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    • 11. 发明申请
    • Microinjection equipment
    • 显微注射设备
    • US20080102516A1
    • 2008-05-01
    • US11600810
    • 2006-11-17
    • Jun SasakiAkihiko YabukiShusaku NishiyamaIkushi Takasaki
    • Jun SasakiAkihiko YabukiShusaku NishiyamaIkushi Takasaki
    • C12M1/36C12M3/00
    • C12M35/00
    • To provide a microinjection equipment capable of fitting a micro needle to a manipulator etc. manipulating the micro needle in short time on the occasion of an injection operation. Included are a micro needle transporting device 12 detachably grasping a micro needle 10 filled with an introduction substance and disposed in a predetermined standby position and transporting the micro needle 10 to a predetermined ready-for-introducing position, a manipulator 14 grasping in the attachable/detachable manner the micro needle 10 disposed in the predetermined ready-for-introducing position and inserting and removing the tip of the micro needle 10 into and from an introduction target micro body 13, and an introduction substance discharging device 15 discharging the introduction substance in the micro needle 10 by applying a predetermined pressure to an interior of the micro needle 10 grasped by the manipulator 14.
    • 提供能够将微针装配到操纵器等的显微注射设备,以在注射操作时在短时间内操纵微针。 包括微针运送装置12,其可拆卸地抓住填充有引入物质并且设置在预定待机位置的微针10并将微针10运送到预定的准备导入位置,操纵器14抓握在可附接/ 将微针10配置在规定的准备导入位置并将微针10的前端插入导入对象微体13内,从导入对象微体13排出的引导物质排出装置15, 微针10通过对由操纵器14掌握的微针10的内部施加预定的压力。
    • 14. 发明授权
    • Microinjection device and microinjection method
    • 显微注射装置和显微注射法
    • US08859224B2
    • 2014-10-14
    • US11785410
    • 2007-04-17
    • Kiyoshi TaninakaJun SasakiAkihiko Yabuki
    • Kiyoshi TaninakaJun SasakiAkihiko Yabuki
    • C12Q1/02C12M1/42C12M1/34
    • C12M35/00C12M41/00C12M41/40C12M41/44
    • A fluorescence-intensity detecting unit detects fluorescence intensity by injecting a first solution containing a fluorescent reagent into a second solution that does not form an interface with the first solution through an injecting member. A calculating unit calculates the injection amount of the first solution from the fluorescence intensity based on a correlation between fluorescence intensities and injection amounts measured in advance. A computing unit obtains a correlation between an injection amount, pressure and pressurizing time based on the calculated injection amount. An adjusting unit adjusts the amount of the first solution to be injected into the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation.
    • 荧光强度检测单元通过将含有荧光试剂的第一溶液通过注射部件注入到与第一溶液不形成界面的第二溶液中来检测荧光强度。 计算单元基于荧光强度和预先测量的注射量之间的相关性,从荧光强度计算第一溶液的注射量。 计算单元基于所计算的喷射量来获得喷射量,压力和加压时间之间的相关性。 调整单元通过基于获得的相关性来控制压力和加压时间来调节要注入内质网的第一溶液的量。
    • 17. 发明授权
    • Microinjection equipment
    • 显微注射设备
    • US07855067B2
    • 2010-12-21
    • US11600810
    • 2006-11-17
    • Jun SasakiAkihiko YabukiShusaku NishiyamaIkushi Takasaki
    • Jun SasakiAkihiko YabukiShusaku NishiyamaIkushi Takasaki
    • C12M3/00C12M1/26C12M1/36
    • C12M35/00
    • To provide a microinjection equipment capable of fitting a micro needle to a manipulator etc. manipulating the micro needle in short time on the occasion of an injection operation. Included are a micro needle transporting device 12 detachably grasping a micro needle 10 filled with an introduction substance and disposed in a predetermined standby position and transporting the micro needle 10 to a predetermined ready-for-introducing position, a manipulator 14 grasping in the attachable/detachable manner the micro needle 10 disposed in the predetermined ready-for-introducing position and inserting and removing the tip of the micro needle 10 into and from an introduction target micro body 13, and an introduction substance discharging device 15 discharging the introduction substance in the micro needle 10 by applying a predetermined pressure to an interior of the micro needle 10 grasped by the manipulator 14.
