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11. 发明授权

US07981637B2 Fluorescent protein and chromoprotein 失效
标题翻译：荧光蛋白和荧光蛋白
公开(公告)号：US07981637B2
公开(公告)日：2011-07-19
申请号：US12976579
申请日：2010-12-22
申请人： Atsushi Miyawaki , Satoshi Karasawa
发明人： Atsushi Miyawaki , Satoshi Karasawa
IPC分类号： C12P21/06 , C12N15/00 , C12N1/20 , C07H21/04
CPC分类号： G01N33/542 , C07K14/37 , C07K2319/00 , G01N33/533 , G01N33/582 , G01N2021/6439 , G01N2500/10
摘要： It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from Anthopleura inornata, which have certain property, and fluorescent proteins from Trachyphyllia geoffroyi and Scolymia vitiensis, which have certain fluorescent property.
摘要翻译：本发明的目的是提供一种新型的生色蛋白和新的荧光蛋白。本发明提供了具有一定性质的Anthopleura inornata衍生的色蛋白，以及具有一定荧光性质的来自Trachyphyllia geoffroyi和Scolymia vitiensis的荧光蛋白。

12. 发明授权

US07956172B2 Fluorescent protein and chromoprotein 失效
标题翻译：荧光蛋白和荧光蛋白
公开(公告)号：US07956172B2
公开(公告)日：2011-06-07
申请号：US10561041
申请日：2004-06-16
申请人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
发明人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
IPC分类号： C12N15/12
CPC分类号： C07K14/43504
摘要： It is an object of the present invention to provide a novel fluorescent protein and a novel chromoprotein. The present invention provides a novel fluorescent protein derived from Montipora sp., Acropora sp. and Lobophytum crassum, and a novel chromoprotein derived from Actinia equine.
摘要翻译：本发明的目的是提供一种新的荧光蛋白和新的色蛋白。本发明提供了一种新型的从Montipora sp。，Acropora sp。和Lobophytum crassum，以及衍生自猕猴桃的新型染色体蛋白。

13. 发明申请

US20060240472A1 Pigment protein 失效
标题翻译：颜料蛋白
公开(公告)号：US20060240472A1
公开(公告)日：2006-10-26
申请号：US10516317
申请日：2003-06-10
申请人： Atsushi Miyawaki , Satoshi Karasawa
发明人： Atsushi Miyawaki , Satoshi Karasawa
IPC分类号： G01N33/53 , C07H21/04 , C12P21/06 , C07K14/435
CPC分类号： G01N33/542 , C08L23/02
摘要： An object of the present invention is to provide a novel chromoprotein derived from Cnidopus japonicus. The present invention provides a chromoprotein derived from Cnidopus japonicus having the following properties: (1) the absorption maximum wavelength is 610 nm, and fluorescence is not emitted; (2) the molar absorption coefficient is 66,700 at 610 nm; and (3) the pH sensitivity of light-absorbing property is stable at between pH 4 and pH 10.
摘要翻译：本发明的一个目的是提供一种衍生自日本n a属的新型生色蛋白。本发明提供了具有以下特性的源自日本蝉的色素蛋白：（1）吸收最大波长为610nm，荧光不发射; （2）610 nm处的摩尔吸光系数为66,700; 和（3）在pH4和pH10之间，光吸收性质的pH敏感性是稳定的。

