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    • 17. 发明申请
    • MULTI-MODALITY CONTRAST AND BRIGHTFIELD CONTEXT RENDERING FOR ENHANCED PATHOLOGY DETERMINATION AND MULTI-ANALYTE DETECTION IN TISSUE
    • 多模式对比度和亮度对比度增强,用于增强组织病理学确定和组织中的多分析检测
    • US20120200694A1
    • 2012-08-09
    • US13499959
    • 2010-10-07
    • Karl GarshaGary PestanoMichael OtterAlexandra Dea NagyRay B. NaglePhillip MillerJan FroehlichWilliam Day
    • Karl GarshaGary PestanoMichael OtterAlexandra Dea NagyRay B. NaglePhillip MillerJan FroehlichWilliam Day
    • G06K9/36H04N7/18
    • G01N21/6456
    • Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation. Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry.
    • 可以使用多重模态对比来产生可以组合和渲染的图像,以产生类似于在透射白光照明下观察到的波长吸收染色产生的图像的图像。 使用其他补充对比模式获得的图像可以使用基于用于显示相同解剖结构和组织化学的经典对比度方法的工程配色方案来呈现,从而提供与医学培训和经验相关的图像。 将衍生自折射率和荧光DAPI复染图像的暗视野对比图像组合以产生与用于病理解释的常规H&E染色获得的图像相似的图像。 这种多模式图像数据可以流式传输用于组织学样本的实时导航,并且可以与遗传DNA探针(FISH),mRNA表达位点(mRNA-ISH)和定位于其上的免疫组织化学(IHC)探针的分子定位相结合 相同的组织切片,用于评估和绘制用于成像质谱法制备的组织切片。