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11. 发明授权

US10378019B2 Premethylation of DNA for high efficiency transformation of cyanobacteria 有权
公开(公告)号：US10378019B2
公开(公告)日：2019-08-13
申请号：US14842671
申请日：2015-09-01
申请人： Bo Wang , Weiwen Zhang , Deirdre Meldrum
发明人： Bo Wang , Weiwen Zhang , Deirdre Meldrum
IPC分类号： C12N15/74
摘要： Methods of pre-methylation of foreign DNA to improve genetic transformation in cyanobacterium. Two Type II methyltransferase-encoding genes, i.e., M (sll0729) and C (slr0214), were cloned from the chromosome of Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803) and expressed in E. coli that harbors the integrative plasmid pBS-SPtK or pJU105. After pre-methylation in E. coli, the integrative plasmids were extracted and used for transformation of Synechocystis 6803. The results showed that expression of slr0214 in the integrative-plasmid-harboring E. coli cells before DNA preparation resulted in orders of magnitude higher efficiency in the following integrative transformation of Synechocystis 6803.

12. 发明申请

US20160202247A1 PHOTO-PATTERNABLE OPTICAL LUMINESCENCE DUAL SENSORS AND METHODS OF PREPARING AND USING THEM 审中-公开
标题翻译：照片型光学发光双传感器及其制作和使用方法
公开(公告)号：US20160202247A1
公开(公告)日：2016-07-14
申请号：US14996170
申请日：2016-01-14
申请人： Yanqing Tian , Hong Wang , Ganquan Song , Fengyu Su , Liqiang Zhang , Deirdre Meldrum , Laimonas Kelbauskas , Benjamin Ueberroth
发明人： Yanqing Tian , Hong Wang , Ganquan Song , Fengyu Su , Liqiang Zhang , Deirdre Meldrum , Laimonas Kelbauskas , Benjamin Ueberroth
IPC分类号： G01N33/52 , G01N21/64
CPC分类号： G01N33/84 , G01N31/221 , G01N31/225
摘要： The present disclosure relates to an optical luminescence dual sensor comprising a polymerized form of a probe for sensing pH; a polymerized form of a probe for sensing oxygen; a polymerized form of an internal reference probe; and a matrix. The present disclosure also relates to methods of preparing an optical luminescence dual sensor and methods of using them.
摘要翻译：本公开涉及一种光学发光双传感器，其包括用于感测pH的探针的聚合形式; 用于感测氧的探针的聚合形式; 内部参考探针的聚合形式; 和矩阵。本公开还涉及制备光学发光双传感器的方法及其使用方法。

13. 发明申请

US20190177784A1 METHOD FOR DETECTING NUCLEOTIDE POLYMORPHISMS 审中-公开
公开(公告)号：US20190177784A1
公开(公告)日：2019-06-13
申请号：US16323397
申请日：2017-08-07
申请人： Rhett Martineau , Deirdre Meldrum
发明人： Rhett Martineau , Deirdre Meldrum
IPC分类号： C12Q1/6858 , C12Q1/6883
摘要： Methods for detecting single nucleotide polymorphisms in nucleotide sequences using LAMP reactions are provided herein. Generally, two sets of LAMP primers, a wild-type primer that matches expected DNA sequences and an SNP primer that matches the expected SNP DNA are provided. One method includes providing the wild-type primer and the SNP primer in separate wells of a multi-well microfluidic array device, adding the sample nucleotide sequence into the wells seeded with the primers, and initiating LAMP reactions within the wells. The method includes observing the reaction differential between the primers and determining the status of the DNA with regard to that particular SNP. A second method includes providing the primers with tags in a mixture, adding the sample nucleotide sequence to the mixture, and initiating LAMP reactions. The method includes providing a different visual indication when the wild-type primer reacts with the sample nucleotide sequence versus when the SNP primer reacts with the sample nucleotide sequence, and determining the status of the DNA with regard to that particular SNP.