    • 提供能够将微针装配到操纵器等的显微注射设备,以在注射操作时在短时间内操纵微针。 包括微针运送装置12,其可拆卸地抓住填充有引入物质并且设置在预定待机位置的微针10并将微针10运送到预定的准备导入位置,操纵器14抓握在可附接/ 将微针10配置在规定的准备导入位置并将微针10的前端插入导入对象微体13内,从导入对象微体13排出的引导物质排出装置15, 微针10通过对由操纵器14掌握的微针10的内部施加预定的压力。
    • 18. 发明申请
    • Microinjection device and microinjection method
    • 显微注射装置和显微注射法
    • US20080206802A1
    • 2008-08-28
    • US11785410
    • 2007-04-17
    • Kiyoshi TaninakaJun SasakiAkihiko Yabuki
    • Kiyoshi TaninakaJun SasakiAkihiko Yabuki
    • C12Q1/02C12M1/36
    • C12M35/00C12M41/00C12M41/40C12M41/44
    • A fluorescence-intensity detecting unit detects fluorescence intensity by injecting a first solution containing a fluorescent reagent into a second solution that does not form an interface with the first solution through an injecting member. A calculating unit calculates the injection amount of the first solution from the fluorescence intensity based on a correlation between fluorescence intensities and injection amounts measured in advance. A computing unit obtains a correlation between an injection amount, pressure and pressurizing time based on the calculated injection amount. An adjusting unit adjusts the amount of the first solution to be injected into the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation.
    • 荧光强度检测单元通过将含有荧光试剂的第一溶液通过注射部件注入到与第一溶液不形成界面的第二溶液中来检测荧光强度。 计算单元基于荧光强度和预先测量的注射量之间的相关性,从荧光强度计算第一溶液的注射量。 计算单元基于所计算的喷射量来获得喷射量,压力和加压时间之间的相关性。 调整单元通过基于获得的相关性来控制压力和加压时间来调节要注入内质网的第一溶液的量。
    • 20. 发明申请
    • Assembling method and device for magneto-optical head
    • 磁光头组装方法及装置
    • US20050063254A1
    • 2005-03-24
    • US10986676
    • 2004-11-12
    • Akihiko YabukiMamoru HokariShigeru AkemaAyu Miyakawa
    • Akihiko YabukiMamoru HokariShigeru AkemaAyu Miyakawa
    • G11B7/22G11B11/105G11B7/00
    • G11B11/10532G11B7/22G11B11/10554
    • An assembling device for a magneto-optical head including a glass plate having a first surface and a second surface parallel to each other with a coil formed on the first surface, and a semispherical lens bonded to the second surface of the glass plate. The assembling device includes an XY stage movable in an X-axis direction and a Y-axis direction orthogonal to each other, a jig mounted on the XY stage, a grip mechanism for gripping the semispherical lens, and an XYZ stage for carrying the grip mechanism so that the grip mechanism is movable in the X-axis direction, the Y-axis direction, and a Z-axis direction orthogonal to each other. The assembling device further includes an interference microscope for observing the glass plate placed on the jig and the semispherical lens, a Z stage for supporting the interference microscope so that the interference microscope is movable in the Z-axis direction, an image pickup unit for picking up an observed image from the interference microscope, an image processing unit connected to the image pickup unit, and a control unit connected to the XY stage, the XYZ stage, the Z stage, and the image processing unit.
    • 一种用于磁光头的组装装置,包括具有形成在第一表面上的线圈彼此平行的第一表面和第二表面的玻璃板,以及接合到玻璃板的第二表面的半球形透镜。 组装装置包括:XY台,其能够在X轴方向和Y轴方向上相互正交地移动;安装在XY台上的夹具;夹持半球形透镜的夹持机构;以及用于承载手柄的XYZ平台 机构,使得夹持机构能够在彼此正交的X轴方向,Y轴方向和Z轴方向上移动。 组装装置还包括用于观察放置在夹具上的玻璃板和半球形透镜的干涉显微镜,用于支撑干涉显微镜以使干涉显微镜能够沿Z轴方向移动的Z平台,用于拾取的图像拾取单元 从干涉显微镜观察观察到的图像,连接到图像拾取单元的图像处理单元,以及连接到XY平台,XYZ平台,Z平台和图像处理单元的控制单元。