO , PAGUIO AILEEN
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/67
CPC分类号： C12N9/0069 , C12N15/67
摘要： PROBLEM TO BE SOLVED: To provide synthetic nucleic acid molecules having altered codon usage without introduction of inappropriate or unintended transcription regulatory sequence for expression in a particular host cell. SOLUTION: There is provided the synthetic nucleic acid molecule comprising at least 300 nucleotides of a coding region for a polypeptide, having a codon composition differing at more than 25% of the codons from a wild type nucleic acid sequence encoding a polypeptide, and having at least 3-fold fewer transcription regulatory sequences than the average number of sequence which would result if the differing codons were randomly selected, wherein the transcriptional regulatory sequence is selected from the group consisting of transcription factor binding sequences, intron splice sites, poly (A) addition sites and promoter sequences, and wherein the polypeptide encoded by the synthetic nucleic acid molecule has at least 85% of sequence identity to the polypeptide encoded by the wild type nucleic acid sequence. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供具有改变的密码子使用的合成核酸分子，而不引入用于在特定宿主细胞中表达的不适当或非预期的转录调节序列。解决方案：提供了包含多肽编码区的至少300个核苷酸的合成核酸分子，其密码子组成不同于编码多肽的野生型核酸序列的密码子的25％以上，并且具有比如果随机选择不同密码子时将导致的平均序列数少至少3倍的转录调节序列，其中转录调节序列选自转录因子结合序列，内含子剪接位点，聚（A）加成位点和启动子序列，并且其中由合成核酸分子编码的多肽与野生型核酸序列编码的多肽具有至少85％的序列同一性。版权所有（C）2010，JPO＆INPIT

19. 发明公开

EP1558729A4 RAPIDLY DEGRADED REPORTER FUSION PROTEINS 有权
标题翻译： FAST开采报导融合蛋白
公开(公告)号：EP1558729A4
公开(公告)日：2006-09-06
申请号：EP03754599
申请日：2003-09-16
申请人： PROMEGA CORP
发明人： ZDANOVSKY ALEXEY , ZDANOVSKAIA MARINA , MA DONGPING , WOOD KEITH V , ALMOND BRIAN D , WOOD MONIKA G
IPC分类号： C12N15/09 , C07H21/04 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/06 , C12N9/10 , C12N9/22 , C12N9/24 , C12N9/38 , C12P21/04 , C12Q1/02 , C12Q1/68 , C12N9/14 , A61K38/44 , C07K1/00 , C12N1/20 , C12N9/62 , C12N15/00 , C12P21/06 , C12Q1/00 , C12Q1/34
CPC分类号： C12N15/67 , C07K2319/60 , C07K2319/61 , C07K2319/95 , C12N15/63 , C12Q1/6876 , C12Q1/6897

20. 发明公开

EP1654359A4 SYNTHETIC NUCLEIC ACIDS FROM AQUATIC SPECIES 审中-公开
标题翻译：水生物种的合成核酸
公开(公告)号：EP1654359A4
公开(公告)日：2006-11-22
申请号：EP03819255
申请日：2003-11-20
申请人： PROMEGA CORP
发明人： ALMOND BRIAN D , WOOD MONIKA G , WOOD KEITH V
IPC分类号： C12N15/09 , C12N15/10 , C07H21/04 , C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/00 , C12N15/12 , C12N15/67 , C12N15/85
CPC分类号： C12N9/0069 , C07K14/43595 , C12N15/67 , C12N15/8212 , C12N15/8216

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