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11. 发明授权

US5350684A Extremely halophilic methanogenic archaebacteria 失效
标题翻译：极度嗜盐的产甲烷古细菌
公开(公告)号：US5350684A
公开(公告)日：1994-09-27
申请号：US668157
申请日：1991-03-12
申请人： Naoki Nakatsugawa , Koki Horikoshi
发明人： Naoki Nakatsugawa , Koki Horikoshi
IPC分类号： C02F11/04 , C12N1/20 , C12N5/02 , C12P5/02 , C12R1/01
CPC分类号： C12R1/01 , C12P5/023 , Y02E50/343 , Y10S435/822
摘要： Methanohalococcus alcaliphilum having an optimum growth sodium chloride concentration ranging from about 2.5 to about 3 mole are herein disclosed. The bacteria are halophilic or halophilic and alkalophilic and can be used as the microorganisms which play a central role in the methods of methane fermentation to enhance the efficiency of such a method since the use of the microorganism makes it possible to carry out the method at an alkaline pH, in the presence of salts in a high concentration and at a low temperature.
摘要翻译：本文公开了具有约2.5至约3摩尔的最佳生长氯化钠浓度的甲烷卤代球菌。细菌是嗜盐的或嗜盐的和嗜碱性的，并且可以用作在甲烷发酵方法中发挥中心作用的微生物，以提高这种方法的效率，因为微生物的使用使得可以在碱性pH，在高浓度和低温的盐存在下。

12. 发明授权

US5342778A Microorganism belonging to the genus flavobacterium designated ferm BP-4010 失效
标题翻译：属于黄杆菌属的微生物，命名为BP-4010
公开(公告)号：US5342778A
公开(公告)日：1994-08-30
申请号：US950586
申请日：1992-09-25
申请人： Kazuhito Moriya , Koki Horikoshi
发明人： Kazuhito Moriya , Koki Horikoshi
IPC分类号： C02F1/40 , C02F3/00 , C02F3/34 , C12N1/20 , C12R1/20 , E02B15/10 , C12N1/26 , C12N1/00 , D06M16/00
CPC分类号： C12R1/20 , C02F3/34 , Y10S435/85
摘要： A microorganism belonging to the genus Flavobacterium possessing the capacity to decompose hydrocarbons, tolerance to sulfurous acids, tolerance to salinity, tolerance to organic solvents, and tolerance to pressure. The microorganism is a strain of the genus Flavobacterium DS-711 (FERM BP-4010).
摘要翻译：属于具有分解碳氢化合物的能力的微生物属的微生物，对亚硫酸的耐受性，对盐度的耐受性，对有机溶剂的耐受性和耐受性。微生物是属于黄杆菌属DS-711（FERM BP-4010）的菌株。

13. 发明授权

US5055406A Extremely halophilic methanogenic archaebacteria 失效
标题翻译：极其罕见的甲烷生物学家
公开(公告)号：US5055406A
公开(公告)日：1991-10-08
申请号：US255015
申请日：1988-10-07
申请人： Naoki Nakatsugawa , Koki Horikoshi
发明人： Naoki Nakatsugawa , Koki Horikoshi
IPC分类号： C02F11/04 , C12N1/20 , C12N5/02 , C12P5/02 , C12R1/01
CPC分类号： C12R1/01 , C12P5/023 , Y02E50/343 , Y10S435/822
摘要： Methanohalococcus alcaliphilum having an optimum growth sodium chloride concentration ranging from about 2.5 to about 3 mole are herein disclosed. The bacteria are halophilic or halophilic and alkalophilic and can be used as the microorganisms which play a central role in the methods of methane fermentation to enhance the efficiency of such a method since the use of the microorganism makes it possible to carry out the method at an alkaline pH, in the presence of salts in a high concentration and at a low temperature.

14. 发明授权

US4803074A FR-900848 substance and preparation thereof 失效
标题翻译： FR-900848物质及其制备方法
公开(公告)号：US4803074A
公开(公告)日：1989-02-07
申请号：US175297
申请日：1988-03-30
申请人： Masaru Yoshida , Koki Horikoshi
发明人： Masaru Yoshida , Koki Horikoshi
IPC分类号： A61K35/74 , A61P31/04 , C07G11/00 , C12P1/06 , C12R1/625 , C12P1/04
CPC分类号： C12P1/06
摘要： This invention relates to a compound, FR-900848, having antimicrobial activity against various microorganisms, to a process for the preparation of same and to a pharmaceutical composition comprising same.

