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11. 发明专利

JP2015050983A 細胞培養装置及び細胞培養方法有权
标题翻译：细胞培养装置和细胞培养方法
公开(公告)号：JP2015050983A
公开(公告)日：2015-03-19
申请号：JP2013186430
申请日：2013-09-09
申请人：株式会社日立製作所 , Hitachi Ltd
发明人： NANBA MASARU , HAGA RYOICHI , MURAKAMI SEI , SUZUKI HARUO
IPC分类号： C12M1/00
CPC分类号： C12M41/30 , C12M23/14 , C12M23/22 , C12M23/26 , C12M37/00 , C12M41/36
摘要：【課題】外部から無菌バッグ内に濁度センサを挿入することなしに細胞培養液の濁度を連続的且つ正確に測定し、細胞を培養することのできる細胞培養装置を提供する。【解決手段】細胞培養装置1は、装置内の所定の位置にセットされ、光透過性と可撓性を有し、内部で細胞培養液4を培養する無菌バッグ2と、前記無菌バッグ2の一部2aを介して前記無菌バッグ2内の細胞培養液4に光を照射する光照射部31、及び、前記無菌バッグ2の他の一部2bを介して前記無菌バッグ2内の細胞培養液4を透過した光を受光する光受光部32を有し、前記光照射部31、前記無菌バッグ2の一部2a、前記無菌バッグ2の他の一部2b及び前記光受光部32を光学的に一直線に配置した濁度センサ部3と、を備えることを特徴とする。【選択図】図1
摘要翻译：要解决的问题：提供一种连续精确地测量细胞培养液浊度的细胞培养装置，而不将浊度传感器从外部插入无菌袋中，并可培养细胞。解决方案：细胞培养装置1包括：设置在设备中规定位置的无菌袋2具有透光性和柔韧性，并在其中培养细胞培养液4; 以及具有通过无菌袋2的部分2a将无菌袋2中的细胞培养液4照射到无菌袋2中的光照射部31的浊度传感器部分3和接收通过细胞培养液的光的受光部32 4的无菌袋2通过无菌袋2的另一部分2b，并将光照射部31，无菌袋2的部分2a，无菌袋2的另一部分2b和光接收部32配置在光学直线。

12. 发明专利

JPH08299923A WASHING METHOD AND WASHER 失效
公开(公告)号：JPH08299923A
公开(公告)日：1996-11-19
申请号：JP11282395
申请日：1995-05-11
申请人： HITACHI LTD
发明人： HAGA RYOICHI , NITSUTA MICHIYO , MURAKAMI SEI
IPC分类号： B08B3/10
摘要： PURPOSE: To prevent defective washing when the composition, the temperature, the flow rate and the washing time of a washing liquid is set in advance to wash a material to be washed by preparing change pattern with time in which various conditions in a case when washing had suitably been performed in advance are fetched and performing washing while comparing the actually measured value with the change pattern. CONSTITUTION: Washing work of a pipe line 53 partitioned by valves 42, 45, 46, 47, 48 is carried out in compliance with work procedures prepared based on the results of a washing test made in advance. That is, first, a washing liquid is prepared in a washing liquid preparing tank 1 based on the work procedures and is heated to a prescribed temp. Next, the washing liquid is forcibly fed by a pump 5 with the opening degree of a valve 19 being controlled so that the flow rate in the pipe line 53 becomes a prescribed value. During washing,by a flow meter 9, a temperature gage 10, a pH meter 11, conductometric devices 12, 13, flow meters 21, 22 and temperature gages 24, 26, information on washing is collected with time to monitor the washing conditions. From a point of time when all the monitors satisfy the prescribed washing conditions, washing is continued for a set time.

13. 发明专利

JPH08252550A WASHING METHOD INSIDE OF PIPING 失效
公开(公告)号：JPH08252550A
公开(公告)日：1996-10-01
申请号：JP5853595
申请日：1995-03-17
申请人： HITACHI LTD
发明人： NAKANO TAKAMORI , MURAKAMI SEI , MATSUOKA TATSUHIKO
IPC分类号： B08B9/02 , B08B9/027 , B08B9/06
摘要： PURPOSE: To wash the inside of a piping with an impact stream generated in the piping by causing a flow rate change of a supplied washing water in a pharmaceutical, food and chemical plant, especially in a washing method of the inside of the piping and an equipment. CONSTITUTION: A device used is composed of the piping connecting a pump 1 provided with an inverter, supplying a washing liq. and capable of changing flow rate or a regulating valve 2 capable of changing the flow rate by an opening degree of the valve and a washing water tank 6 storing washing water 7. Therefore, an effect due to the impact stream by vacuum is added to a scientific effect by a conventional washing effect and an effect due to dilution, and the amount of washing water to be used and the washing time are reduced.

