专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明申请

US20100292310A1 MICRO-RNA SCAFFOLDS AND NON-NATURALLY OCCURRING MICRO-RNAS 有权
标题翻译： MICRO-RNA SCAFFOLDS和非天然产生的微RNA
公开(公告)号：US20100292310A1
公开(公告)日：2010-11-18
申请号：US12601508
申请日：2008-05-22
申请人： Melissa Kelley , Amanda Birmingham , Jon Karpilow , Anastasia Khvorova , Kevin Sullivan
发明人： Melissa Kelley , Amanda Birmingham , Jon Karpilow , Anastasia Khvorova , Kevin Sullivan
IPC分类号： A61K31/7088 , C07H21/02 , C12N15/63 , C12N5/10
CPC分类号： C12N15/113 , C12N15/111 , C12N2310/141 , C12N2310/531 , C12N2320/30 , C12N2330/30 , C12Q1/6869
摘要： The present disclosure provides a non-naturally occurring miRNA having a stem-loop structure comprising a scaffold derived from a first endogenous miRNA (e.g., miR-196a-2 or miR-204), a mature strand derived from a second endogenous miRNA, and a star strand sequence that is at least partially complementary to the mature strand sequence. The present disclosure also provides a non-naturally occurring miRNA having a stem-loop structure comprising a scaffold derived from an endogenous miRNA (e.g., miR-196a-2 or miR-204), a mature strand designed t be at least partially complementary to a target RNA, and a star strand sequence that is at least partially complementary to the mature strand sequence. The methods and compositions of the disclosure may be used to mediate gene silencing via the RNAi pathway.
摘要翻译：本公开提供了非天然存在的具有茎环结构的miRNA，其包含源自第一内源miRNA（例如，miR-196a-2或miR-204）的支架，源自第二内源miRNA的成熟链，以及与成熟链序列至少部分互补的星形链序列。本公开还提供了非天然存在的miRNA，其具有包含源自内源miRNA（例如，miR-196a-2或miR-204）的支架的茎环结构，成熟链被设计为至少部分地与靶RNA和与成熟链序列至少部分互补的星形链序列。本公开的方法和组合物可用于通过RNAi途径介导基因沉默。

12. 发明授权

US08841267B2 Micro-RNA scaffolds, non-naturally occurring micro-RNAs, and methods for optimizing non-naturally occurring micro-RNAs 有权
标题翻译：微RNA支架，非天然存在的微RNA，以及优化非天然存在的微RNA的方法
公开(公告)号：US08841267B2
公开(公告)日：2014-09-23
申请号：US12601506
申请日：2008-05-22
申请人： Melissa Kelley , Amanda Birmingham , Jon Karpilow , Anastasia Khvorova , Kevin Sullivan
发明人： Melissa Kelley , Amanda Birmingham , Jon Karpilow , Anastasia Khvorova , Kevin Sullivan
IPC分类号： C12N15/11 , C12N15/64 , C07H21/04
CPC分类号： C12N15/113 , C12N15/111 , C12N2310/141 , C12N2310/531 , C12N2320/30 , C12N2330/30 , C12Q1/6869
摘要： The present disclosure provides non-naturally occurring miR-196a-2 miRNAs and non-naturally occurring miR-204 miRNAs. The non-naturally occurring miRNAs of the disclosure have mature strand sequences distinct from their endogenous counterparts. The disclosure also provides methods of selecting mature strand sequences that function optimally in non-naturally occurring miR-196a-2 miRNAs. The methods and compositions of the disclosure may be used to mediate gene silencing via the RNAi pathway.
摘要翻译：本公开提供非天然存在的miR-196a-2 miRNA和非天然存在的miR-204 miRNA。本公开的非天然存在的miRNA具有与其内源对应物不同的成熟链序列。本公开还提供了选择在非天然存在的miR-196a-2 miRNA中最佳起作用的成熟链序列的方法。本公开的方法和组合物可用于通过RNAi途径介导基因沉默。