15. 发明授权

US08153413B2 High alkaline protease and use thereof 失效
标题翻译：高碱性蛋白酶及其用途
公开(公告)号：US08153413B2
公开(公告)日：2012-04-10
申请号：US11719713
申请日：2005-11-16
申请人： Jie Lu , Zhi jun Li , Si Hung Vo , Yuji Hatada , Ken Takai , Susumu Ito , Koki Horikoshi
发明人： Jie Lu , Zhi jun Li , Si Hung Vo , Yuji Hatada , Ken Takai , Susumu Ito , Koki Horikoshi
IPC分类号： C12N9/00 , C12N9/92 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/52
摘要： The invention aims to provide a novel alkaline protease having peculiar properties such as high alkali activity, resistance to surfactants and calcium-dependent thermostability and exhibiting excellent performance in highly alkaline detergents, and a gene coding for the amino acid sequence thereof. There is provided an alkaline protease with such properties that an active pH range is from 5 to 13, an optimum pH is approximately 12.6, an optimum temperature is 70° C., no activity drop by heating is observed up to 65° C. at pH 10 and the optimum temperature and the thermostability are not affected by Ca2+ ions. Specifically, there is provided, for example, an alkaline protease having an amino acid sequence constituting a mature enzyme as represented by SEQ ID NO: 3 or an amino acid sequence resulting from deletion, substitution, situs inversus arrangement, addition or insertion of a part of amino acids thereof, or derived from Alkaliphillus transvaalensis. The protease cleaves 26 peptide bonds among 29 peptide bonds of acidic insulin B-chain.
摘要翻译：本发明的目的在于提供一种具有高碱性，耐表面活性和钙依赖性热稳定性等特性，在高碱性洗涤剂中表现出优异性能的新型碱性蛋白酶及其氨基酸序列的编码基因。提供了具有这样的性质的碱性蛋白酶，其活性pH范围为5至13，最佳pH为约12.6，最适温度为70℃，在65℃下不观察到加热下的活性降低 pH 10，最佳温度和热稳定性不受Ca2 +离子的影响。具体地，例如提供了具有SEQ ID NO：3所示的构成成熟酶的氨基酸序列的氨基酸序列的碱性蛋白酶或由缺失，取代，位置反转排列，添加或插入部分的氨基酸，或衍生自变形白菜（Alkaliphillus transvaalensis）。蛋白酶在酸性胰岛素B链的29个肽键中切割26个肽键。

16. 发明授权

US06695076B2 Method of coring crustal core sample, and antimicrobial polymeric gel and gel material used in the method 失效
标题翻译：取芯地核心样品的方法，以及该方法中使用的抗菌聚合物凝胶和凝胶材料
公开(公告)号：US06695076B2
公开(公告)日：2004-02-24
申请号：US10053407
申请日：2002-01-18
申请人： Noriaki Masui , Shigeru Deguchi , Kaoru Tsujii , Koki Horikoshi
发明人： Noriaki Masui , Shigeru Deguchi , Kaoru Tsujii , Koki Horikoshi
IPC分类号： E21B4902
CPC分类号： E21B25/08 , G01N2001/021
摘要： A method of taking a crustal core sample, wherein the crustal core sample is obtained in a state coated with an antimicrobial polymeric gel formed of a polymer obtained by polymerizing an antimicrobial monomer, preferably a quaternary ammonium salt compound. The polymer preferably contains a component derived from the antimicrobial monomer in a proportion of 1 to 10 mol%.
摘要翻译：一种摄取地壳芯样品的方法，其中所述地壳芯样品在涂覆有由通过聚合抗微生物单体，优选季铵盐化合物获得的聚合物形成的抗微生物聚合物凝胶的状态下获得。聚合物优选含有1〜10摩尔％的来自抗微生物单体的成分。

17. 发明授权

US5935827A Maltose phosphorylase, trehalose phosphorylase, novel strain of genus plesiomonas capable of producing these enzymes and process for producing trehalose 失效
标题翻译：麦芽糖磷酸化酶，海藻糖磷酸化酶，能够产生这些酶的新颖菌属Plesiomonas和生产海藻糖的方法
公开(公告)号：US5935827A
公开(公告)日：1999-08-10
申请号：US94583
申请日：1998-06-15
申请人： Masahiro Yoshida , Nobuyuki Nakamura , Koki Horikoshi
发明人： Masahiro Yoshida , Nobuyuki Nakamura , Koki Horikoshi
IPC分类号： C12N9/10 , C12P19/12 , C12N9/12 , C12N9/14 , C12N9/24
CPC分类号： C12N9/1051 , C12P19/12 , C12R1/01
摘要： Disclosed are a novel microorganism (FERM BP-5144) belonging to the genus Plesiomonas and having ability to produce maltose phosphorylase and trehalose phosphorylase required for the enzymatic production of trehalose and novel maltose phosphorylase and trehalose phosphorylase obtainable from the microorganism as well as a process for producing the enzymes. A novel process for enzymatically producing trehalose (O-.alpha.-D-glucopyranosyl-(1.fwdarw.1)-D-glucopyranoside) is also disclosed.
摘要翻译：本发明公开了一种属于扁球菌属的新型微生物（FERM BP-5144），具有产生可从微生物获得的海藻糖和新型麦芽糖磷酸化酶和海藻糖磷酸化酶所需的麦芽糖磷酸化酶和海藻糖磷酸化酶所需的能力，产生酶。还公开了一种用于酶促生产海藻糖（O-α-D-吡喃葡萄糖基 - （1-> 1）-D-吡喃葡萄糖苷）的新方法。

