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    • 12. 发明授权
    • Methods for cultivating cells, propagating and purifying viruses
    • 培养细胞,增殖和纯化病毒的方法
    • US09085753B2
    • 2015-07-21
    • US13473494
    • 2012-05-16
    • Jonathan LiuMark ThompsonLuis J. MarangaFloro CataniagSimon S. Hsu
    • Jonathan LiuMark ThompsonLuis J. MarangaFloro CataniagSimon S. Hsu
    • C12N7/02A61K39/145
    • C12N7/02A61K39/12A61K39/145C12N7/00C12N2760/16051C12N2760/16134C12N2760/16151C12N2760/16234C12N2760/16251
    • The present invention provides novel serum-free cell culture medium and methods for cultivating MDCK cells. In particular, non-tumorigenic MDCK cells. The present invention also provides methods for producing influenza viruses (e.g., particularly cold-adapted, and/or temperature sensitive, and/or attenuated influenza viruses) that eliminate the need for a cell culture medium exchange step. The novel medium and methods are useful to grow influenza viruses, in cell culture to high titer. The present invention further provides purification methods for purifying influenza viruses with high overall recovery of live virus and result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.
    • 本发明提供新型的无血清细胞培养基和培养MDCK细胞的方法。 特别是非致瘤性MDCK细胞。 本发明还提供了消除对细胞培养基交换步骤的需要的用于生产流感病毒(例如,特别冷适应的和/或温度敏感的和/或减毒的流感病毒)的方法。 新型培养基和方法可用于在细胞培养物中高效滴加流感病毒。 本发明还提供了用于纯化具有高活性病毒总体回收率的流感病毒的纯化方法,并导致宿主细胞DNA(HCD),宿主细胞蛋白(HCP)和非特异性内切核酸酶(例如,Benzonase)的水平,其低于 监管机构要求的规格。 免疫原性组合物可用于主动免疫受试者或产生用于多种用途的抗体,包括被动免疫和诊断性免疫测定。
    • 13. 发明申请
    • MULTI-USE TOOL TABLES
    • 多用工具表
    • US20140332657A1
    • 2014-11-13
    • US14444662
    • 2014-07-28
    • Mark Thompson
    • Mark Thompson
    • B25H1/14B25H1/04B25H1/02
    • B25H1/14B25H1/02B25H1/04
    • Multi-use tool tables configured to support table tools and allow selective rotation of the table tools between an upright position and an inverted position are described in the present application. The multi-use tool tables include: one or more rotatable plates having a first surface for receiving a first tool and a second opposing surface for receiving a second tool, a perimeter edge of the one or more rotatable plates being attached to a rotatable shaft; a column with a first attached to a support mechanism and a second attached to a sleeve, the sleeve being configured to receive the rotatable shaft; and a stop mechanism configured to selectively resist movement of the rotatable shaft within the sleeve. The one or more rotatable plates are rotatable between a first surface upright/second surface inverted position and a second surface upright/first surface inverted position.
    • 在本申请中描述了被配置为支撑工作台工具并允许桌子工具在直立位置和倒置位置之间的选择性旋转的多用工具台。 所述多用途工具台包括:一个或多个可旋转板,其具有用于接收第一工具的第一表面和用于接收第二工具的第二相对表面,所述一个或多个可旋转板的周边边缘附接到可旋转轴; 柱,其首先附接到支撑机构,第二连接到套筒,所述套筒构造成容纳所述可旋转轴; 以及停止机构,其构造成选择性地阻止所述可旋转轴在所述套筒内的移动。 一个或多个可旋转板可在第一表面竖立/第二表面倒置位置和第二表面竖立/第一表面倒置位置之间旋转。
    • 16. 发明申请
    • COLLAPSIBLE, PORTABLE STRUCTURE
    • 可拆卸,便利的结构
    • US20130037069A1
    • 2013-02-14
    • US13325188
    • 2011-12-14
    • Kerry PritchardMark ThompsonAnthony Brereton
    • Kerry PritchardMark ThompsonAnthony Brereton
    • E04H15/48
    • A63H33/008E04H15/006E04H15/44
    • A collapsible portable structure which may provide a play environment for children has side walling defining an upright corner region with a free side edge and a canopy which straddles the corner region. The canopy may provide a roof-like cover or at least a partial enclosure. The side walling is formed by at least one continuous loop of a flexible coilable rod supporting a flexible fabric panel. Thus there may be one curved or angular panel, or two adjoining panels or panel sections. The canopy is of flexile fabric and is attached to the fabric of the side walling at a location spaced inwardly from the free edge of the side walling. The canopy is supported separately from the side walling panels by at least one separate pole. A ground sheet may optionally be provided extending across the corner region between the lower edges of the side walling.
    • 可以为儿童提供游戏环境的可折叠便携式结构具有侧壁,其限定具有自由侧边缘的直立角区域和跨越拐角区域的冠层。 顶篷可以提供屋顶状的盖子或至少一个部分外壳。 侧壁由至少一个支撑柔性织物面板的柔性可卷绕杆的连续环形成。 因此,可以存在一个弯曲或有角度的面板,或者两个邻接的面板或面板部分。 顶篷是柔性织物,并且在与侧壁的自由边缘间隔开的位置附接到侧壁织物。 顶篷通过至少一个单独的杆与侧壁板分开支撑。 可以可选地设置接地片,跨越侧壁的下边缘之间的拐角区域延伸。