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    • 13. 发明申请
    • METHOD FOR PRODUCING AMINOACIDS
    • 用于生产环状氨基转基因生物
    • WO2004057003A3
    • 2004-10-14
    • PCT/EP0314649
    • 2003-12-19
    • METANOMICS GMBH & CO KGAASCHMITZ OLIVERPUZIO PIOTRBLAU ASTRIDLOOSER RALFWENDEL BIRGITKAMLAGE BEATEPLESCH GUNNAR
    • SCHMITZ OLIVERPUZIO PIOTRBLAU ASTRIDLOOSER RALFWENDEL BIRGITKAMLAGE BEATEPLESCH GUNNAR
    • C12N9/10C12N9/88C12N15/82C12P13/12C12N9/02C12N9/92
    • C12P13/12C12N9/1014C12N9/88C12N15/8251C12N15/8253
    • The invention relates to a method for producing aminoacids in transgenic organisms. The inventive method consists of the following steps: a) introduction of nucleic acids sequence which codes threonine decomposing protein or lysine decomposing protein or codes threonine decomposing protein and lysine decomposing protein, b) introduction of nucleic acids sequence which improves the decomposition of threonine or lysine or the decomposition of threonine and lysine in the transgenic organisms; c) expression of (a) or (b) nucleic acids sequence in a transgenic organism. In a very useful manner, the nucleic acids sequence is introduced in the step a) of the method, said sequence being selected from: i) the nucleic acids sequence with the sequence present in SEQ ID NO: 1, SEQ ID NO:11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23 and/or SEQ ID NO:25; ii) the nucleic acids sequence which is preserved as a result of a degenerate genetic code by re-recording aminoacids sequence present in SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24 and/or 26; and iii) a derivative of the nucleic acid sequence present in SEQ ID NO: 1, SEQ ID NO:11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23 and/or SEQ ID NO:25 which codes polypeptides with the nucleic acids sequence present in SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24 and/or 26 and which comprises at least 50 % of homology in terms of aminoacids without reducing the biological activity of polypeptides.
    • 一种用于生产的在转基因生物的氨基酸的方法,其特征在于该方法包括以下步骤:a)将其编码苏氨酸降解蛋白质或赖氨酸分解蛋白质或编码苏氨酸降解蛋白质和赖氨酸分解蛋白质的核酸序列 或b)将增加在转基因生物中的苏氨酸或赖氨酸降解或苏氨酸和赖氨酸降解的核酸序列,和c)表达的下指定的(a)或(b)在所述转基因Organismus.Vorteilhaft的核酸序列是在工艺步骤(a)中 从组i选择的核酸序列)与SEQ ID NO的核酸序列:1,SEQ ID NO:11,SEQ ID NO:13,SEQ ID NO:15,SEQ ID NO:17,SEQ ID NO:19,SEQ ID NO:21,SEQ ID NO:所示;: 23和/或SEQ ID NO 25的序列 ⅱ)的核酸序列由于通过在SEQ ID NO回译遗传密码的简并性:2,SEQ ID NO:12,SEQ ID NO:14,SEQ ID NO:16,SEQ ID NO:18,SEQ ID NO:20 ,SEQ ID NO:22,SEQ ID NO:24和/或SEQ ID NO:得到的氨基酸序列示出26和iii)SEQ ID NO的衍生物:1,SEQ ID NO:11,SEQ ID NO:13,SEQ ID NO:15,SEQ ID NO:17,SEQ ID NO:19,SEQ ID NO:21,SEQ ID NO:23和/或SEQ ID NO:核酸序列代表25,与在SEQ ID NO,其编码的多肽:2 ,SEQ ID NO:12,SEQ ID NO:14,SEQ ID NO:16,SEQ ID NO:18,SEQ ID NO:20,SEQ ID NO:22,SEQ ID NO:24和/或SEQ ID NO:26 编码的氨基酸序列示出,并且具有在没有多肽基本上降低的生物活性的氨基酸水平至少50%的同源性; 介绍。