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12. 发明申请

US20110274667A1 METHODS FOR OBTAINING CELL POPULATIONS FROM ADIPOSE TISSUE 审中-公开
标题翻译：从ADIPOSE组织获得细胞群体的方法
公开(公告)号：US20110274667A1
公开(公告)日：2011-11-10
申请号：US13124632
申请日：2009-10-19
申请人： Anthony Donofrio , Delara Motlagh , David L. Amrani , Amy Cohen
发明人： Anthony Donofrio , Delara Motlagh , David L. Amrani , Amy Cohen
IPC分类号： A61K35/12 , A61P9/10 , A61P13/12 , A61P37/06 , A61P27/02 , A61P17/02 , C12N5/077 , A61P3/10
CPC分类号： C12N5/0653 , C12N2509/00
摘要： Provided herein are methods of efficiently obtaining large numbers of viable, freshly isolated cells from small amounts of adipose tissue, as well as methods of enriching or selecting for target cell populations found therein. In certain embodiments, the method of obtaining a population of cells from adipose tissue comprises incubating the adipose tissue in a solution comprising an enzyme at a concentration which is at least 200 U/ml solution and not more than about 319 U/ml solution. In some embodiments, the method is devoid of any steps which expand the population of cells obtained. In certain aspects, the method further comprises positive or negative selection steps for obtaining an enriched population of target cells from adipose tissue. Related methods of preparing a pharmaceutical composition comprising cells for administration to a patient and methods of treating a disease or medical condition in a patient are further provided herein.
摘要翻译：本文提供了从少量脂肪组织有效地获得大量可行的，新鲜分离的细胞的方法，以及对其中发现的靶细胞群进行富集或选择的方法。在某些实施方案中，从脂肪组织获得细胞群的方法包括将脂肪组织在包含浓度为至少200U / ml且不超过约319U / ml溶液的酶的溶液中孵育。在一些实施方案中，该方法没有扩大获得的细胞群体的任何步骤。在某些方面，该方法还包括用于从脂肪组织获得富集的靶细胞群的阳性或阴性选择步骤。本文还提供制备包含用于给患者的细胞的药物组合物的相关方法和治疗患者的疾病或医学病症的方法。

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