14. 发明专利

JPH08192125A TANK CLEANING SYSTEM 失效
公开(公告)号：JPH08192125A
公开(公告)日：1996-07-30
申请号：JP307295
申请日：1995-01-12
申请人： HITACHI LTD
发明人： MATSUOKA TATSUHIKO , MURAKAMI SEI , NAKANO TAKAMORI
IPC分类号： B08B9/093
摘要： PURPOSE: To provide a tank cleaning system which detects that cleaning liquid is ejected normally from a spray ball to hit the inner surface of a tank and verifies that cleaning in the tank is carried out correctly in cleaning CIP in plants for chemical products, foods, and pharmaceuticals, etc. CONSTITUTION: It is detected by a sensor and recorded by a recorder that cleaning liquid is ejected correctly from a spray ball 2 to the inner surface of a tank to be cleaned to validate that cleaning is conducted normally. In this way, it is recorded that at least the cleaning liquid is ejected normally in cleaning CIP in a plant, and data can be obtained to verify that cleaning is conducted normally.

15. 发明专利

JPH0880183A CULTURE DEVICE 失效
公开(公告)号：JPH0880183A
公开(公告)日：1996-03-26
申请号：JP21693494
申请日：1994-09-12
申请人： HITACHI LTD
发明人： NAKANO TAKAMORI , MURAKAMI SEI , MATSUOKA TATSUHIKO
IPC分类号： C12M1/00
摘要： PURPOSE: To provide a culture device having a simple structure and substantially not contaminated with various microbes. CONSTITUTION: Drain pipings from an aseptic system 7 are assembled and connected to a semi-aseptic header 5 to prevent the direct contact of the aseptic system 7 to a nonaseptic system 8. The semi-aseptic header 5 is connected to a steam piping so that the semi-aseptic header can be flushed with steam. Thereby, the contamination of the aseptic system with various microbes from drains can be prevented.

16. 发明专利

JPH0856645A CULTURE DEVICE FOR PREVENTING CONTAMINATION FROM WASTE LIQUOR 失效
公开(公告)号：JPH0856645A
公开(公告)日：1996-03-05
申请号：JP19515494
申请日：1994-08-19
申请人： HITACHI LTD
发明人： NAKANO TAKAMORI , MURAKAMI SEI
IPC分类号： C12M1/00
摘要： PURPOSE: To provide a culture device of simple constitution being hardly contaminated with miscellaneous microorganisms in a culture device requiring asepsis. CONSTITUTION: Drain pipes from an aseptic system 7 are collectively connected to a subaseptic header 5. Anon-aseptic system 8 is made not to directly come into contact with the aseptic system 7. The subaseptic header 5 is connected to a steam piping so that steam flash is made possible. Contamination with miscellaneous microorganisms can be prevented.

17. 发明专利

JPH07190883A METHOD AND APPARATUS FOR PREPARATION OF MEDICINE, ETC. 失效
公开(公告)号：JPH07190883A
公开(公告)日：1995-07-28
申请号：JP33060293
申请日：1993-12-27
申请人： HITACHI LTD
发明人： MATSUOKA TATSUHIKO , MURAKAMI SEI , NAKANO TAKAMORI
IPC分类号： G01M3/04 , A61J3/00 , G01M3/28
摘要： PURPOSE:To provide the preparation method and the preparation apparatus, of a medicine or the like, in which the validation of a valve assembled in an apparatus or a plant is performed in the preparation operation of the medicine or the like and which prepares a normal medicine or the like. CONSTITUTION:Valves 6, 7 which are installed in a preparation line 1 and a cleaning line 2 are closed, and a space A which has been sealed airtightly by the valves is formed. Then, a valve 8 is closed, and the air is supplied to an air supply line 5 via a valve 9 which has been opened. Then, the valve 9 is closed, and a space B which is formed by means of the valves 6, 8, 9 is formed as a space whose pressure is higher than that of the space A. For a prescribed time, the pressure of the spaces A, B is measured by a pressure gage 10, and a change in the pressure is recorded by a recorder 12 via a cable 11. An inspection process which is described above is incorporated into an apparatus having a line, the change in the pressure is recorded, the validation materials of the valve 6 as an object are obtained by using a sheet of recording paper in which the pressure of the spaces A, B is not changed.

