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11. 发明授权

US07981637B2 Fluorescent protein and chromoprotein 失效
标题翻译：荧光蛋白和荧光蛋白
公开(公告)号：US07981637B2
公开(公告)日：2011-07-19
申请号：US12976579
申请日：2010-12-22
申请人： Atsushi Miyawaki , Satoshi Karasawa
发明人： Atsushi Miyawaki , Satoshi Karasawa
IPC分类号： C12P21/06 , C12N15/00 , C12N1/20 , C07H21/04
CPC分类号： G01N33/542 , C07K14/37 , C07K2319/00 , G01N33/533 , G01N33/582 , G01N2021/6439 , G01N2500/10
摘要： It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from Anthopleura inornata, which have certain property, and fluorescent proteins from Trachyphyllia geoffroyi and Scolymia vitiensis, which have certain fluorescent property.
摘要翻译：本发明的目的是提供一种新型的生色蛋白和新的荧光蛋白。本发明提供了具有一定性质的Anthopleura inornata衍生的色蛋白，以及具有一定荧光性质的来自Trachyphyllia geoffroyi和Scolymia vitiensis的荧光蛋白。

12. 发明授权

US07956172B2 Fluorescent protein and chromoprotein 失效
标题翻译：荧光蛋白和荧光蛋白
公开(公告)号：US07956172B2
公开(公告)日：2011-06-07
申请号：US10561041
申请日：2004-06-16
申请人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
发明人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
IPC分类号： C12N15/12
CPC分类号： C07K14/43504
摘要： It is an object of the present invention to provide a novel fluorescent protein and a novel chromoprotein. The present invention provides a novel fluorescent protein derived from Montipora sp., Acropora sp. and Lobophytum crassum, and a novel chromoprotein derived from Actinia equine.
摘要翻译：本发明的目的是提供一种新的荧光蛋白和新的色蛋白。本发明提供了一种新型的从Montipora sp。，Acropora sp。和Lobophytum crassum，以及衍生自猕猴桃的新型染色体蛋白。

13. 发明授权

US07449151B2 Fluorescence resonance energy transfer analyzer 失效
标题翻译：荧光共振能量转移分析仪
公开(公告)号：US07449151B2
公开(公告)日：2008-11-11
申请号：US10753569
申请日：2004-01-09
申请人： Masahiko Hirano , Masafumi Oshiro , Atsushi Miyawaki
发明人： Masahiko Hirano , Masafumi Oshiro , Atsushi Miyawaki
IPC分类号： G01N33/00
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6417 , G01N2021/6421
摘要： An analyzer for measuring an FRET (fluorescence resonance energy transfer) efficiency of a specimen containing a donor and an acceptor. The analyzer has an illuminator, an optical system, a detector and a calculator. The illuminator emits light for donor excitation and acceptor bleaching. The detector detects fluorescence from the specimen. The calculator calculates the FRET efficiency using the output of the detector. The detector independently detects the fluorescence in wavelength regions. One of the regions has a larger overlap with the fluorescence spectrum of the acceptor than with that of the donor.
摘要翻译：用于测量含有供体和受体的样品的FRET（荧光共振能量转移）效率的分析仪。分析仪具有照明器，光学系统，检测器和计算器。照明器发光，用于供体激发和受主漂白。检测器检测样品的荧光。计算器使用检测器的输出计算FRET效率。检测器独立地检测波长区域中的荧光。其中一个区域与受体的荧光光谱重叠比与供体的荧光光谱重叠。

14. 发明申请

US20070207452A1 Method of detecting micronucleus in cell 审中-公开
标题翻译：检测细胞中微核的方法
公开(公告)号：US20070207452A1
公开(公告)日：2007-09-06
申请号：US11729529
申请日：2007-03-29
申请人： Hirobumi Suzuki , Atsushi Miyawaki
发明人： Hirobumi Suzuki , Atsushi Miyawaki
IPC分类号： C12Q1/00 , C12Q1/68
CPC分类号： G01N33/5017
摘要： The present invention provides a method of detecting a micronucleus in a living cell to be measured, without having to fixate the cell, characterized by comprising generating a mother cell by introducing a gene encoding a single or a plurality of nucleus-related proteins and a gene encoding a fluorescence protein into a culture cell, and making then expressed in the cell, subjecting the cell to a treatment for a toxicity test or a mutagenicity test of a test substance, performing a limited excitation which makes a region of the micronucleus emit fluorescence limitedly, and quantitatively measuring an amount of the fluorescence corresponding to a component of the nucleus-related protein.
摘要翻译：本发明提供一种检测待测细胞中的微核的方法，而不必固定细胞，其特征在于包括通过引入编码单个或多个核相关蛋白的基因和基因产生母细胞将荧光蛋白编码到培养细胞中，然后在细胞中表达，对细胞进行毒性试验或试验物质的诱变试验处理，进行有限激发，使微核区域有限地发射荧光并定量测定与核相关蛋白质的成分相对应的荧光量。

