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    • 12. 发明公开
    • 마이크로플루이딕 장치, 장치의 제조방법 및 하이드로젤에 세포를 담지하는 방법
    • 微流控系统及其制造方法及其在水凝胶中的细胞渗透方法
    • KR1020150133402A
    • 2015-11-30
    • KR1020140060033
    • 2014-05-19
    • 한국과학기술원
    • 이석재이문근이태재이경균김도현박견주석승환
    • C12M3/08
    • C12M3/08C12M1/00
    • 본발명은세포가하이드로젤에담지되는기술에관한것으로, 더욱상세하게는미세기둥어레이존과포커싱존을통과하면서단일세포로분산되어하이드로젤에담지되는마이크로플루이딕장치, 장치의제조방법및 하이드로젤에세포를담지하는방법에관한것이다. 본발명인마이크로플루이딕장치를이용한세포담지방법을적용하면, 미세기둥어레이존이라는구조체를이용하여뭉쳐있는구형이아닌미생물세포를분산시키고, 포커싱존에서하이드로젤방울에단일세포의형태로담지함으로써, 고분자로인한세포의뭉침문제를해결하고분리된세포를단일세포의형태로하이드로젤에담지하는것이가능하다.
    • 本发明涉及一种在水凝胶中封装细胞的技术,更具体地,涉及一种微流体装置,用于允许细胞在通过细列阵列区和聚焦区时分散到单个细胞中,并被支撑在 水凝胶,以及微流体装置的制造方法以及在水凝胶中负载细胞的方法。 当使用使用本发明的微流体装置的细胞支持方法时,可以通过使用细列阵列区的结构来分散不具有聚集球形的微生物细胞。 此外,微生物细胞可以在聚焦区域中以单细胞的形式支持在水凝胶滴中,从而解决由聚合物引起的细胞聚集的问题,并将分散的细胞以 单细胞
    • 13. 发明公开
    • 집적형 회전 PCR 방법 및 이를 위한 집적형 회전 PCR용 마이크로칩
    • 一体化旋转PCR方法和微型PCR方法
    • KR1020150031912A
    • 2015-03-25
    • KR1020130111719
    • 2013-09-17
    • 한국과학기술원
    • 서태석정재환김도현
    • G01N35/08C12M1/36C12M1/38
    • The present invention provides a microchip for integrated rotary PCR including a unit processing unit that is positioned apart from a rotation center in a radial direction, in which the unit processing unit is positioned on a further downstream side than a part provided with a target material capturing unit that is provided with a capturing passage having an inlet and an outlet and capturing means with which the capturing passage is filled; a sample storage unit that is positioned radially inward compared to the target material capturing unit, is connected to the inlet of the capturing passage, and provides an inner space for sample storage; a cleaning buffer solution storage unit that is positioned radially inward compared to the sample storage unit, is connected to the inlet of the capturing passage, and provides an inner space for cleaning buffer solution storage; a discharge passage that is connected to the outlet of the capturing passage and extends to be positioned radially outward away from the outlet; a first elution buffer solution storage unit that is connected to the discharge passage and provides an inner space for first elution buffer solution storage; a second elution buffer solution storage unit that is positioned radially inward compared to the first elution buffer solution storage unit, is connected to the inlet of the capturing passage, and provides an inner space for second elution buffer solution storage; a waste solution chamber that is connected to the discharge passage and is positioned on a radially outward side of the discharge passage; and a target material chamber that is connected to the discharge passage and is positioned on a radially outward side of the discharge passage.