14. 发明申请

US20140200319A1 FLUORESCENT POTASSIUM ION SENSORS 有权
标题翻译：荧光钾离子传感器
公开(公告)号：US20140200319A1
公开(公告)日：2014-07-17
申请号：US13985260
申请日：2012-02-13
申请人： Yanqing Tian , Deirdre Meldrum , Xianfeng Zhou , Fengyu Su , Roger H. Johnson , Cody Youngbull
发明人： Yanqing Tian , Deirdre Meldrum , Xianfeng Zhou , Fengyu Su , Roger H. Johnson , Cody Youngbull
IPC分类号： C08F226/06 , C08F230/04 , C07D498/08
CPC分类号： C08F226/06 , C07D498/08 , C07D519/00 , C07F15/0093 , C08F230/04 , C08G2261/226 , C08G2261/3223 , G01N33/84
摘要： This disclosure relates generally to potassium ion sensors and monomers derived from such sensors. The disclosure also provides for polymers (e.g., random copolymers, nanoparticles, polymer thin films, and sensors) having polymerized monomeric potassium ion sensors as described herein. These compounds and polymers are useful for measuring intracellular and extracellular potassium ion concentrations.
摘要翻译：本公开一般涉及衍生自这种传感器的钾离子传感器和单体。本公开还提供了具有如本文所述的聚合的单体钾离子传感器的聚合物（例如，无规共聚物，纳米颗粒，聚合物薄膜和传感器）。这些化合物和聚合物可用于测量细胞内和细胞外钾离子浓度。

15. 发明授权

US07910934B2 Self-assembled heterogeneous integrated optical analysis system 有权
标题翻译：自组装异构集成光学分析系统
公开(公告)号：US07910934B2
公开(公告)日：2011-03-22
申请号：US12497518
申请日：2009-07-02
申请人： Samuel Kim , Babak Amirparviz , Deirdre Meldrum , Ehsan Saeedi
发明人： Samuel Kim , Babak Amirparviz , Deirdre Meldrum , Ehsan Saeedi
IPC分类号： H01L27/15 , H01L29/16 , H01L31/12 , H01L33/00
CPC分类号： H01L25/167 , B01L3/5027 , G01N21/253 , G01N21/6454 , H01L24/95 , H01L25/042 , H01L31/18 , H01L2924/09701 , H01L2924/12041 , H01L2924/12043 , H01L2924/00
摘要： Optical analysis system fluidically self-assembled using shape-coded freestanding optoelectronic components and a template having shape-coded recessed binding sites connected by an embedded interconnect network. Also includes methods of manufacture and use for optical analyses.
摘要翻译：光学分析系统使用形状编码的独立光电子元件进行流体自组装，以及具有通过嵌入式互连网络连接的形状编码的嵌入式结合位点的模板。还包括制造和使用光学分析的方法。

16. 发明申请

US20100096640A1 SELF-ASSEMBLED HETEROGENEOUS INTEGRATED OPTICAL ANALYSIS SYSTEM 有权
标题翻译：自组装异构一体化光学分析系统
公开(公告)号：US20100096640A1
公开(公告)日：2010-04-22
申请号：US12497518
申请日：2009-07-02
申请人： Samuel Kim , Babak Amirparviz , Deirdre Meldrum , Ehsan Saeedi
发明人： Samuel Kim , Babak Amirparviz , Deirdre Meldrum , Ehsan Saeedi
IPC分类号： H01L33/00 , H01L21/50
CPC分类号： H01L25/167 , B01L3/5027 , G01N21/253 , G01N21/6454 , H01L24/95 , H01L25/042 , H01L31/18 , H01L2924/09701 , H01L2924/12041 , H01L2924/12043 , H01L2924/00
摘要： Optical analysis system fluidically self-assembled using shape-coded freestanding optoelectronic components and a template having shape-coded recessed binding sites connected by an embedded interconnect network. Also includes methods of manufacture and use for optical analyses.
摘要翻译：光学分析系统使用形状编码的独立光电子元件进行流体自组装，以及具有通过嵌入式互连网络连接的形状编码的嵌入式结合位点的模板。还包括制造和使用光学分析的方法。

17. 发明授权

US06218193B1 Precision small volume fluid processing apparatus and method 失效
标题翻译：精密小体积流体处理设备及方法
公开(公告)号：US06218193B1
公开(公告)日：2001-04-17
申请号：US09107242
申请日：1998-06-30
申请人： Ray Kraft , Neal A. Friedman , Deirdre Meldrum , Ronald Seubert
发明人： Ray Kraft , Neal A. Friedman , Deirdre Meldrum , Ronald Seubert
IPC分类号： B01L302
CPC分类号： B01F13/0071 , B01F11/0071 , B01F13/0059 , B01F13/0084 , B01F15/0201 , B01F15/0232 , B01F2215/0409 , B01L3/02 , B01L3/022 , B01L3/0262 , B01L3/0268 , B01L3/06 , B01L9/065 , B01L2200/0605 , B01L2200/143 , B01L2300/0838 , B01L2300/18 , B01L2400/0478 , G01N2035/00237 , Y10T436/118339 , Y10T436/119163 , Y10T436/25625 , Y10T436/2575
摘要： A high precision, small volume fluid processing system employs open ended capillary tubes to meter, aliquot and mix small volumes of sample fluid and reagents. The system has an automatic mechanism for moving the capillary tubes as well as automated sub-systems for incubating and mixing fluids within the capillary tubes.
摘要翻译：高精度，小体积的流体处理系统采用开放式毛细管来计量，等分和混合少量的样品液和试剂。该系统具有用于移动毛细管的自动机构以及用于在毛细管内孵育和混合流体的自动子系统。

