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    • 12. 发明申请
    • Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
    • 提高位点特异性多核苷酸交换效率的方法和组合
    • US20070143881A1
    • 2007-06-21
    • US11427947
    • 2006-06-30
    • Dennis L. BidneySheila E. MaddockXinli E. Wu
    • Dennis L. BidneySheila E. MaddockXinli E. Wu
    • A01H1/00
    • C12N15/8213C12N15/8205C12N15/8207
    • Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.
    • 使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。 所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中,将包含位点特异性重组系统的至少一个组分的组合物注入胚囊,提供更好的表达效率 ,重组,整合,交换,切除和/或转化感兴趣的多核苷酸。 感兴趣的多核苷酸可以稳定地整合到卵细胞,合子,胚或胚乳以及由其产生的组织,植物部分和/或植物的基因组中。 还提供了通过方法产生并包含感兴趣的多核苷酸的细胞,卵细胞,合子,胚胎,胚乳,组织,种子和/或植物。
    • 15. 发明授权
    • Method of plant transformation
    • 植物转化方法
    • US06265638B1
    • 2001-07-24
    • US09407574
    • 1999-09-28
    • Dennis L. BidneyChristopher Jay Scelonge
    • Dennis L. BidneyChristopher Jay Scelonge
    • C12N1582
    • C12N15/8241C12N15/8205C12N15/8209
    • Compositions and methods for the efficient co-transformation of a plant are provided. Novel compositions are Agrobacterium strains that have been engineered to comprise at least two binary vector plasmids in addition to a helper plasmid comprising the vir functions. Each of the binary vectors comprises its own T-DNA borders flanking a heterologous nucleotide sequence of interest. Methods of the invention comprise the use of these novel multiple-binary vector Agrobacterium strains to co-transform a plant. In this manner, heterologous nucleotide sequences of interest residing on different binary vectors can be independently introduced into the plant in a single transformation event and incorporated in the plant's nuclear DNA in an unlinked manner. The invention also provides for regenerated, fertile transgenic plants, transgenic seeds produced therefrom, and T1 and subsequent generations.
    • 提供了植物有效共同转化的组合物和方法。 除了包含vir功能的辅助质粒之外,新型组合物是经过工程改造以包含至少两种二元载体质粒的农杆菌菌株。 每个二元载体包含在感兴趣的异源核苷酸序列侧翼的其自身的T-DNA边界。 本发明的方法包括使用这些新型多元二元载体农杆菌菌株共同转化植物。 以这种方式,驻留在不同二元载体上的感兴趣的异源核苷酸序列可以在单个转化事件中独立地引入植物中,并以非连接的方式并入植物的核DNA中。 本发明还提供了再生的,可育的转基因植物,由其产生的转基因种子,以及T1和随后的几代。