15. 发明申请

US20060240472A1 Pigment protein 失效
标题翻译：颜料蛋白
公开(公告)号：US20060240472A1
公开(公告)日：2006-10-26
申请号：US10516317
申请日：2003-06-10
申请人： Atsushi Miyawaki , Satoshi Karasawa
发明人： Atsushi Miyawaki , Satoshi Karasawa
IPC分类号： G01N33/53 , C07H21/04 , C12P21/06 , C07K14/435
CPC分类号： G01N33/542 , C08L23/02
摘要： An object of the present invention is to provide a novel chromoprotein derived from Cnidopus japonicus. The present invention provides a chromoprotein derived from Cnidopus japonicus having the following properties: (1) the absorption maximum wavelength is 610 nm, and fluorescence is not emitted; (2) the molar absorption coefficient is 66,700 at 610 nm; and (3) the pH sensitivity of light-absorbing property is stable at between pH 4 and pH 10.
摘要翻译：本发明的一个目的是提供一种衍生自日本n a属的新型生色蛋白。本发明提供了具有以下特性的源自日本蝉的色素蛋白：（1）吸收最大波长为610nm，荧光不发射; （2）610 nm处的摩尔吸光系数为66,700; 和（3）在pH4和pH10之间，光吸收性质的pH敏感性是稳定的。

16. 发明申请

US20050106661A1 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US20050106661A1
公开(公告)日：2005-05-19
申请号：US10492081
申请日：2002-10-10
申请人： Atsushi Miyawaki , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Satoshi Karasawa , Toshio Araki
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C07H21/04
CPC分类号： C07K14/43504
摘要： An object of the present invention is to provide a novel fluorescent protein derived from organisms other than Aequorea victoria. According to the present invention, there is provided a fluorescent protein derived from Galaxea fascicularis, which has the following properties: (1) the molecular weight is approximately 27,000; (2) a tetramer is formed in an equilibration state; (3) the excitation maximum wavelength is 492 nm, and the fluorescence maximum wavelength is 505 nm; (4) the molar absorption coefficient is 74,100; (5) the quantum yield is 0.625; and (6) the pH sensitivity of the fluorescent property is low in the range between pH 5 and pH 12.
摘要翻译：本发明的目的是提供一种源自维他命A（Aequorea victoria）以外的生物体的新型荧光蛋白。根据本发明，提供了一种衍生自Galaxea fascicularis的荧光蛋白，其具有以下性质：（1）分子量约为27,000; （2）在平衡状态下形成四聚体; （3）激发最大波长为492nm，荧光最大波长为505nm; （4）摩尔吸光系数为74,100; （5）量子产率为0.625; 和（6）pH5和pH12之间的荧光性能的pH敏感性低。

17. 发明授权

US06673610B2 Method for mutagenesis 失效
标题翻译：诱变方法
公开(公告)号：US06673610B2
公开(公告)日：2004-01-06
申请号：US09920922
申请日：2001-08-02
申请人： Atsushi Miyawaki , Asako Sawano
发明人： Atsushi Miyawaki , Asako Sawano
IPC分类号： C12N1500
CPC分类号： C12N15/102 , C12Q1/6827 , C12Q2537/155 , C12Q2525/185
摘要： The present invention provides an entirely new method for mutagenesis, which is simple, speedy, economical, and widely-applicable. A method for mutagenesis comprising steps of: DNA synthesis in which primers which have mutations and a phosphorylated 5′-terminus are annealed to a template DNA and then subjected to an elongation reaction using a thermostable high-fidelity DNA polymerase, after which the phosphorylated 5′-terminus and the elongated terminus are ligated by means of a thermostable DNA ligase to synthesize a circular DNA containing said primers; digestion in which at least DNAs other than the amplified circular DNA are digested into several fragments; and double-stranded DNA synthesis in which, with the several fragments obtained in the above step of digestion as megaprimers, said megaprimers are annealed to said circular DNA synthesized in the above step of DNA synthesis, followed by an elongation reaction performed using said thermostable high-fidelity DNA polymerase.
摘要翻译：本发明提供了一种全新的诱变方法，其简单，快速，经济且广泛适用。一种诱变方法，包括以下步骤：DNA合成，其中具有突变和磷酸化5'-末端的引物退火模板DNA，然后使用热稳定性高保真DNA聚合酶进行延伸反应，然后通过热稳定DNA连接酶连接磷酸化的5'末端和细长末端，以合成含有所述引物的环状DNA;消化其中至少将经扩增的环状DNA以外的DNA消化成几个片段; 和双链DNA合成，其中在上述消化步骤中获得的几个片段作为大引物，所述大引物与上述DNA合成步骤中合成的所述环状DNA退火，然后使用所述耐热高分子量聚合物进行伸长反应，保真DNA聚合酶。

