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    • 13. 发明公开
    • SEKRETIONSOPTIMIERTER MIKROORGANISMUS
    • EP2291535A1
    • 2011-03-09
    • EP09757398.4
    • 2009-05-20
    • Henkel AG & Co. KGaA
    • BONGAERTS, JohannesEVERS, StefanFOH, KerstinMAURER, Karl-Heinz
    • C12P21/02C12N9/04C12N15/62C12N15/76
    • C12P21/02C12N9/00C12N9/0006C12N15/76
    • Proteins comprising a cofactor can be secreted in a microorganism belonging to the genus Streptomyces in an improved manner if the microorganism includes a nucleic acid sequence that is not naturally present in the same and comprises at least the following sequence sections: a) a nucleic acid sequence coding for a protein containing a cofactor, and b) a nucleic acid sequence that is at least 20% identical with the sequence given in SEQ ID NO. 1, or at least 20% identical with the sequence given in SEQ ID NO. 3, or a nucleic acid sequence that is structurally homologous to at least one of said sequences, wherein the amino acid sequence coded by the nucleic acid sequence b) functionally cooperates with the amino acid sequence coded by the nucleic acid sequence a) such that at least the amino acid sequence coded by the nucleic acid sequence a) is secreted by the microorganism.
    • 如果微生物包含非天然存在于其中的核酸序列并且至少包含以下序列部分,则包含辅因子的蛋白质可以以改进的方式分泌到属于链霉菌属的微生物中:a)核酸序列 编码含有辅因子的蛋白质,和b)与SEQ ID NO.1中给出的序列至少20%相同的核酸序列。 1或与SEQ ID NO.1中给出的序列至少20%相同。 3或与至少一个所述序列在结构上同源的核酸序列,其中由核酸序列b)编码的氨基酸序列在功能上与由核酸序列a)编码的氨基酸序列协作,使得在 至少由核酸序列a)编码的氨基酸序列由微生物分泌。