    • 本发明提供了一种用于集成旋转PCR的微芯片,其包括单元处理单元,该单元处理单元沿径向方向与旋转中心分离,单元处理单元位于比设置有目标材料捕获的部分更远的下游侧 设置有具有入口和出口的捕获通道的单元,并且捕获通道用于捕获装置; 与目标材料捕获单元相比位于径向向内的样品存储单元连接到捕获通道的入口,并提供用于样品存储的内部空间; 与样品存储单元相比位于径向向内的清洁缓冲溶液存储单元连接到捕获通道的入口,并提供用于清洁缓冲溶液储存的内部空间; 排出通道,其连接到捕获通道的出口并延伸以远离出口径向向外定位; 第一洗脱缓冲液存储单元,其连接到排出通道并提供用于第一洗脱缓冲溶液储存的内部空间; 与第一洗脱缓冲液存储单元相比,径向向内定位的第二洗脱缓冲液存储单元连接到捕获通道的入口,并提供用于第二洗脱缓冲液存储的内部空间; 废液室,其连接到排出通道并位于排出通道的径向向外侧; 以及连接到排出通道并位于排出通道的径向外侧的目标材料室。
    • 17. 发明公开
    • 형광 중공 실리카 나노입자 및 이의 제조방법
    • 荧光二氧化硅纳米微粒及其制备方法
    • KR1020120005749A
    • 2012-01-17
    • KR1020100066381
    • 2010-07-09
    • 한국과학기술원
    • 김도현이경균위린복이석재박태정
    • C09K11/02A61K9/51
    • PURPOSE: Fluorescent hollow silica nanoparticles and a producing method thereof are provided to stably mass produce a core unit with a big hollow hole inside the nanoparticles. CONSTITUTION: Fluorescent hollow silica nanoparticles comprise the following: a core unit with a hollow hole having the diameter of 1-100nm; a silica shell unit surrounding the core unit; and fluorescent materials contained in the silica shell unit. A producing method of the fluorescent hollow silica nanoparticles comprises the following steps: applying the fluorescent materials to a synthesis process of silica nanoparticle; and processing the obtained fluorescent silica nanoparticles with hydrofluoric acid for producing pores inside the nanoparticles.
    • 目的:提供荧光中空二氧化硅纳米颗粒及其制备方法,以稳定地大量生产纳米颗粒内部具有大空心孔的芯单元。 构成:荧光中空二氧化硅纳米颗粒包括以下:具有中空孔的核心单元,其直径为1-100nm; 围绕所述核心单元的二氧化硅壳单元; 和包含在二氧化硅壳单元中的荧光材料。 荧光中空二氧化硅纳米颗粒的制备方法包括以下步骤:将荧光物质施加到二氧化硅纳米颗粒的合成过程中; 并用氢氟酸处理得到的荧光二氧化硅纳米粒子,以在纳米粒子内产生孔。
    • 20. 发明公开
    • 데이터 중심 저장 센서 네트워크에서의 부하 감쇄 방법 및 센서 노드
    • 在数据中心存储传感器网络和传感器节点中减少OVERHEAD的方法
    • KR1020110088738A
    • 2011-08-04
    • KR1020100008380
    • 2010-01-29
    • 한국과학기술원
    • 이영희황수안퉁김도현
    • H04L12/28H04W84/18H04W40/20
    • H04W40/20H04W40/18H04W84/18
    • PURPOSE: A method for reducing overhead in a data centric storage sensor network and a sensor node are provided to reduce a perimeter work by establishing most adjacent node as a rendezvous node of an event. CONSTITUTION: If a get or put request of an application unit(501) or a sensor unit is generated, an indexing unit(505) performs hashing of a key value. A location based routing unit(507) generates a packet for transmitting to a rendezvous node from a GPSR(Greedy Perimeter Stateless Routing) or a GOAFR+(Greedy Routing And Face Routing+). The location based routing unit transmits the packet to an MAC protocol unit(509). A parameter work control unit(508) prevents a perimeter work about a packet which is arrived to an adjacent node around the destination of the packet.
    • 目的:提供一种减少数据中心存储传感器网络和传感器节点开销的方法,通过建立大多数相邻节点作为事件的会合点来减少周边工作。 构成:如果产生应用单元(501)或传感器单元的获取或放置请求,索引单元(505)执行键值的散列。 基于位置的路由单元(507)生成用于从GPSR(贪婪周边无状态路由)或GOAFR +(贪婪路由和面部路由+)发送到会合节点的分组。 基于位置的路由单元将分组发送到MAC协议单元(509)。 参数工作控制单元(508)防止关于到达分组目的地周围的相邻节点的分组的周边工作。