18. 发明授权

US09249449B2 Probe reagent for measurement of proteolytic activity 有权
标题翻译：用于测量蛋白水解活性的探针试剂
公开(公告)号：US09249449B2
公开(公告)日：2016-02-02
申请号：US13574400
申请日：2011-01-21
申请人： Atsushi Miyawaki , Masahiko Hirano
发明人： Atsushi Miyawaki , Masahiko Hirano
IPC分类号： C12Q1/37 , C12N15/85
CPC分类号： C12Q1/37 , C07K2319/00 , C12N15/85
摘要： This invention relates to a probe reagent comprising, in order from the N-terminus to the C-terminus, the amino acid sequences of a fluorescent protein I, a peptide capable of terminating protein degradation (i.e., a degradation-terminating peptide), a spacer peptide, a fluorescent protein II, and a protein to be degraded, wherein the protein to be degraded is a protein degraded by the ubiquitin-proteasome system, and the probe reagent is degraded from the C-terminus, but that the degradation of the probe reagent is terminated at the degradation-terminating peptide, a nucleic acid encoding the probe reagent, and use of the probe reagent or the nucleic acid.
摘要翻译：本发明涉及探针试剂，其从N末端到C末端的顺序包括荧光蛋白I的氨基酸序列，能够终止蛋白质降解的肽（即降解终止肽），间隔肽，荧光蛋白II和待降解的蛋白质，其中待降解的蛋白质是由泛素 - 蛋白酶体系统降解的蛋白质，并且探针试剂从C末端降解，但是探针试剂终止于降解终止肽，编码探针试剂的核酸和探针试剂或核酸的使用。

19. 发明授权

US08378077B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US08378077B2
公开(公告)日：2013-02-19
申请号：US13098999
申请日：2011-05-02
申请人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
发明人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
IPC分类号： C07K14/435 , C07H21/04
CPC分类号： C07K14/43595
摘要： An object of the present invention is to provide a novel fluorescent protein derived from favia favus. The present invention provides a fluorescent protein derived from favia favus having the following properties: (1) an excitation maximum wavelength is 507 nm; (2) a fluorescence maximum wavelength is 517 nm; (3) a molar absorption coefficient at 482 nm is 80,000; (4) a quantum yield is 0.68; and (5) pH sensitivity of the fluorescence maximum is stable at pH 5 to pH 11.
摘要翻译：本发明的一个目的是提供一种来源于粉刺的新型荧光蛋白。本发明提供具有以下特性的源自粉刺的荧光蛋白：（1）激发最大波长为507nm; （2）荧光最大波长为517nm; （3）在482nm处的摩尔吸光系数为80,000; （4）量子产率为0.68; 和（5）荧光最大的pH敏感性在pH5至pH11下是稳定的。

20. 发明授权

US08182987B2 Probe for visualizing cell-cycle 有权
标题翻译：探测细胞周期可视化
公开(公告)号：US08182987B2
公开(公告)日：2012-05-22
申请号：US12450155
申请日：2008-02-06
申请人： Atsushi Miyawaki , Asako Sawano , Hisao Masai
发明人： Atsushi Miyawaki , Asako Sawano , Hisao Masai
IPC分类号： C12N15/63 , C12Q1/68 , G01N33/53 , G01N33/542
CPC分类号： G01N33/50 , A01K67/0275 , A01K2207/12 , A01K2217/05 , A01K2227/105 , A01K2267/0331 , A01K2267/0393 , A61K49/0008 , C07K14/4702 , C12N15/8509 , G01N33/5005
摘要： An object of an embodiment of the present invention is to provide a method with which it is possible to easily distinguish a proliferation phase of a cell cycle from a resting phase thereof in real time. The object of the embodiment of the present invention is attained by providing a method for performing phase identification of the cell cycle, the method including: visualizing one or more gene-expression products as markers whose amounts in a cell change in a cell-cycle dependent manner; and detecting the products so as to distinguish the proliferation phase of the cell cycle from the resting phase thereof.
摘要翻译：本发明的一个实施方案的目的是提供一种可以实时地容易地将细胞周期的增殖期与其静止期区分开的方法。本发明的实施方案的目的是通过提供一种用于进行细胞周期的相位鉴定的方法来实现，所述方法包括：将一种或多种基因表达产物可视化为其细胞中细胞周期依赖性变化的标记方式; 并检测产物以区分细胞周期的增殖期与其静止期。

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