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11. 发明申请

US20170145497A1 METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS 审中-公开
公开(公告)号：US20170145497A1
公开(公告)日：2017-05-25
申请号：US15276709
申请日：2016-09-26
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan M. ROTHBERG , Wolfgang HINZ , Kim L. JOHNSON , James BUSTILLO , John LEAMON , Jonathan SCHULTZ
IPC分类号： C12Q1/68 , G01N27/414 , B01L3/00
CPC分类号： C12Q1/6874 , B01L3/502761 , B01L2300/046 , B01L2300/0663 , B01L2300/0851 , B01L2400/086 , C12Q1/6869 , G01N27/4145 , G01N27/4148 , C12Q2565/607 , C12Q2565/301 , C12Q2565/629
摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

12. 发明申请

US20160263578A1 Pinch Flow Regulator 有权
公开(公告)号：US20160263578A1
公开(公告)日：2016-09-15
申请号：US15162876
申请日：2016-05-24
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan SCHULTZ
IPC分类号： B01L3/00 , C12Q1/68
CPC分类号： F16K7/07 , B01L3/567 , B01L2200/06 , C12Q1/6869 , F16K7/04 , G05D16/0638 , G05D16/185
摘要： A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.

13. 发明申请

US20150361488A1 Sequencing Device 有权
标题翻译：排序设备
公开(公告)号：US20150361488A1
公开(公告)日：2015-12-17
申请号：US14742404
申请日：2015-06-17
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan SCHULTZ , Todd ROSWECH , Jon A. HOSHIZAKI , Albert L. CARRILLO , James A. BALL
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6869 , B01F1/0027 , B01F5/0683 , B01F11/0048 , B01F11/0071 , B01L3/502 , B01L3/505 , B01L3/527 , B01L3/563 , B01L2200/04 , B01L2200/16 , B01L2300/022 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , C12Q1/6806 , G01N27/4145 , G01N35/1002 , G01N35/1095 , Y10T436/11 , Y10T436/143333
摘要： A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
摘要翻译：制备试剂的方法包括将药筒插入仪器中。盒包括设置在盒的空腔中并将端口暴露于盒的外部的多个试剂盒。每个试剂盒包括试剂容器，其包括试剂和限定可压缩体积的内腔，通过试剂容器限定到内腔的开口。该方法还包括将多个流体端口连接到多个试剂盒的开口; 通过所述流体端口施加溶液以至少部分地填充所述多个试剂外壳; 并且循环空腔的压力，由此对于每个试剂外壳，在增加的压力期间，溶液进入试剂容器的内腔，与试剂结合，并压缩可压缩体积，并且在减压期间，可压缩体积减少，试剂通过开口排出。

14. 发明申请

US20150360227A1 Pinch Flow Regulator 有权
标题翻译：夹紧流量调节器
公开(公告)号：US20150360227A1
公开(公告)日：2015-12-17
申请号：US14740573
申请日：2015-06-16
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan SCHULTZ
IPC分类号： B01L3/00 , C12Q1/68
CPC分类号： F16K7/07 , B01L3/567 , B01L2200/06 , C12Q1/6869 , F16K7/04 , G05D16/0638 , G05D16/185
摘要： A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.
摘要翻译：用于调节流体流动的阀包括限定下腔的壳体底座，并且包括在下腔体内的夹紧结构，提供对下腔室的外部通路的气体入口，基础流体入口和基础流体出口。壳体盖限定上腔，并且包括盖流体入口和盖流体出口。覆盖流体入口与上腔和下腔之间的基础流体出口流体连通，并且盖流体出口提供从上腔体的外部通路。隔膜设置在壳体基座和壳体盖之间。压板设置在下腔体中，并且包括与夹紧结构相对设置的夹点。夹管在底部流体入口和下腔体中的基础流体出口之间流体连通。

