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    • 13. 发明申请
    • Method of Analyzing Protein Structure Affinity Relationship
    • 分析蛋白质结构亲和关系的方法
    • US20080057592A1
    • 2008-03-06
    • US11571337
    • 2005-07-04
    • Yoshiya Oda
    • Yoshiya Oda
    • G01N33/00
    • G01N33/6803B01J20/286B01J20/3244B01J2220/54G01N33/6848G01N2500/02Y10T436/24
    • A method for analyzing a structural affinity relationship between plural kinds of proteins and a compound, comprising the steps of (a) using a compound-immobilized carrier to purify plural kinds of proteins bound to the compound on the carrier, from a group of isotope-labeled proteins; (b) using a compound-immobilized carrier to purify plural kinds of proteins bound to the compound on the carrier, from a group of proteins brought into contact with a compound beforehand; (c) mixing the proteins obtained in step (a) and step (b); (d) analyzing the mixture obtained in step (c) with mass spectrometry; (e) identifying each of plural kinds of proteins based on information obtained by the mass spectrometry; and (f) obtaining an intensity ratio between a labeled peak and a non-labeled peak of each protein, thereby quantitating an affinity ratio of the compound to each protein.
    • 一种用于分析多种蛋白质和化合物之间的结构亲和关系的方法,包括以下步骤:(a)使用固定化合物的载体从载体上与所述化合物结合的多种蛋白质从一组​​同位素 - 标记蛋白; (b)使用化合物固定化载体从预先与化合物接触的一组蛋白质中纯化与载体上的化合物结合的多种蛋白质; (c)混合步骤(a)和步骤(b)中获得的蛋白质; (d)用质谱分析步骤(c)中得到的混合物; (e)基于通过质谱法获得的信息来鉴定多种蛋白质中的每一种; 和(f)获得每种蛋白质的标记峰和非标记峰之间的强度比,从而定量化合物与每种蛋白质的亲和比。