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11. 发明申请

US20060254503A1 Seed layers, cap layers, and thin films and methods of making thereof 有权
标题翻译：种子层，盖层和薄膜及其制造方法
公开(公告)号：US20060254503A1
公开(公告)日：2006-11-16
申请号：US11433824
申请日：2006-05-15
申请人： Haixia Dai , Khashayar Pakbaz , Michael Spaid , Theo Nikiforov
发明人： Haixia Dai , Khashayar Pakbaz , Michael Spaid , Theo Nikiforov
IPC分类号： C30B23/00
CPC分类号： C23C18/1608 , B82Y30/00 , C23C18/1844 , C23C18/50 , H01L21/288 , H01L21/76814 , H01L21/76843 , H01L21/76849 , H01L21/7685 , H01L21/76861 , H01L21/76864 , H01L21/76867 , H01L21/76868 , H01L21/76871 , H01L21/76874 , H01L21/76879
摘要： A seed layer is formed on a substrate using a first biological agent. The seed layer may comprise densified nanoparticles which are bound to the biological agent. The seed layer is then used for a deposition of a metal layer, such as a barrier layer, an interconnect layer, a cap layer and/or a bus line for a solid state device.
摘要翻译：使用第一生物试剂在基材上形成种子层。种子层可以包含与生物制剂结合的致密化纳米颗粒。然后种子层用于沉积用于固态器件的金属层，例如阻挡层，互连层，盖层和/或总线。

12. 发明申请

US20060068410A1 Methods and systems for monitoring intracellular binding reactions 审中-公开
公开(公告)号：US20060068410A1
公开(公告)日：2006-03-30
申请号：US11070155
申请日：2005-03-01
申请人： H. Wada , Javier Farinas , Theo Nikiforov
发明人： H. Wada , Javier Farinas , Theo Nikiforov
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6841 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/5008 , G01N33/5041 , G01N33/542 , G01N33/582 , G01N33/6872 , C12Q2561/119
摘要： Intracellular binding reactions, and particularly DNA/DNA binding protein reactions are detected in situ, using intracellular fluorescence polarization detection. The methods comprise providing a biological cell having at least a first component of a binding reaction disposed therein. The cell is contacted with a second component of the binding reaction whereby the second component is internalized within the biological cell. At least one of the first and second components has a fluorescent label. The amount of binding between the first and second components within the cell is determined by measuring a level of polarized and/or depolarized fluorescence emitted from within the biological cell.

13. 发明申请

US20060219557A1 Methods and systems for reducing background signal in assays 审中-公开
公开(公告)号：US20060219557A1
公开(公告)日：2006-10-05
申请号：US11411992
申请日：2006-04-25
申请人： Theo Nikiforov , Aileen Zhou
发明人： Theo Nikiforov , Aileen Zhou
IPC分类号： C07K1/26
CPC分类号： G01N27/44726
摘要： Methods and systems of monitoring reactions, e.g., assays, that filter out background signal from substrate, in detecting the product. The methods and systems controllably move detectable reaction product from a first location to a second location at which the product is detected while controllably moving potentially interfering substrate materials away from or not toward the second location. Controllable movement of the different species is accomplished through the controlled combination of bulk fluid flow and differential electrophoresis of substrate and product to move the product, but not the substrate past the detection region.

14. 发明申请

US20050176071A1 Homogeneous assay methods 审中-公开
标题翻译：均相测定方法
公开(公告)号：US20050176071A1
公开(公告)日：2005-08-11
申请号：US10855657
申请日：2004-05-27
申请人： Theo Nikiforov , Sang Jeong
发明人： Theo Nikiforov , Sang Jeong
IPC分类号： C12Q1/48 , G01N33/542 , G01N33/543 , G01N33/53
CPC分类号： C12Q1/485 , G01N33/542 , G01N33/54306
摘要： The present invention provides a method of assaying for kinase activity, comprising contacting a fluorescently labeled phosphorylatable peptide substrate with an ATP analog in the presence of a kinase enzyme to yield a first product; contacting the first product with a reactant that comprises a biotin derivative to yield a second product; contacting the second product with a biotin-binding protein; and detecting a difference in a fluorescence polarization level from the second product as compared to a fluorescence polarization of the peptide substrate.
摘要翻译：本发明提供了测定激酶活性的方法，包括在激酶的存在下使荧光标记的可磷酸化肽底物与ATP类似物接触以产生第一产物; 使第一产物与包含生物素衍生物的反应物接触以产生第二产物; 使第二产物与生物素结合蛋白接触; 并且与肽基板的荧光偏振相比，检测来自第二产物的荧光偏振电平的差异。