14. 发明申请

US20050106661A1 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US20050106661A1
公开(公告)日：2005-05-19
申请号：US10492081
申请日：2002-10-10
申请人： Atsushi Miyawaki , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Satoshi Karasawa , Toshio Araki
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C07H21/04
CPC分类号： C07K14/43504
摘要： An object of the present invention is to provide a novel fluorescent protein derived from organisms other than Aequorea victoria. According to the present invention, there is provided a fluorescent protein derived from Galaxea fascicularis, which has the following properties: (1) the molecular weight is approximately 27,000; (2) a tetramer is formed in an equilibration state; (3) the excitation maximum wavelength is 492 nm, and the fluorescence maximum wavelength is 505 nm; (4) the molar absorption coefficient is 74,100; (5) the quantum yield is 0.625; and (6) the pH sensitivity of the fluorescent property is low in the range between pH 5 and pH 12.
摘要翻译：本发明的目的是提供一种源自维他命A（Aequorea victoria）以外的生物体的新型荧光蛋白。根据本发明，提供了一种衍生自Galaxea fascicularis的荧光蛋白，其具有以下性质：（1）分子量约为27,000; （2）在平衡状态下形成四聚体; （3）激发最大波长为492nm，荧光最大波长为505nm; （4）摩尔吸光系数为74,100; （5）量子产率为0.625; 和（6）pH5和pH12之间的荧光性能的pH敏感性低。

15. 发明授权

US08207322B2 Fluorescent protein and chromoprotein 失效
标题翻译：荧光蛋白和荧光蛋白
公开(公告)号：US08207322B2
公开(公告)日：2012-06-26
申请号：US13095603
申请日：2011-04-27
申请人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
发明人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
IPC分类号： C12N15/12 , C07K14/435
CPC分类号： C07K14/43504
摘要： It is an object of the present invention to provide a novel fluorescent protein and a novel chromoprotein. The present invention provides a novel fluorescent protein derived from Montipora sp., Acropora sp. and Lobophytum crassum, and a novel chromoprotein derived from Actinia equine.
摘要翻译：本发明的目的是提供一种新的荧光蛋白和新的色蛋白。本发明提供了一种新型的从Montipora sp。，Acropora sp。和Lobophytum crassum，以及衍生自猕猴桃的新型染色体蛋白。

16. 发明授权

US07504491B2 Pigment protein from Cnidopus japonicus 失效
标题翻译：来自日本的颜料蛋白
公开(公告)号：US07504491B2
公开(公告)日：2009-03-17
申请号：US10516317
申请日：2003-06-10
申请人： Atsushi Miyawaki , Satoshi Karasawa
发明人： Atsushi Miyawaki , Satoshi Karasawa
IPC分类号： C07H21/04 , C07K1/00
CPC分类号： G01N33/542 , C08L23/02
摘要： An object of the present invention is to provide a chromoprotein derived from Cnidopus japonicus. The present invention provides a chromoprotein derived from Cnidopus japonicus having the following properties: (1) the absorption maximum wavelength is 610 nm, and fluorescence is not emitted; (2) the molar absorption coefficient is 66,700 at 610 nm; and (3) the pH sensitivity of light-absorbing property is stable at between pH 4 and pH 10.
摘要翻译：本发明的目的是提供衍生自日本n a属的生色蛋白。本发明提供了具有以下特性的源自日本蝉的色素蛋白：（1）吸收最大波长为610nm，荧光不发射; （2）610 nm处的摩尔吸光系数为66,700; 和（3）在pH4和pH10之间，光吸收性质的pH敏感性是稳定的。

17. 发明授权

US07960530B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US07960530B2
公开(公告)日：2011-06-14
申请号：US10561040
申请日：2004-06-16
申请人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
发明人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
IPC分类号： C07H21/04 , C12P21/06
CPC分类号： C07K14/43595
摘要： An object of the present invention is to provide a novel fluorescent protein derived from favia favus. The present invention provides a fluorescent protein derived from favia favus having the following properties: (1) an excitation maximum wavelength is 507 nm; (2) a fluorescence maximum wavelength is 517 nm; (3) a molar absorption coefficient at 482 nm is 80,000; (4) a quantum yield is 0.68; and (5) pH sensitivity of the fluorescence maximum is stable at pH 5 to pH 11.
摘要翻译：本发明的一个目的是提供一种来源于粉刺的新型荧光蛋白。本发明提供具有以下特性的源自粉刺的荧光蛋白：（1）激发最大波长为507nm; （2）荧光最大波长为517nm; （3）在482nm处的摩尔吸光系数为80,000; （4）量子产率为0.68; 和（5）荧光最大的pH敏感性在pH5至pH11下是稳定的。

