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11. 发明授权

US5935829A Mutation assays involving blood cells that metabolize toxic substances 失效
标题翻译：涉及代谢有毒物质的血细胞的突变测定
公开(公告)号：US5935829A
公开(公告)日：1999-08-10
申请号：US817971
申请日：1992-01-06
申请人： Charles L. Crespi , William G. Thilly
发明人： Charles L. Crespi , William G. Thilly
IPC分类号： G01N33/50 , C12N15/00 , C12N5/08
CPC分类号： G01N33/5014
摘要： The present invention pertains to a line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity). Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. The invention also includes mutation assays using these cells, and other cells with similar characteristics.
摘要翻译：本发明涉及具有高水平氧化活性（如加氧酶，氧化酶，过氧化物酶和羟化酶活性）的人血细胞系。这种细胞在悬浮培养物中生长，并且可用于确定被代谢为有毒或致突变物质的异种生物物质的致突变性。本发明还包括使用这些细胞的突变测定和具有相似特征的其它细胞。

12. 发明授权

US5128255A Method and a preparation to select for transfected DNA in mammalian cells 失效
标题翻译：在哺乳动物细胞中选择转染DNA的方法和制备方法
公开(公告)号：US5128255A
公开(公告)日：1992-07-07
申请号：US280742
申请日：1988-12-06
申请人： Charles L. Crespi
发明人： Charles L. Crespi
IPC分类号： C12N9/04 , C12N15/64
CPC分类号： C12N9/0006 , C12N15/64
摘要： The invention provides methods and products equal in a selection system for determining the presence or absence of a vector in a mammlian cell. The vector of the invention carries and is capable of expressing a gene encoding a protein capable of effecting the conversion of a precursor to an essential amino acid. A mammalian cell line is created with conditions capable of causing the vector to be introduced into the mammalian cell line. Then, the created mammalian cell line is grown in medium containing a precursors to the essential amino acid but not containing the growth-supporting amount of the essential amino acid and those cells capable of growing are selected.
摘要翻译：本发明提供了在用于确定哺乳动物细胞中载体的存在或不存在的选择系统中相等的方法和产物。本发明的载体携带并能够表达编码能够将前体转化为必需氨基酸的蛋白质的基因。产生哺乳动物细胞系，其条件是能够将载体导入哺乳动物细胞系。然后，将产生的哺乳动物细胞系生长在含有必需氨基酸的前体但不含有必需氨基酸的生长支持量的培养基中，并且选择能够生长的细胞。

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