18. 发明授权

US5807719A Maltose phosohorylase, trehalose phosphorylase, Plesiomonas strain and preparation process of trehalose 失效
标题翻译：麦芽糖磷酸化酶，海藻糖磷酸化酶，细菌菌株和海藻糖的制备方法
公开(公告)号：US5807719A
公开(公告)日：1998-09-15
申请号：US893504
申请日：1997-07-11
申请人： Masahiro Yoshida , Nobuyuki Nakamura , Koki Horikoshi
发明人： Masahiro Yoshida , Nobuyuki Nakamura , Koki Horikoshi
IPC分类号： C12N9/10 , C12P19/12 , C12N9/12 , C12N9/14 , C12N9/24
CPC分类号： C12N9/1051 , C12P19/12 , C12R1/01
摘要： Disclosed are a novel microorganism (FERM BP-5144) belonging to the genus Plesiomonas and having ability to produce maltose phosphorylase and trehalose phosphorylase required for the enzymatic production of trehalose and novel maltose phosphorylase and trehalose phosphorylase obtainable from the microorganism as well as a process for producing the enzymes. A novel process for enzymatically producing trehalose (O-.alpha.-D-glucopyranosyl-(1.fwdarw.1)-D-glucopyranoside) is also disclosed.
摘要翻译：本发明公开了一种属于扁球菌属的新型微生物（FERM BP-5144），具有产生可从微生物获得的海藻糖和新型麦芽糖磷酸化酶和海藻糖磷酸化酶所需的麦芽糖磷酸化酶和海藻糖磷酸化酶所需的能力，产生酶。还公开了一种用于酶促生产海藻糖（O-α-D-吡喃葡萄糖基 - （1-> 1）-D-吡喃葡萄糖苷）的新方法。

19. 发明授权

US4444647A Process for the oil extraction from oil sand by using cyclodextrin 失效
标题翻译：通过使用环糊精从油砂中提取油的工艺
公开(公告)号：US4444647A
公开(公告)日：1984-04-24
申请号：US340501
申请日：1982-01-18
申请人： Koki Horikoshi , Ichiro Shibanai
发明人： Koki Horikoshi , Ichiro Shibanai
IPC分类号： C10G1/00 , C10G1/04
CPC分类号： C10G1/047
摘要： Process for the oil extraction from oil sand comprising: mixing oil sand, cyclodextrin and water with one another to prepare a suspension, leaving it to stand or centrifuging it to separate into an oil, a water and a sand layers, and then collecting the oil layer.
摘要翻译：从油砂提取油的方法包括：将油砂，环糊精和水彼此混合以制备悬浮液，使其静置或离心分离成油，水和砂层，然后收集油层。

20. 发明授权

US07790457B2 Solid cellulosic culture medium and process for producing the same 有权
标题翻译：固体纤维素培养基及其制备方法
公开(公告)号：US07790457B2
公开(公告)日：2010-09-07
申请号：US10591288
申请日：2005-02-28
申请人： Shigeru Deguchi , Mikiko Tsudome , Susumu Ito , Koki Horikoshi
发明人： Shigeru Deguchi , Mikiko Tsudome , Susumu Ito , Koki Horikoshi
IPC分类号： C12N5/00 , A61K9/00
CPC分类号： C12N1/22 , C08L1/02
摘要： The present invention relates to a solid medium containing as a medium-solidifying component a cellulose gel, in particular a cellulose gel which is a porous cellulose gel structure containing cellulose as the skeletal part and having a cellulose concentration of 0.01% or higher and a porosity of 50% or higher, as well as a process for producing the same. The solid medium of the invention can be obtained by dispersing cellulose in a solvent, especially an aqueous thiocyanate salt solution, stirring and/or heating the dispersion to dissolve the cellulose, subsequently cooling the solution and/or removing the solvent to cause the solution to gel, and permeating nutrients into the resultant cellulose gel.The solid medium usable under a wide range of culture conditions where conventional solid media such as agar medium cannot be used, as well as a method for producing the same is provided.
摘要翻译：本发明涉及一种固体培养基，其中含有纤维素凝胶，特别是作为纤维素作为骨架部分并且纤维素浓度为0.01％以上的多孔纤维素凝胶结构的纤维素凝胶的纤维素凝胶，为50％以上，以及其制造方法。本发明的固体培养基可以通过将纤维素分散在溶剂中，特别是硫氰酸盐水溶液中，搅拌和/或加热分散体以溶解纤维素，随后冷却溶液和/或除去溶剂，凝胶，并将渗透的营养物质加入所得的纤维素凝胶中。提供了在不能使用常规固体培养基如琼脂培养基的广泛培养条件下可使用的固体培养基及其制备方法。

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