18. 发明申请

US20090170073A1 FLUORESCENT PROTEIN AND CHROMOPROTEIN 失效
标题翻译：荧光蛋白和色氨酸蛋白
公开(公告)号：US20090170073A1
公开(公告)日：2009-07-02
申请号：US10561041
申请日：2004-06-16
申请人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
发明人： Atsushi Miyawaki , Hidekazu Tsutsui , Satoshi Karasawa
IPC分类号： C12Q1/68 , C07K2/00 , C07H21/04
CPC分类号： C07K14/43504
摘要： It is an object of the present invention to provide a novel fluorescent protein and a novel chromoprotein. The present invention provides a novel fluorescent protein derived from Montipora sp., Acropora sp. and Lobophytum crassum, and a novel chromoprotein derived from Actinia equine.
摘要翻译：本发明的目的是提供一种新的荧光蛋白和新的色蛋白。本发明提供了一种新型的从Montipora sp。，Acropora sp。和Lobophytum crassum，以及衍生自猕猴桃的新型染色体蛋白。

19. 发明申请

US20070031912A1 Method for analysis of protein interaction using fluorescent protein 审中-公开
标题翻译：使用荧光蛋白分析蛋白质相互作用的方法
公开(公告)号：US20070031912A1
公开(公告)日：2007-02-08
申请号：US11390215
申请日：2006-03-28
申请人： Atsushi Miyawaki , Satoshi Karasawa
发明人： Atsushi Miyawaki , Satoshi Karasawa
IPC分类号： C12Q1/37
CPC分类号： G01N33/542
摘要： An object of the present invention is to provide a method for analyzing the protein interaction, wherein the information about time can be obtained and the movement of protein can be monitored. The present invention provides a method for analyzing interaction between a first test protein and a second test protein which comprises the steps of: splitting a fluorescent protein capable of emitting different color of fluorescence according to passage of time into an N-terminal fragment and a C-terminal fragment; allowing the first test protein to interact with the second test protein by making coexist a fusion protein of the N-terminal fragment with the first test protein and another fusion protein of the C-terminal fragment with the second test protein; and detecting the change in the fluorescent light due to the interaction.
摘要翻译：本发明的目的是提供一种用于分析蛋白质相互作用的方法，其中可以获得关于时间的信息，并且可以监测蛋白质的移动。本发明提供了一种用于分析第一测试蛋白质和第二测试蛋白质之间的相互作用的方法，其包括以下步骤：将能够根据时间流逝发射不同颜色的荧光的荧光蛋白分成N末端片段和C 末端片段通过使N末端片段的融合蛋白与第一测试蛋白和C末端片段的另一融合蛋白与第二测试蛋白共存，允许第一测试蛋白与第二测试蛋白相互作用; 并且检测由于相互作用引起的荧光灯的变化。

20. 发明授权

US08420781B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US08420781B2
公开(公告)日：2013-04-16
申请号：US13196499
申请日：2011-08-02
申请人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
发明人： Atsushi Miyawaki , Takako Kogure , Hiroshi Hama , Masataka Kinjo , Kenta Saito , Satoshi Karasawa , Toshio Araki
IPC分类号： C07K1/00 , C08B37/08
CPC分类号： C07K14/37 , C07K14/43504 , G01N33/533
摘要： An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.
摘要翻译：本发明的目的是提供一种红色或橙色荧光蛋白，其特征在于，激发峰值（最大吸收波长）与荧光峰值（最大荧光的波长）之间的差异（斯托克斯偏移）为从而可以通过最大激发获得最大荧光。本发明提供了一种新颖的荧光蛋白，其通过将突变引入到来自真菌属的荧光蛋白中而单体化，以及衍生自Montipora的新型生色蛋白和荧光蛋白。 sp。

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