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    • 16. 发明申请
    • Modified cyanovirin protein
    • 改性氰西林蛋白
    • US20060194226A1
    • 2006-08-31
    • US11331965
    • 2006-01-12
    • Xiaowen LiuKirsten EssenmacherDavid SimpsonQiang Xu
    • Xiaowen LiuKirsten EssenmacherDavid SimpsonQiang Xu
    • C12Q1/68G01N33/53
    • C07K14/195A61K9/0034A61K38/164C07K2319/02
    • This disclosure relates to a modified version of cyanovirin-N protein and a transformed Lactobacillus bacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use of the modified protein as well as the transformed Lactobacillus bacterium. In addition, this disclosure describes two novel promoters originated from L. jensenii, which are capable of directing a high level of gene expression in Lactobacillus bacteria. Also described are an expression cassette comprising one of the novel promoters, a genetically modified Lactobacillus bacterium containing the expression cassette, a method for recombinant gene expression in Lactobacillus bacteria using the novel promoters, and a method for delivery of proteins with desired biological activity to a mucosal surface in a human.
    • 本公开涉及能够重组表达该修饰蛋白质的氰尿素-N蛋白和转化的乳杆菌属细菌的修饰版本。 进一步公开的是编码改性氰尿素-N蛋白的多核苷酸序列,允许修饰蛋白表达的表达盒,以及修饰蛋白以及转化的乳杆菌属细菌的制备和使用方法。 此外,本公开描述了两种起源于延髓酵母的新型启动子,其能够在乳杆菌属细菌中指导高水平的基因表达。 还描述了一种表达盒,其包含新型启动子之一,含有表达盒的经遗传修饰的乳杆菌属细菌,使用该新型启动子在乳杆菌属细菌中重组基因表达的方法,以及将具有所需生物学活性的蛋白质递送至 人体粘膜表面。