18. 发明专利

JPH06181748A PACKED BED SYSTEM CULTURE AND APPARATUS THEREFOR 失效
公开(公告)号：JPH06181748A
公开(公告)日：1994-07-05
申请号：JP33568692
申请日：1992-12-16
申请人： HITACHI LTD
发明人： YAMAGUCHI YOSHIHISA , MURAKAMI SEI , NAKANO TAKAMORI
IPC分类号： C12M3/00 , C12N5/02 , C12N5/07 , C12N5/071 , C12N5/06
摘要： PURPOSE:To provide an apparatus for culture, packed with carriers that the respective carriers may be held leaving a suitable space and exhibiting a high reaction (production) efficiency. CONSTITUTION:A stereoscopic structure 21 is designed so as to support carriers 20 in a packed bed 2 or the carriers 20 themselves are designed so as to have plural depression parts on the outer surface. Thereby, a 1 to 5mm space is left between the respective carriers 20 so as to form a passage 22 vertically passing through the packed bed 2. A passage for supplying a culture medium into the packed bed can be secured and a fresh culture medium can uniformly be supplied to the whole packed bed. An apparatus for culture, therefore, capable of maintaining the activity of the cells over the whole packed bed and of being readily scaled up with a higher efficiency can be obtained.

19. 发明专利

JPH06181737A CULTURE APPARATUS 失效
公开(公告)号：JPH06181737A
公开(公告)日：1994-07-05
申请号：JP33836492
申请日：1992-12-18
申请人： HITACHI LTD
发明人： YAMAGUCHI SHUJI , MURAKAMI SEI , NAKANO TAKAMORI
IPC分类号： C12M1/00 , C12M1/12
摘要： PURPOSE:To determine the sterilization effect at various parts of a culture apparatus by filling a suspension of indicator microorganism in the apparatus, sterilizing with high-pressure steam, recovering the proliferation medium of the indicator microorganism from each part and determining the growth of bacteria after cultivation. CONSTITUTION:A bacterial strain having strong resistance to high-pressure steam sterilization treatment and free from pathogenicity (e.g. thermostable sporangium) is used as an indicator microorganism and filled together with a culture liquid into a culture apparatus through indicator microorganism filling pipes 14-17. The indicator microorganism is distributed throughout the apparatus by extracting the culture liquid through indicator microorganism recovery pipes 18-21 connected to drain exhaustion ports 10-13. High-pressure steam is blown into the apparatus through steam inlet pipes 6-9 to sterilize the apparatus. The culture liquid is filled into the apparatus through the pipes 14-17, the system is maintained to the state for a prescribed period, the culture liquid is recovered from the system through the pipes 18-21 and the growth of bacteria (or clouding of the medium) is determined by culturing the liquid.

20. 发明专利

JPH0549483A METHOD FOR IRRADIATING LIVING CELL WITH LASER 失效
公开(公告)号：JPH0549483A
公开(公告)日：1993-03-02
申请号：JP19915691
申请日：1991-08-08
申请人： HITACHI LTD
发明人： YAMAGUCHI YOSHIHISA , NAKANO TAKAMORI , MURAKAMI SEI
IPC分类号： C12M1/00 , C12M1/42 , C12N1/00 , C12N5/10 , C12N13/00 , C12N15/09
摘要： PURPOSE:To efficiently transferring a substance into a cell by retaining a mixture consisting of a substance to be transferred into a cell, living cell and buffer solution at low temperature and irradiating the cell with laser beam through a objective lens of a microscope. CONSTITUTION:A vessel in which a mixture 9 consisting of a substance to be transferred, living cell 4 and buffer solution is charged is put on a table under which a cooling water 7 is circulated and laser beams are irradiated through an objective lens 2 of a microscope to the living cell 4 to carry out the objective transfer. Furthermore, the laser irradiation is preferably carried out in a room controlled at the required temperature.

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