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11. 发明申请

WO2013010094A1 STACKED HERBICIDE TOLERANCE EVENT 8264.42.32.1, RELATED TRANSGENIC SOYBEAN LINES, AND DETECTION THEREOF 审中-公开
标题翻译：堆肥除草耐受性事件8264.42.32.1，相关转基因大豆品种及其检测
公开(公告)号：WO2013010094A1
公开(公告)日：2013-01-17
申请号：PCT/US2012/046706
申请日：2012-07-13
申请人： DOW AGROSCIENCES LLC , MS TECHNOLOGIES, LLC , HOFFMAN, Thomas , PARKHURST, Dawn Marie , ZHOU, Ning , PAREDDY, Dayakar , CUI, Yunxing Cory , BARD, Nathan , TOLEDO, Sandra Grace , BRADFISCH, Gregory Alan , HELD, Bruce , SEKAR, Vaithilingam , WANG, Yang , CLARK, Lauren , RUSSELL, Sean Michael , SMITH, Kelley Ann , WRIGHT, Terry R.
发明人： HOFFMAN, Thomas , PARKHURST, Dawn Marie , ZHOU, Ning , PAREDDY, Dayakar , CUI, Yunxing Cory , BARD, Nathan , TOLEDO, Sandra Grace , BRADFISCH, Gregory Alan , HELD, Bruce , SEKAR, Vaithilingam , WANG, Yang , CLARK, Lauren , RUSSELL, Sean Michael , SMITH, Kelley Ann , WRIGHT, Terry R.
IPC分类号： A01H5/00 , C12N5/04 , C12N5/10
CPC分类号： C12N15/8274 , A01H5/10 , C12N15/8275 , C12N15/8277 , C12Y114/11017 , C12Y203/01183 , C12Y205/01019
摘要： This invention relates to soybean event pDAB8264.42.32.1 and includes novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be "stacked" with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TAQMAN PCR assays for the detection of Event pDAB8264.42.32.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
摘要翻译：本发明涉及大豆事件pDAB8264.42.32.1，并且包括新型表达盒和包含赋予对草甘膦，芳氧基链烷酸酯和草铵膦除草剂的抗性的多种性状的转基因插入物。本发明还部分涉及控制抗性杂草，植物育种和除草剂耐受性植物的方法。在一些实施方案中，事件序列可以与其他性状“堆叠”，包括例如其它除草剂耐受性基因和/或昆虫抑制蛋白。本发明还部分涉及在大豆和相关植物材料中检测事件pDAB8264.42.32.1的终点TAQMAN PCR测定。一些实施方案可以进行植物材料的高通量接合度分析，并且可以使用其它实施方案来唯一地鉴定包含本发明事件的大豆品系的接合度和品种。还提供了用于进行这些测定的试剂盒和条件。

12. 发明申请

WO2013016520A1 SOYBEAN EVENT pDAB9582.814.19.1 DETECTION METHOD 审中-公开
标题翻译：大豆事件pDAB9582.814.19.1检测方法
公开(公告)号：WO2013016520A1
公开(公告)日：2013-01-31
申请号：PCT/US2012/048311
申请日：2012-07-26
申请人： DOW AGROSCIENCES LLC , CLARK, Lauren , SMITH, Kelley Ann , WANG, Yang , ZHOU, Ning
发明人： CLARK, Lauren , SMITH, Kelley Ann , WANG, Yang , ZHOU, Ning
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6895 , C12Q2600/16
摘要： Soybean Event pDAB9582.814.19.1 comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The invention provides PCR event detection methods.
摘要翻译：大豆事件pDAB9582.814.19.1包含编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及对作物保护和保护储存产品的有效方法。本发明提供PCR事件检测方法。