18. 发明申请

US20160060643A1 PREMETHYLATION OF DNA FOR HIGH EFFICIENCY TRANSFORMATION OF CYANOBACTERIA 审中-公开
标题翻译：用于高效转染细胞因子的DNA的预处理
公开(公告)号：US20160060643A1
公开(公告)日：2016-03-03
申请号：US14842671
申请日：2015-09-01
申请人： Bo Wang , Weiwen Zhang , Deirdre Meldrum
发明人： Bo Wang , Weiwen Zhang , Deirdre Meldrum
IPC分类号： C12N15/82
CPC分类号： C12N15/74
摘要： Methods of pre-methylation of foreign DNA to improve genetic transformation in cyanobacterium. Two Type II methyltransferase-encoding genes, i.e., M (sll0729) and C (slr0214), were cloned from the chromosome of Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803) and expressed in E. coli that harbors the integrative plasmid pBS-SPtK or pJU105. After pre-methylation in E. coli, the integrative plasmids were extracted and used for transformation of Synechocystis 6803. The results showed that expression of slr0214 in the integrative-plasmid-harboring E. coli cells before DNA preparation resulted in orders of magnitude higher efficiency in the following integrative transformation of Synechocystis 6803.
摘要翻译：外源DNA预甲基化方法可改善蓝细菌的遗传转化。从Synechocystis sp。的染色体上克隆了两个II型甲基转移酶编码基因，即M（sll0729）和C（slr0214）。 PCC 6803（以下称为集胞藻6803），并在含有整合质粒pBS-SPtK或pJU105的大肠杆菌中表达。在大肠杆菌预甲基化后，提取整合质粒并用于转化集胞藻6803。结果表明，在DNA制备前，含有整合质粒的大肠杆菌细胞中slr0214的表达导致了更高效率的数量级在下一代SynChocystis 6803的综合转化中。

19. 发明申请

US20150253333A1 CELLARIUM: GLUCOSE, PH, AND OXYGEN TRIPLE SENSOR 审中-公开
标题翻译：葡萄糖：葡萄糖，PH和氧三重传感器
公开(公告)号：US20150253333A1
公开(公告)日：2015-09-10
申请号：US14197422
申请日：2014-03-05
申请人： Yanqing Tian , Liqiang Zhang , Fengyu Su , Deirdre Meldrum , Sean Buizer
发明人： Yanqing Tian , Liqiang Zhang , Fengyu Su , Deirdre Meldrum , Sean Buizer
IPC分类号： G01N33/66 , G01N33/50
CPC分类号： G01N33/66 , G01N33/52 , G01N33/84
摘要： A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.
摘要翻译：三重传感器，用于同时测量葡萄糖，氧气和pH值。传感器组件处于薄膜状态，例如感测膜或膜，葡萄糖探针与光谱蓝色部分中的辐射相关，与红色光谱中的辐射相关的氧探针，以及pH探针 - 具有绿色部分的光谱。光学探针被化学接枝或固定在合适的聚合物基质中，减轻了探针从基体中的浸出，改善了薄膜感测的稳定性，并使得能够重复使用相同的传感膜。

20. 发明申请

US20070031819A1 Microfluidic systems for biological and molecular analysis and methods thereof 审中-公开
标题翻译：用于生物和分子分析的微流体系统及其方法
公开(公告)号：US20070031819A1
公开(公告)日：2007-02-08
申请号：US11412819
申请日：2006-04-26
申请人： John Koschwanez , Mark Holl , Robert Carlson , Michael McMurray , Daniel Gottschling , Deirdre Meldrum
发明人： John Koschwanez , Mark Holl , Robert Carlson , Michael McMurray , Daniel Gottschling , Deirdre Meldrum
IPC分类号： C12Q1/00 , G06K9/00 , C12M1/34
CPC分类号： G06K9/00127 , B01L3/502707 , B01L3/502761 , B01L2200/0668 , B01L2300/0877 , B01L2400/0487 , G01N33/54326 , G01N33/585
摘要： Disclosed herein are systems, components, devices and methods for automated yeast pedigree analysis. Systems, components, devices and methods for analyzing microorganisms, cells, particles and molecules are also disclosed.
摘要翻译：本文公开了用于自动化酵母谱系分析的系统，组件，装置和方法。还公开了用于分析微生物，细胞，颗粒和分子的系统，组件，装置和方法。

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