15. 发明申请

US20060254504A1 Plating bath and surface treatment compositions for thin film deposition 失效
标题翻译：电镀液和表面处理组合物用于薄膜沉积
公开(公告)号：US20060254504A1
公开(公告)日：2006-11-16
申请号：US11433825
申请日：2006-05-15
申请人： Haixia Dai , Khashayar Pakbaz , Michael Spaid , Theo Nikiforov
发明人： Haixia Dai , Khashayar Pakbaz , Michael Spaid , Theo Nikiforov
IPC分类号： C30B23/00
CPC分类号： C23C18/1608 , B82Y30/00 , C23C18/1844 , C23C18/50 , H01L21/288 , H01L21/76814 , H01L21/76843 , H01L21/76849 , H01L21/7685 , H01L21/76861 , H01L21/76864 , H01L21/76867 , H01L21/76868 , H01L21/76871 , H01L21/76874 , H01L21/76879
摘要： An aqueous substrate surface treatment composition includes cysteine and an acidic solution having a pH of about 7 or less. The composition enables a selective deposition of a metal ion sensitizer and a subsequent selective plating of a metallic cap layer. Various CoWP plating bath compositions are also provided which may be used to form the cap layer.
摘要翻译：水性底物表面处理组合物包含半胱氨酸和pH为约7或更小的酸性溶液。该组合物能够选择性沉积金属离子敏化剂并随后选择性镀覆金属覆盖层。还提供了可用于形成盖层的各种CoWP电镀浴组合物。

16. 发明申请

US20050009174A1 Detection of nucleic acid hybridization by fluorescence polarization 审中-公开
标题翻译：通过荧光偏振检测核酸杂交
公开(公告)号：US20050009174A1
公开(公告)日：2005-01-13
申请号：US10881000
申请日：2004-06-30
申请人： Theo Nikiforov , Sang Jeong
发明人： Theo Nikiforov , Sang Jeong
IPC分类号： B01L3/00 , C07H21/02 , C07H21/04 , C12M1/34 , C12P19/34 , C12Q1/68 , C12Q1/6816 , C12Q1/6825 , C12Q1/6827 , G01N21/64
CPC分类号： C12Q1/6816 , B01L3/5027 , B01L2400/0415 , C12Q1/6825 , C12Q1/6827 , G01N21/6428 , G01N21/6445 , G01N21/6452 , G01N21/6458 , G01N2021/6482 , C12Q2565/629 , C12Q2561/119 , C12Q2527/125 , C12Q2525/107
摘要： Methods, systems and assays are provided for FP detection of nucleic acid hybridization.
摘要翻译：提供了用于核酸杂交的FP检测的方法，系统和测定。

17. 发明授权

US07655081B2 Plating bath and surface treatment compositions for thin film deposition 失效
标题翻译：电镀液和表面处理组合物用于薄膜沉积
公开(公告)号：US07655081B2
公开(公告)日：2010-02-02
申请号：US11433825
申请日：2006-05-15
申请人： Haixia Dai , Khashayar Pakbaz , Michael Spaid , Theo Nikiforov
发明人： Haixia Dai , Khashayar Pakbaz , Michael Spaid , Theo Nikiforov
IPC分类号： C23C18/34 , C23C18/36
CPC分类号： C23C18/1608 , B82Y30/00 , C23C18/1844 , C23C18/50 , H01L21/288 , H01L21/76814 , H01L21/76843 , H01L21/76849 , H01L21/7685 , H01L21/76861 , H01L21/76864 , H01L21/76867 , H01L21/76868 , H01L21/76871 , H01L21/76874 , H01L21/76879
摘要： An aqueous substrate surface treatment composition includes cysteine and an acidic solution having a pH of about 7 or less. The composition enables a selective deposition of a metal ion sensitizer and a subsequent selective plating of a metallic cap layer. Various CoWP plating bath compositions are also provided which may be used to form the cap layer.
摘要翻译：水性底物表面处理组合物包含半胱氨酸和pH为约7或更小的酸性溶液。该组合物能够选择性沉积金属离子敏化剂并随后选择性镀覆金属覆盖层。还提供了可用于形成盖层的各种CoWP电镀浴组合物。