13. 发明申请

WO2012033808A2 METHODS FOR DETERMINING THE PRESENCE OR ZYGOSITY OF AAD-12 SOYBEAN EVENT 1606 审中-公开
标题翻译：确定AAD-12大豆事件1606的存在或正交性的方法
公开(公告)号：WO2012033808A2
公开(公告)日：2012-03-15
申请号：PCT/US2011/050644
申请日：2011-09-07
申请人： DOW AGROSCIENCES LLC , ZHOU, Ning , NOVAK, Steven, N. , CUI, Yunxing
发明人： ZHOU, Ning , NOVAK, Steven, N. , CUI, Yunxing
IPC分类号： C12Q1/68
CPC分类号： A01H1/04 , C12N15/8274
摘要： The subject invention provides assays for detecting the presence of soybean event pDAB4472-1606 (Event 1606). In some embodiments, the assays can be based on the DNA sequence of the recombinant construct, inserted into the soybean genome, and on the genomic sequences flanking the insertion site. The subject invention also relates in part to endpoint TaqMan PCR assays for the detection of Event 1606 that expresses AAD-12 in soybeans. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. These and other related procedures can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
摘要翻译：本发明提供了用于检测大豆事件pDAB4472-1606（事件1606）的存在的测定法。在一些实施方案中，测定可以基于插入大豆基因组中的重组构建体的DNA序列和插入位点侧翼的基因组序列。本发明还部分涉及用于检测在大豆中表达AAD-12的事件1606的终点TaqMan PCR测定。一些实施方案涉及能够进行高通量接合度分析的测定。这些和其他相关方法可用于唯一地鉴定包含本发明事件的大豆品系的接合度和品种。还提供了用于进行这些测定的试剂盒和条件。

14. 发明公开

EP2591023A2 DEGRADABLE SUPERABSORBENT POLYMERS 审中-公开
标题翻译：生物降解聚合物高吸水性
公开(公告)号：EP2591023A2
公开(公告)日：2013-05-15
申请号：EP11734229.5
申请日：2011-07-05
申请人： Reluceo Inc. , Scholten, Marc , Zhou, Ning
发明人： SELIFONOV, Sergey , SCHOLTEN, Marc , ZHOU, Ning
IPC分类号： C08F216/38 , C08F8/14
CPC分类号： C08G4/00 , A01N25/26 , B01J20/262 , C08F8/14 , C08F8/28 , C08F8/44 , C08F216/38 , C09D161/00 , Y10T428/2933 , Y10T428/2982 , Y10T428/2991 , Y10T428/31855 , Y10T442/20 , C08F216/06 , A01N25/10 , A01N25/34
摘要： The present disclosure relates to degradable superabsorbent materials based on acetals of glyoxylic acid and derivatives thereof with polyvinyl alcohol, and methods of making the polymers. The polymers are used to make superabsorbent particles, coatings, sheets, and fibers. Formulations and articles including the superabsorbent polymers, particles, coatings, sheets, and fibers are also disclosed.

15. 发明申请

WO2014093736A1 DNA DETECTION METHODS FOR SITE SPECIFIC NUCLEASE ACTIVITY 审中-公开
标题翻译：用于现场特异性核素活性的DNA检测方法
公开(公告)号：WO2014093736A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/074851
申请日：2013-12-13
申请人： DOW AGROSCIENCES LLC
发明人： SASTRY-DENT, Lakshmi , SIMPSON, Matthew , CAO, Zehui , CHEN, Wei , ZHOU, Ning , WEBB, Steven R.
IPC分类号： C40B40/08 , C12N5/00 , C12N5/02
CPC分类号： C12Q1/6858 , A01G24/00 , C12N5/00 , C12N15/1082 , C12N15/66 , C12N15/70 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B40/08
摘要： The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease
摘要翻译：本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测靶基因组基因座上的供体DNA多核苷酸插入。该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件

