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    • 18. 发明公开
    • PROCESS FOR PREPARATION OF SUGARS AND SYRUPS
    • 制备糖和糖浆的方法
    • EP2984173A1
    • 2016-02-17
    • EP14783224.0
    • 2014-04-10
    • Novozymes A/S
    • ELDER, MichaelDEINHAMMER, RandallCUI, Xiaoyuan
    • C12P19/00C12N9/26
    • C12P19/02C12P19/14C12P19/16C12P19/20C12P19/24C12P2201/00C13K1/06C13K11/00
    • The present invention relates to a process for producing a syrup comprising liquefying an aqueous granular starch slurry with an alpha-amylase variant comprising an alteration at one or more positions corresponding to any of positions 1, 2, 68, 71, 126, 133, 142, 144, 156, 158, 176, 185, 201, 205, 213, 239, 279, 316, 318, 360, 416, 437 and 450 of SEQ ID NO: 1 to provide liquefied starch-containing material; saccharifying the liquefied starch-containing material in the presence of a glucoamylase, and a pullulanase derived from Bacillus deramificans, Bacillus subtilis, Bacillus amyloderamicans, or Bacillus acidopullulyticus to provide a dextrose syrup, and optionally isomerizing to provide a fructose syrup.
    • 本发明涉及生产糖浆的方法,其包括用α-淀粉酶变体液化含水颗粒淀粉浆料,所述α-淀粉酶变体在对应于位置1,2,68,71,126,133,142中的任一个的一个或多个位置处具有改变 提供液化的含淀粉材料;以及提供液化的含淀粉材料,所述液化的含淀粉材料包含选自SEQ ID NO:1,SEQ ID NO:1的144,156,158,176,185,201,205,213,239,279,316,318,360,416,437和450的化合物, 在葡糖淀粉酶和衍生自芽孢杆菌(Bacillus deramificans),枯草芽孢杆菌,淀粉芽孢杆菌(Bacillus amyloderamificans)或嗜酸乳杆菌(Bacillus acidopullulyticus)的支链淀粉酶存在下糖化含葡萄糖淀粉的材料以提供葡萄糖糖浆,并任选地异构化以提供果糖糖浆。