18. 发明授权

US5952174A Ligase/polymerase-mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis 失效
标题翻译：单核苷酸多态性的连接酶/聚合酶介导的遗传位分析及其在遗传分析中的应用
公开(公告)号：US5952174A
公开(公告)日：1999-09-14
申请号：US929101
申请日：1997-09-15
申请人： Theo Nikiforov , Jonathan Karn , Philip Goelet
发明人： Theo Nikiforov , Jonathan Karn , Philip Goelet
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q1/6862 , Y10S435/81
摘要： A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.
摘要翻译：提供了用于确定核酸分子中预选位点处的核苷酸的身份的方法。该方法包括将与预选位点处存在的核苷酸互补的核苷三磷酸引入引物分子的末端，并且随后与第二寡核苷酸连接。通过检测连接到反应的固相的特定标记或通过在溶液中检测来监测反应。

19. 发明授权

US5679524A Ligase/polymerase mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis 失效
标题翻译：单核苷酸多态性的连接酶/聚合酶介导的遗传位分析及其在遗传分析中的应用
公开(公告)号：US5679524A
公开(公告)日：1997-10-21
申请号：US694835
申请日：1996-08-09
申请人： Theo Nikiforov , Jonathan Karn , Philip Goelet
发明人： Theo Nikiforov , Jonathan Karn , Philip Goelet
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q1/6862 , Y10S435/81
摘要： A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.
摘要翻译：提供了用于确定核酸分子中预选位点处的核苷酸的身份的方法。该方法包括将与预选位点处存在的核苷酸互补的核苷三磷酸引入引物分子的末端，并且随后与第二寡核苷酸连接。通过检测连接到反应的固相的特定标记或通过在溶液中检测来监测反应。

20. 发明授权

US5610287A Method for immobilizing nucleic acid molecules 失效
标题翻译：固定核酸分子的方法
公开(公告)号：US5610287A
公开(公告)日：1997-03-11
申请号：US341148
申请日：1994-11-16
申请人： Theo Nikiforov , Michael R. Knapp
发明人： Theo Nikiforov , Michael R. Knapp
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/04 , C12N11/08 , C12N15/00
CPC分类号： C12Q1/6834 , C12N15/1006
摘要： Synthetic nucleic acid molecules are non-covalently immobilized in the presence of a salt or cationic detergent on a hydrophilic polystyrene solid support containing an --OH, --C.dbd.O or --COOH hydrophilic group or on a glass solid support. The support is contacted with a solution having a pH of about 6 to about 8 containing the synthetic nucleic acid and the cationic detergent or salt. Preferably, the cationic detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride at a concentration of about 30 mM to about 100 mM or octyldimethylamine hydrochloride at a concentration of about 50 mM to about 150 mM. The salt is preferably NaCl at a concentration of about 50 mM to about 250 mM. When the detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride, the glass support or the hydrophilic polystyrene support is used. When NaCl or octyldimethylamine hydrochloride is used, the support is the hydrophilic polystyrene. After immobilization, the support containing the immobilized nucleic acid may be washed with an aqueous solution containing a non-ionic detergent. The immobilized nucleic acid may be used in nucleic acid hybridization assays, nucleic acid sequencing and in analysis of genomic polymorphisms.
摘要翻译：在含有-OH，-C = O或-COOH亲水基团的亲水性聚苯乙烯固体载体上或在玻璃固体载体上，在盐或阳离子洗涤剂存在下，合成核酸分子非共价固定。将载体与含有合成核酸和阳离子洗涤剂或盐的pH约6至约8的溶液接触。优选地，阳离子洗涤剂是浓度为约30mM至约100mM的1-乙基-3-（3'-二甲基氨基丙基）-1,3-碳二亚胺氯化物或浓度为约50mM至约150mM的辛基二甲基胺盐酸盐。该盐优选浓度为约50mM至约250mM的NaCl。当洗涤剂为1-乙基-3-（3'-二甲基氨基丙基）-1,3-碳二亚胺氯化物时，使用玻璃载体或亲水性聚苯乙烯载体。当使用NaCl或辛基二甲胺盐酸盐时，载体是亲水性聚苯乙烯。固定后，含有固定化核酸的载体可以用含有非离子型洗涤剂的水溶液洗涤。固定核酸可用于核酸杂交测定，核酸测序和基因组多态性分析。

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