16. 发明申请

WO2014004458A2 INSECT RESISTANT AND HERBICIDE TOLERANT SOYBEAN EVENT PDAB9582.816.15.1 审中-公开
标题翻译：耐药和除草剂耐受性大豆事件PDAB9582.816.15.1
公开(公告)号：WO2014004458A2
公开(公告)日：2014-01-03
申请号：PCT/US2013/047539
申请日：2013-06-25
申请人： DOW AGROSCIENCES LLC
发明人： BARD, Nathan , BRADFISCH, Gregory, A. , CUI, Yunxing, Cory , DRIPPS, James, E. , HOFFMAN, Thomas , PAREDDY, Dayakar , PARKHURST, Dawn, M. , TOLEDO, Sandra, G. , WIGGINS, Barry , ZHOU, Ning , WOOSLEY, Aaron, T.
IPC分类号： A01H5/00
CPC分类号： A01N43/16 , A01H5/10 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Soybean event 9582.816.15.1 comprises genes encoding CrylF, CrylAc (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.
摘要翻译：大豆事件9582.816.15.1包含编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及用于作物保护和储存产品保护的方法。

17. 发明申请

WO2014004472A1 SOYBEAN EVENT PDAB9582.816.15.1 DETECTION METHOD 审中-公开
标题翻译：大豆事件PDAB9582.816.15.1检测方法
公开(公告)号：WO2014004472A1
公开(公告)日：2014-01-03
申请号：PCT/US2013/047566
申请日：2013-06-25
申请人： DOW AGROSCIENCES LLC
发明人： CLARK, Lauren , SMITH, Kelley, Ann , WANG, Yang , ZHOU, Ning
IPC分类号： C12N15/11
CPC分类号： C12Q1/6895 , C12N15/8277 , C12N15/8286 , C12Q2600/13 , C12Q2600/156 , Y02A40/162
摘要： Soybean Event pDAB9582.816.15.1 comprises gene expression cassettes which contain genes encoding CrylF, CrylAc (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The disclosure provides polynucleotiderelated event detection methods. The present disclosure relates to a method for detecting a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated Soybean Event pDAB9582.816.15,1. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome. SEQ ID NO: 1 and SEQ ID N0:2 are diagnostic for Soybean Event pDAB9582.816.15.1.
摘要翻译：大豆事件pDAB9582.816.15.1包含基因表达盒，其含有编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及用于作物保护和储存产品保护的方法。本公开提供了多核苷酸相关事件检测方法。本公开涉及一种用于检测新的耐昆虫和除草剂耐受转基因大豆转化事件的方法，其命名为大豆事件pDAB9582.816.15,1。含有该事件的大豆植物的DNA包括本文所述的连接/侧翼序列，其表征插入的DNA在大豆基因组内的位置。 SEQ ID NO：1和SEQ ID N0：2是大豆事件pDAB9582.816.15.1的诊断。

18. 发明申请

WO2013078318A1 HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS 审中-公开
标题翻译：转基因边界的高通量分析
公开(公告)号：WO2013078318A1
公开(公告)日：2013-05-30
申请号：PCT/US2012/066267
申请日：2012-11-21
申请人： DOW AGROSCIENCES LLC
发明人： CAO, Zehui , NOVAK, Stephen , SASTRY-DENT, Lakshmi , ZHOU, Ning
IPC分类号： C40B20/00 , C40B30/00 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6895
摘要： A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing.
摘要翻译：在其中掺入转基因的染色体DNA中分析衍生自与转基因相邻的染色体的DNA侧翼区的方法。其中DNA侧翼区的特征在于用限制酶分离和消化基因组DNA，将双链衔接子连接到分离和消化的基因组DNA上，衔接子连接的基因组DNA的引物延伸反应和通过链霉亲和素 - 生物素相互作用引物延伸反应产物。通过随后的PCR扩增反应和DNA测序进一步表征DNA侧翼区。

