专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明申请

US20070003960A1 RNA sequence-specific mediators of RNA interference 审中-公开
标题翻译： RNA干扰的RNA序列特异性介质
公开(公告)号：US20070003960A1
公开(公告)日：2007-01-04
申请号：US11474738
申请日：2006-06-26
申请人： Thomas Tuschl , Phillip Zamore , Phillip Sharp , David Bartel
发明人： Thomas Tuschl , Phillip Zamore , Phillip Sharp , David Bartel
IPC分类号： C12Q1/68 , A01K67/033 , C12N1/21 , C12N5/06
CPC分类号： C12N15/113 , A01K67/0336 , A01K2207/05 , A01K2217/075 , A01K2227/703 , A01K2267/03 , A61K38/00 , C07H21/02 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12Q1/66 , C12N2310/3521
摘要： The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.
摘要翻译：本发明涉及果蝇体外系统，其用于证明dsRNA被加工成长度为21-23个核苷酸（nt）的RNA片段。此外，当这些21-23个片段被纯化并加回到果蝇提取物时，它们在不存在长dsRNA的情况下介导RNA干扰。因此，这些21-23个片段是RNA降解的序列特异性介质。必须在这21-23个片段中存在可能是其特异性长度的分子信号，以招募涉及RNAi的细胞因子。本发明包括这些21-23个片段及其用于特异性失活基因功能的用途。使用这些片段（或具有相同或相似性质的化学合成的寡核苷酸）使得能够靶向用于哺乳动物细胞降解的特异性mRNA，其中使用长dsRNA引发RNAi通常是不实际的，可能是因为有害影响干扰素反应。特定基因功能的特异性靶向在功能基因组和治疗应用中是有用的。

12. 发明申请

US20060283361A1 SAFE 有权
标题翻译：安全
公开(公告)号：US20060283361A1
公开(公告)日：2006-12-21
申请号：US11463473
申请日：2006-08-09
申请人： David Bartel , Melvin Keehart
发明人： David Bartel , Melvin Keehart
IPC分类号： E05D15/56 , E06B3/50
CPC分类号： E05D15/56 , E05D15/58 , E05G1/00 , E05G1/026 , E05Y2900/20 , E05Y2900/602 , E06B3/5045
摘要： A safe having a support assembly disposed in the interior of the safe. The door of the safe is coupled to the support assembly and is shiftable between a closed position wherein the door is received in an opening of the safe and an open position wherein the door is removed from the opening in the safe and disposed in the interior of the safe.
摘要翻译：具有设置在保险箱内部的支撑组件的保险箱。保险柜的门联接到支撑组件，并且可在关闭位置之间移动，其中门被容纳在保险箱的开口中并且打开位置，其中门从保险箱中的开口移除并且被设置在安全

13. 发明申请

US20050079614A1 RNAs able to modulate chromatin silencing 审中-公开
标题翻译： RNA能够调节染色质沉默
公开(公告)号：US20050079614A1
公开(公告)日：2005-04-14
申请号：US10895644
申请日：2004-07-21
申请人： Brenda Reinhart , David Bartel
发明人： Brenda Reinhart , David Bartel
IPC分类号： C12N15/113 , A61K48/00 , C12N15/85
CPC分类号： C12N15/113 , C12N2310/14 , C12N2330/10
摘要： This invention generally relates to nucleotide sequences and, in particular, to nucleotide sequences able to bind to or otherwise associate with DNA or chromatin, or otherwise modulate chromatin silencing. In certain embodiments, the nucleotide sequence may be present in (or encode for) a noncoding and/or nonexpressable RNA having less than 50 or 100 nucleotides, preferably about 20-30 nucleotides. In some cases, a precursor nucleic acid may be cleaved in some fashion to produce the nucleotide sequence. In one set of embodiments, the nucleotide sequences of the invention are not native to the cell, i.e., not normally present in the cell. In certain cases, the nucleotide sequence may be a 20-25 nucleotide RNA molecule that occurs naturally in other cells and/or in other organisms, or the nucleotide sequence may be an artificially generated nucleotide sequence, and in such cases, the nucleotide sequence is referred to herein as “heterochromatic small interfering RNA,” or “heterochromatic siRNA.” The nucleotide sequences may also be present within a nucleic-acid protein complex in certain embodiments. The nucleotide sequences of the invention may inhibit gene function, for example, by interacting with chromatin, and/or by transcriptionally inhibiting mRNA synthesis. In certain cases, the heterochromatic siRNA of the invention can further posttranscriptionally inhibit gene function, e.g., by binding to mRNA. In some cases, inhibition of the gene may be epigenetic or may otherwise be stable for relatively long periods of time, i.e., chromatin may be epigenetically altered through interaction with the nucleotide sequence, for example, through altered methylation of DNA or histones.
摘要翻译：本发明一般涉及核苷酸序列，尤其涉及能够结合或以其它方式结合DNA或染色质或以其他方式调节染色质沉默的核苷酸序列。在某些实施方案中，核苷酸序列可以存在于（或编码）具有小于50或100个核苷酸，优选约20-30个核苷酸的非编码和/或不可压缩的RNA。在一些情况下，前体核酸可以以某种方式切割以产生核苷酸序列。在一组实施方案中，本发明的核苷酸序列不是细胞天然的，即通常不存在于细胞中。在某些情况下，核苷酸序列可以是在其他细胞和/或其他生物体中天然存在的20-25个核苷酸的RNA分子，或者核苷酸序列可以是人造产生的核苷酸序列，在这种情况下，核苷酸序列是本文称为“异色小干扰RNA”或“异染色体siRNA”。在某些实施方案中，核苷酸序列也可存在于核酸蛋白复合物内。本发明的核苷酸序列例如可以通过与染色质相互作用和/或通过转录抑制mRNA合成来抑制基因功能。在某些情况下，本发明的异染色体siRNA可进一步转录后抑制基因功能，例如通过结合mRNA。在一些情况下，基因的抑制可以是表观遗传的，或者可能在相对长的时间段内可能是稳定的，即染色质可以通过与核苷酸序列的相互作用而被表观遗传改变，例如通过DNA或组蛋白的改变的甲基化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式