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11. 发明授权

EP3253867B1 POLYMERASE VARIANTS 有权
公开(公告)号：EP3253867B1
公开(公告)日：2020-05-06
申请号：EP16703461.0
申请日：2016-02-01
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： AYER, Aruna , BIBILLO, Arkadiusz , ECKERT, Barbara , SUKO, Shawn , ARNOLD, Cleoma Renetta , SCHWAB, Charles Wayan , THAI, Eileen , LEDERMAN, Ilya , MCGAW, Colin Alexander , SCHULTZ, Tyler O'Brien , BOENITZ-DULAT, Mara , WOERSDOERFER, Bigna , WUNDERLICH, David Daniel
IPC分类号： C12N9/00

12. 发明授权

EP1802748B1 THERMOSTABLE MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT GLUCOSE DEHYDROGENASE 有权转让
标题翻译： THERMO稳定的突变体吡咯CHINON依赖葡萄糖脱氢酶
公开(公告)号：EP1802748B1
公开(公告)日：2011-06-01
申请号：EP05792997.8
申请日：2005-10-14
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： BOENITZ-DULAT, Mara , KRATZSCH, Peter , SCHMUCK, Rainer
IPC分类号： C12N9/04 , C12N15/53 , C12Q1/32
CPC分类号： C12N9/0006
摘要： The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these sGDH mutants, particularly for determining the concentration of glucose in a sample.

13. 发明公开

EP3516053A2 POL6 POLYMERASE VARIANTS 审中-公开
公开(公告)号：EP3516053A2
公开(公告)日：2019-07-31
申请号：EP17781021.5
申请日：2017-09-20
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： BOENITZ-DULAT, Mara , SUKO, Shawn , AYER, Aruna , WOERSDOERFER, Bigna , ECKERT, Barbara , SARVABHOWMAN, Preethi , SCHWAB, Charles Wayan , WUNDERLICH, David , CLEOMA, Arnold , LEDERMAN, Ilya , THAI, Eileen , MCGAW, Colin , AMATO, Evan
IPC分类号： C12N9/12

14. 发明公开

EP3394252A1 MUTANT 3-HYDROXYBUTYRATE DEHYDROGENASE FROM ALCALIGENES FAECALIS AS WELL AS METHODS AND USES INVOLVING THE SAME 有权
公开(公告)号：EP3394252A1
公开(公告)日：2018-10-31
申请号：EP16826037.0
申请日：2016-12-21
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH , Roche Diabetes Care GmbH
发明人： KRATZSCH, Peter , BOENITZ-DULAT, Mara , BECK, Daniela , STREIDL, Thomas , HUNT DUVALL, Stacy
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , C12Q1/32 , C12Q1/54 , C12Y101/0103
摘要： The present disclosure relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell having the mutant 3-HBDH or the nucleic acid, and/or a method of determining the amount or concentration of 3-hydroxybutyrate in a sample. Also disclosed is the use of the mutant 3-HBDH for determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.

15. 发明授权

EP3414325B1 MUTANT 3-HYDROXYBUTYRATE DEHYDROGENASE FROM RHODOBACTER SPHAEROIDES AS WELL AS METHODS AND USES INVOLVING THE SAME 有权
公开(公告)号：EP3414325B1
公开(公告)日：2020-01-29
申请号：EP17704245.4
申请日：2017-02-09
申请人： Roche Diabetes Care GmbH , Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： BECK, Daniela , BOENITZ-DULAT, Mara , KRATZSCH, Peter , STREIDL, Thomas , HORN, Carina
IPC分类号： C12P7/18 , C12N9/04 , C12Q1/32

16. 发明公开

EP2010649A1 IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE 有权转让
标题翻译： VERBESSERTE MUTANTE MORANTEN PYRROLOCHINOLIN-CHINON-ABHÄNGIGERLÖSLICHERGLUCOSE-DEHYDROGENASE
公开(公告)号：EP2010649A1
公开(公告)日：2009-01-07
申请号：EP07724148.7
申请日：2007-04-11
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： BOENITZ-DULAT, Mara , BECK, Daniela , KRATZSCH, Peter , SCHMUCK, Rainer , VON DER ELTZ, Herbert
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
摘要： The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译：与麦芽糖相比，PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC 1.1.5.2）的突变体具有改善的葡萄糖特异性，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体还包含至少一个用于改善突变体的稳定性的突变和用于改善突变体对葡萄糖的亲和力的一个或多个突变，和/或用于进一步提高突变体的特异性的一个或多个突变用于与麦芽糖相比的葡萄糖，并且其中位置348对应于从A.calcoaceticus s-GDH野生型序列已知的氨基酸位置。还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。