13. 发明申请

US20050203043A1 Identification of toxic nucleotide sequences 审中-公开
标题翻译：鉴定毒性核苷酸序列
公开(公告)号：US20050203043A1
公开(公告)日：2005-09-15
申请号：US11040553
申请日：2005-01-21
申请人： Yuriy Fedorov , Jon Karpilow , Anastasia Khvorova
发明人： Yuriy Fedorov , Jon Karpilow , Anastasia Khvorova
IPC分类号： C07H21/02 , C07H21/04 , C12N15/11 , C12Q1/68 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12Q1/6883 , C12N15/111 , C12N2310/14 , C12N2310/53 , C12N2320/10 , C12Q2600/142
摘要： Toxic nucleic acid sequences and methods for identifying, using, and screening libraries for them, including in silico screening, are provided. Compositions of the invention comprise unimolecular and double stranded polynucleotides comprising at least one toxicity region. Toxic sequences of the invention include A/G UUU A/G/U, G/C AAA G/C, and/or GCCA, NUUU, wherein N is any nucleotide, or complements thereof. The invention also provides a method of inducing a toxic response in a cell, comprising introducing into the cell a unimolecular or double stranded polynucleotide comprising at least one toxicity region comprising a sequence selected from the group consisting of A/G UUU A/G/U, G/C AAA G/C, GAAT, and GCCA, NUUU, wherein N is any nucleotide, or a complement of any of the foregoing, wherein said unimolecular or double stranded polynucleotide is at least 5 base pairs long and is comprises a sense and antisense region that are at least substantially complementary.
摘要翻译：提供了有毒的核酸序列和用于识别，使用和筛选其文库的方法，包括计算机筛选。本发明的组合物包含包含至少一个毒性区域的单分子和双链多核苷酸。本发明的毒性序列包括A / G UUU A / G / U，G / C AAA G / C和/或GCCA，NUUU，其中N是任何核苷酸或其互补序列。本发明还提供了在细胞中诱导毒性反应的方法，包括向细胞中引入单分子或双链多核苷酸，所述单分子或双链多核苷酸包含至少一个毒性区，其包含选自A / G UUU A / G / U ，G / C AAA G / C，GAAT和GCCA，NUUU，其中N是任何前述的任何核苷酸或补体，其中所述单分子或双链多核苷酸长度为至少5个碱基对并且包含感觉和至少基本上互补的反义区。

14. 发明申请

US20140066595A1 Modulators of Protein Production in a Human Cell Line and Cell-free Extracts Produced Therefrom 审中-公开
标题翻译：人类细胞系中的蛋白质生产调节剂及其生产的无细胞提取物
公开(公告)号：US20140066595A1
公开(公告)日：2014-03-06
申请号：US14010246
申请日：2013-08-26
申请人： Emily Anderson , Peter Bell , Penny Jensen , Jon Karpilow , Anja Smith , Krishna Vattem , Brian Webb , Devin Leake , Alex Medford , Elena Maksimova , Craig Smith
发明人： Emily Anderson , Peter Bell , Penny Jensen , Jon Karpilow , Anja Smith , Krishna Vattem , Brian Webb , Devin Leake , Alex Medford , Elena Maksimova , Craig Smith
IPC分类号： C12P21/00 , C07K14/00
CPC分类号： C12P21/00 , C07K14/00
摘要： Cell-free extracts, methods for producing these extracts, methods for using these extracts, compositions that facilitate production of these extracts and kits that contain these extracts are provided. By increasing or decreasing certain gene products through, for example, the use of siRNA or mimics, one can develop mammalian cell-free extracts that have desired levels of efficiency.
摘要翻译：提供了无细胞提取物，用于生产这些提取物的方法，使用这些提取物的方法，促进这些提取物生产的组合物和含有这些提取物的试剂盒。通过例如使用siRNA或模拟物来增加或减少某些基因产物，可以开发具有期望的效率水平的哺乳动物无细胞提取物。

15. 发明申请

US20120083035A1 Modified Cell Lines for Increasing Lentiviral Titers 审中-公开
标题翻译：改良细胞系增加慢病毒滴度
公开(公告)号：US20120083035A1
公开(公告)日：2012-04-05
申请号：US13222509
申请日：2011-08-31
申请人： Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
发明人： Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
IPC分类号： C12N5/073 , C12N5/02
CPC分类号： C12N15/113 , C12N15/86 , C12N2740/15051 , C12N2740/15052
摘要： The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.
摘要翻译：本公开提供了一种修饰细胞以增强慢病毒滴度，经修饰和修饰试剂的细胞系的方法。通过介导个体基因及其组合，可以增加慢病毒滴度。

16. 发明授权

US09284552B2 Modified cell lines for increasing lentiviral titers 有权
标题翻译：用于增加慢病毒滴度的改良细胞系
公开(公告)号：US09284552B2
公开(公告)日：2016-03-15
申请号：US13854995
申请日：2013-04-02
申请人： Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
发明人： Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
IPC分类号： C12N7/01 , C12N7/02 , C12N15/86 , A61K39/21 , C12N15/113
CPC分类号： C12N15/113 , C12N15/86 , C12N2740/15051 , C12N2740/15052
摘要： The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.
摘要翻译：本公开提供了一种修饰细胞以增强慢病毒滴度，经修饰和修饰试剂的细胞系的方法。通过介导个体基因及其组合，可以增加慢病毒滴度。

17. 发明申请

US20130217121A1 Modified Cell Lines for Increasing Lentiviral Titers 有权
标题翻译：改良细胞系增加慢病毒滴度
公开(公告)号：US20130217121A1
公开(公告)日：2013-08-22
申请号：US13854995
申请日：2013-04-02
申请人： Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
发明人： Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , C12N15/86 , C12N2740/15051 , C12N2740/15052
摘要： The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.
摘要翻译：本公开提供了一种修饰细胞以增强慢病毒滴度，经修饰和修饰试剂的细胞系的方法。通过介导个体基因及其组合，可以增加慢病毒滴度。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式