19. 发明申请

WO2010075330A1 KETAL AMIDE COMPOUNDS, METHODS OF MAKING, AND APPLICATIONS 审中-公开
标题翻译：酮类化合物，制备方法和应用
公开(公告)号：WO2010075330A1
公开(公告)日：2010-07-01
申请号：PCT/US2009/069101
申请日：2009-12-22
申请人： SEGETIS, INC. , SELIFONOV, Sergey , GOETZ, Adam, E. , SCHOLTEN, Marc , ZHOU, Ning
发明人： SELIFONOV, Sergey , GOETZ, Adam, E. , SCHOLTEN, Marc , ZHOU, Ning
IPC分类号： C07D317/30
CPC分类号： C07D319/06 , C07D317/30
摘要： Disclosed herein are ketal amide compounds. The ketal amide compounds are synthesized by the reaction of ketal acids or ketal esters with amine functional compounds. Also disclosed are methodologies useful to make the ketal amide compounds. Also disclosed herein are formulations and articles containing the ketal amide compounds.
摘要翻译：本文公开的是缩酮酰胺化合物。缩酮酰胺化合物通过缩酮酸或缩酮与胺官能化合物的反应合成。还公开了可用于制备缩酮酰胺化合物的方法。本文还公开了含有缩酮酰胺化合物的制剂和制品。

20. 发明申请

WO2007149320A1 PRODUCTION OF STABILIZED WHOLE GRAIN FLOUR AND PRODUCTS THEREOF 审中-公开
标题翻译：生产稳定的全谷物粉及其制品
公开(公告)号：WO2007149320A1
公开(公告)日：2007-12-27
申请号：PCT/US2007/014053
申请日：2007-06-15
申请人： KRAFT FOODS HOLDINGS, INC. , HAYNES, Lynn, C. , LEVINE, Harry, Ira , SLADE, Louise , ZHOU, Ning , MANN, James , GANNON, Diane , HOWEY, Edward, D. , MIHALOS, Mihaelos, N. , EPPERSON, C., William , GABRIEL, Sarwat , CASSONE, Domenico , ZIMERI, Jeanny, E.
发明人： HAYNES, Lynn, C. , LEVINE, Harry, Ira , SLADE, Louise , ZHOU, Ning , MANN, James , GANNON, Diane , HOWEY, Edward, D. , MIHALOS, Mihaelos, N. , EPPERSON, C., William , GABRIEL, Sarwat , CASSONE, Domenico , ZIMERI, Jeanny, E.
IPC分类号： A21D2/00
CPC分类号： A21D13/02
摘要： A stabilized bran component and a stabilized whole grain flour containing the component are produced by subjecting a bran-enriched coarse fraction which contains bran, germ and starch to grinding or milling to reduce grittiness of the coarse fraction without substantially damaging the starch due to abrasion. The coarse fraction may be stabilized by heating to substantially reduce the lipase and lipoxygenase activity of the coarse fraction without substantial gelatinization of the starch. High levels of natural antioxidants and vitamins are retained while avoiding substantial acrylamide formation during the stabilization. The stabilized coarse fraction or stabilized bran component may be combined with a fine fraction which contains predominantly endosperm to obtain a stabilized whole grain flour. The stabilized whole grain flours and the stabilized bran component exhibit extended shelf life and may be used for making baked goods, such as cookies, with desirable oven spread, and a non-gritty mouthfeel.
摘要翻译：含有该组分的稳定化的麸皮成分和稳定的全谷物粉是通过对含有麸，胚芽和淀粉的麸富集的粗级分进行研磨或研磨，以减少粗级分的粗糙度而不会由于磨损而基本上损害淀粉来制备的。可以通过加热来稳定粗级分，以显着降低粗级分的脂肪酶和脂氧合酶活性，而不使淀粉明显凝胶化。保留高水平的天然抗氧化剂和维生素，同时在稳定期间避免大量的丙烯酰胺形成。稳定的粗级分或稳定化的麸皮组分可以与主要含有胚乳的精细级分组合以获得稳定的全谷物粉。稳定的全谷物面粉和稳定化的麸皮组分具有延长的保存期限，并且可用于制造烘烤食品，例如饼干，具有理想的烤箱扩散和非粗糙的口感。

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