17. 发明公开

EP4222257A1 BACTERIAL PILUS PROTEIN COMPLEX FIMGT-DSF STABILIZED PROTEIN COMPLEXES FOR PRODUCING FILAMENTOUS PHAGES 审中-实审
公开(公告)号：EP4222257A1
公开(公告)日：2023-08-09
申请号：EP21786129.3
申请日：2021-09-28
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： BOENITZ-DULAT, Mara , SCHRAEML, Michael , WOERSDOERFER, Bigna
IPC分类号： C12N15/10 , C07K14/245

18. 发明申请

WO2017134190A1 THIOL-BASED DEEP EUTECTIC SOLVENT 审中-公开
标题翻译：基于硫醇的深共晶溶剂
公开(公告)号：WO2017134190A1
公开(公告)日：2017-08-10
申请号：PCT/EP2017/052318
申请日：2017-02-03
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： BOENITZ-DULAT, Mara , SCHATTE, Martin
IPC分类号： C12P21/02 , C07C215/90 , C07C323/12 , C07C323/66
CPC分类号： C07C323/66 , C07C215/40 , C07C323/12 , C12P21/02
摘要： Herein is reported a deep eutectic solvent consisting of (2-hydroxyethyl) trimethyl ammonium chloride and dithiothreitol in a molar ratio of from 1:2 to 1:3 and of from 0 % to 10 % co-solvent.
摘要翻译：这里报道了由（2-羟乙基）三甲基氯化铵和二硫苏糖醇以1:2至1:3的摩尔比和从0％至10％的共沸溶剂组成的深共晶溶剂，溶剂

19. 发明申请

WO2019101722A1 PROLINE HYDROXYLASES AS WELL AS USES, METHODS AND PRODUCTS INVOLVING THE SAME 审中-公开
公开(公告)号：WO2019101722A1
公开(公告)日：2019-05-31
申请号：PCT/EP2018/081889
申请日：2018-11-20
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： BOENITZ-DULAT, Mara , IDING, Hans , WETZL, Dennis , HINZMANN, Alessa
IPC分类号： C12N9/02
摘要： The present invention relates to the use of a protein as a hydroxylase, a host cell comprising the protein, the use of the protein or host cell in the production of hydroxy-pipecolic acid (HPA), a method for production and purification of HPA, methods of producing the protein, a mutant protein and a nucleic acid encoding the mutant protein and a vector comprising a nucleic acid encoding the protein.

20. 发明申请

WO2017050874A1 PROCESS FOR PRODUCING THIOESTERS EMPLOYING A SORTASE A 审中-公开
标题翻译：生产使用SORTASE的硫酸酯的方法A
公开(公告)号：WO2017050874A1
公开(公告)日：2017-03-30
申请号：PCT/EP2016/072512
申请日：2016-09-22
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： SCHATTE, Martin , BOENITZ-DULAT, Mara , BERGMANN, Frank
IPC分类号： C12P11/00 , C12P21/02
CPC分类号： C12P11/00 , A61K47/65 , C12P21/02
摘要： Herein is reported a method for the enzymatic production of a thioester comprising incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue), ii) a second polypeptide that has at its N-terminus a cysteine amino acid residue or is a cysteinyl compound, and iii) a third polypeptide with sortase A activity.
摘要翻译：本文报道了一种用于酶促生产硫酯的方法，包括孵育i）包含氨基酸序列LPXTG（SEQ ID NO：01，其中X可以是任何氨基酸残基）的第一多肽，ii）具有其N末端是半胱氨酸氨基酸残基或是半胱氨酰化合物，和iii）具有分选酶A活性的第三种多肽。

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