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    • 11. 发明申请
    • Fermentative Production of Organic Compounds Using Substances Containing Dextrin
    • 使用含有糊精的物质发酵生产有机化合物
    • US20080318287A1
    • 2008-12-25
    • US12094646
    • 2006-11-27
    • Matthias BoyStephan Freyer
    • Matthias BoyStephan Freyer
    • C12P13/08
    • C12P7/26C12P7/42C12P7/62C12P7/6418C12P7/6427C12P7/6463C12P13/00C12P13/005C12P13/04C12P19/14C12P19/30C12P19/38
    • A process for the production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least 1N atom by means of fermentation, comprises the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and liquefying the millbase present in the aqueous liquid in the presence of at least one starch-liquefying enzyme, obtaining an aqueous dextrin-containing medium (1) which comprises at least a part of the nonstarchy solid constituents of the starch feedstock; and b) using the aqueous dextrin-containing medium (1) in a fermentation for culturing a microorganism which is capable of overproducing the organic compound; enzymes which hydrolyze the dextrins to monosaccharides being added in an amount of less than 0.001% by weight based on the total weight of the starch feedstock employed, or not at all.
    • 通过发酵生产具有至少3个C原子或具有至少2个C原子和至少1N原子的至少一种有机化合物的方法包括以下步骤:a1)研磨淀粉原料,从而得到碾磨基 其包括淀粉原料的至少部分非淀粉样固体成分; a2)在至少一种淀粉 - 液化酶的存在下,将磨粒悬浮在水性液体中并液化存在于水性液体中的研磨液,得到含有糊精的培养基(1),其含有至少一部分非淀粉样固体 淀粉原料成分; 和b)在含有糊精的培养基(1)中用于培养能够过量产生有机化合物的微生物的发酵中; 基于所使用的淀粉原料的总重量,或者根本不加入将糊精水解成单糖的酶的加入量小于0.001重量%。
    • 14. 发明授权
    • Fermentative production of organic compounds using substances containing dextrin
    • 使用含有糊精的物质发酵生产有机化合物
    • US08728773B2
    • 2014-05-20
    • US12094646
    • 2006-11-27
    • Matthias BoyStephan Freyer
    • Matthias BoyStephan Freyer
    • C12P13/08C12P13/04C12P7/26
    • C12P7/26C12P7/42C12P7/62C12P7/6418C12P7/6427C12P7/6463C12P13/00C12P13/005C12P13/04C12P19/14C12P19/30C12P19/38
    • Process for the production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least 1 N atom by fermentation. In particular, the process comprises milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; suspending the millbase in an aqueous liquid and liquefying the millbase present in the aqueous liquid in the presence of at least one starch-liquefying enzyme, obtaining an aqueous dextrin-containing medium (1) which comprises at least a part of the nonstarchy solid constituents of the starch feedstock; and using the aqueous dextrin-containing medium (1) in a fermentation for culturing a microorganism which is capable of overproducing the organic compound; wherein enzymes which hydrolyze the dextrins to monosaccharides are being added in an amount of less than 0.001% by weight based on the total weight of the starch feedstock employed, or not at all.
    • 通过发酵生产具有至少3个C原子或具有至少2个C原子和至少1个N原子的至少一种有机化合物的方法。 特别地,该方法包括研磨淀粉原料,从而获得包含至少部分淀粉原料的非淀粉状固体成分的研磨料; 在至少一种淀粉 - 液化酶的存在下使磨浆液悬浮在水性液体中并液化存在于水性液体中的研磨液,得到含水性糊精的培养基(1),其含有至少一部分非淀粉样固体成分 淀粉原料; 以及使用含水糊精的培养基(1)进行发酵,培养能够过量生成有机化合物的微生物; 其中将糊精水解成单糖的酶的加入量相对于所用淀粉原料的总重量小于0.001重量%,或根本不加入。
    • 15. 发明申请
    • Fermentative Production of Organic Compounds
    • 有机化合物的发酵生产
    • US20080254515A1
    • 2008-10-16
    • US12095130
    • 2006-11-28
    • Matthias BoyStephan Freyer
    • Matthias BoyStephan Freyer
    • C12P21/04C12P13/08
    • C12P19/14C12P13/08C12P13/14
    • The invention relates to a method for producing at least one organic compound comprising at least 3 C-atoms or at least 2 C-atoms and at least 1 N-atom by fermentation. Said method comprises the following steps: i) a starch source is ground in order to obtain a grinding material which contains at least one part of the non-starch containing solid components of the starch source; ii) the grinding material is suspended in an aqueous liquid in an amount such that the dry mass content in the suspension is at least 45 wt. %, iii) the starch component is hydrolysed in the grinding material by liquefying and optionally, subsequently, sweetening, in order to obtain an aqueous medium M, which contains the hydrolysed starch components and at least one part of the non-starch containing solid components of the starch source, is obtained; and iv) the aqueous substance M obtained in step iii) is used for fermentation in order to cultivate a micro-organism which is capable of over-producing the organic compound; the suspension obtained in step ii) is heated in step iii) by introducing vapour into the suspension, in order to obtain temperatures greater than the pasting temperature of the starch obtained in the grinding materials.
    • 本发明涉及通过发酵生产至少一种含有至少3个C原子,至少2个C原子和至少1个N原子的有机化合物的方法。 所述方法包括以下步骤:i)研磨淀粉源以获得含有至少一部分淀粉源的非淀粉固体组分的研磨材料; ii)研磨材料悬浮在水性液体中,其量使得悬浮液中的干质量含量至少为45重量%。 %,iii)淀粉组分在研磨材料中通过液化和任选地随后进行甜化而水解,以获得含有水解淀粉组分的水性介质M和至少一部分含淀粉的固体组分 的淀粉来源; 和iv)将步骤iii)中获得的水性物质M用于发酵,以培养能够过度生产有机化合物的微生物; 将步骤ii)中获得的悬浮液在步骤iii)中通过将蒸气引入悬浮液中来加热,以获得大于在研磨材料中获得的淀粉的糊化温度的温度。