15. 发明申请

US20140271402A1 FLUIDICS SYSTEM FOR SEQUENTIAL DELIVERY OF REAGENTS 有权
标题翻译：用于顺序递送试剂的流体系统
公开(公告)号：US20140271402A1
公开(公告)日：2014-09-18
申请号：US14291372
申请日：2014-05-30
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan SCHULTZ , David MARRAN
IPC分类号： B01L3/00
CPC分类号： B01L3/502715 , B01J2219/00389 , B01J2219/00394 , B01L3/502 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502769 , B01L3/502776 , B01L3/52 , B01L2200/16 , B01L2300/0636 , B01L2300/0645 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0877 , B01L2400/0487 , C12Q1/6869 , G01N27/447 , G01N35/1002 , G01N35/1097 , Y10T137/0318 , Y10T137/0424 , Y10T137/85938 , C12Q2565/629 , C12Q2527/119
摘要： The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion. The invention is particularly advantageous in apparatus for performing sensitive multistep reactions, such as pH-based DNA sequencing reactions.
摘要翻译：本发明提供了一种用于将不同流体引导到公共体积（例如反应室或流动池）的无源流体回路，而不会混合或交叉污染。通过流体回路的连接点，节点和通道的流动方向和流速由上游阀（例如打开或关闭）的状态，电路入口或上游储存器处的差分流体压力，流动阻力等来控制。通过所选择的入口流体的流动防止流体从未选择的入口到共同的出口或其他入口处的自由扩散或泄漏，其一部分由未选择的流体的入口扫过并且通过废气端口离开流体回路，从而产生阻止与出口流通过泄漏或扩散的不期望的混合的屏障。本发明在用于进行灵敏多步反应（例如基于pH的DNA测序反应）的装置中特别有利。

16. 发明申请

US20130280702A1 ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS 审中-公开
公开(公告)号：US20130280702A1
公开(公告)日：2013-10-24
申请号：US13859673
申请日：2013-04-09
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan SCHULTZ , John DAVIDSON
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

17. 发明申请

US20230133734A1 PHASE-PROTECTING REAGENT FLOW ORDERINGS FOR USE IN SEQUENCING-BY-SYNTHESIS 有权
公开(公告)号：US20230133734A1
公开(公告)日：2023-05-04
申请号：US17865019
申请日：2023-01-23
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Earl HUBBELL , Jonathan SCHULTZ
IPC分类号： C12Q1/6869 , C12Q1/6874
摘要： A system for nucleic acid sequencing includes a machine-readable memory and a processor configured to execute machine-readable instructions. The instructions, when executed by the processor, cause the system to expose template polynucleotide strands in a plurality of defined spaces of a sensor array to a series of flows of nucleotide species, the series comprising a sequence of random flows; and obtain, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.

18. 发明申请

US20200332358A1 WINDOWED SEQUENCING 审中-公开
公开(公告)号：US20200332358A1
公开(公告)日：2020-10-22
申请号：US16866653
申请日：2020-05-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Todd Rearick , Mark MILGREW , Jonathan SCHULTZ , Chris PAPALIAS , Kim JOHNSON
IPC分类号： C12Q1/6874 , G16B30/00
摘要： In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors.

19. 发明申请

US20190156918A1 MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS 审中-公开
公开(公告)号：US20190156918A1
公开(公告)日：2019-05-23
申请号：US16195068
申请日：2018-11-19
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Todd REARICK , Jonathan SCHULTZ
IPC分类号： G16B40/00 , C12Q1/6869
摘要： Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.

20. 发明申请

US20190033330A1 SYSTEM AND METHOD INCORPORATING SOLID BUFFER 审中-公开
公开(公告)号：US20190033330A1
公开(公告)日：2019-01-31
申请号：US16149799
申请日：2018-10-02
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： James A. BALL , Jonathan SCHULTZ
IPC分类号： G01N33/84 , C12Q1/6869 , B01J19/00
摘要： A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

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