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11. 发明申请

US20080231834A1 SURFACE PLASMON ASSISTED MICROSCOPE 有权
标题翻译：表面等离子体辅助显微镜
公开(公告)号：US20080231834A1
公开(公告)日：2008-09-25
申请号：US12018107
申请日：2008-01-22
申请人： Zygmunt Gryczynski , Ignacy Gryczynski , Nils Calander , Julian Borejdo
发明人： Zygmunt Gryczynski , Ignacy Gryczynski , Nils Calander , Julian Borejdo
IPC分类号： G01N1/00
CPC分类号： G01Q60/20 , G01N21/6428 , G01N21/6458 , G01N21/648 , G01N2021/6482 , G02B21/0076
摘要： The present invention includes a microscope and a method for using the microscope for single molecule with reduced photobleaching of a fluorophore (20) that includes a light translucent material (16); a metal layer (18) disposed on the light translucent material (16); a medium (15) disposed on the metal layer (18), the medium (15) having one or more fluorophores (20) capable of binding a target analyte (e.g., inside a cell); a microscope positioned to observe the surface plasmon emissions from the one or more fluorophores (20) within 50 nanometers of the surface of the metal layer (18); an excitation source capable of exciting the one or more fluorophores (20), the excitation source positioned to strike the light translucent material (16) at a first angle; and a light detector (38) that selectively detects emitted light generated by excited fluorophores (20) at a second angle (22), wherein light emitted by the one or more fluorophores (20) at the surface plasmon angle is detected through the microscope, such that single molecules may be detected without significantly degrading fluorophore (20) emissions.
摘要翻译：本发明包括一种显微镜和一种使用该显微镜用于具有减少的光漂白的单分子的显微镜，所述荧光团（20）包括光半透明材料（16）; 设置在所述光半透明材料（16）上的金属层（18）; 设置在所述金属层（18）上的介质（15），所述介质（15）具有一个或多个能够结合目标分析物（例如细胞内）的荧光团（20）; 位于观察来自金属层（18）的表面的50纳米内的一个或多个荧光团（20）的表面等离子体激元发射的显微镜; 激励源，其能够激发所述一个或多个荧光团（20），所述激发源被定位成以第一角度撞击所述光半透明材料（16）; 以及光检测器（38），其以第二角度（22）选择性地检测由激发的荧光团（20）产生的发射光，其中通过显微镜检测由所述一个或多个荧光团（20）以表面等离子体角度发射的光，使得可以在不显着降解荧光团（20）排放的情况下检测单分子。

12. 发明申请

US20050031536A1 Methods and compositions comprising monitoring devices 审中-公开
标题翻译：包括监测装置的方法和组合物
公开(公告)号：US20050031536A1
公开(公告)日：2005-02-10
申请号：US10886388
申请日：2004-07-07
申请人： Zygmunt Gryczynski , Candace McCombs , Joseph Lakowicz , Omoefe Abugo
发明人： Zygmunt Gryczynski , Candace McCombs , Joseph Lakowicz , Omoefe Abugo
IPC分类号： A61K49/00 , G06F19/00
CPC分类号： G06F19/3456 , A61B5/0071 , A61B5/4833 , A61B5/681 , A61K49/0034 , A61K49/0041 , A61K49/0063 , A61K49/0082 , Y02A90/26
摘要： Methods and compositions for the detection and monitoring of drug therapy are provided. In particular, efficient and sensitive methods for the detection of drug ingestion are provided for determining whether individuals are complying with prescribed therapeutic regimens, and for providing a mechanism for identifying drug-resistant strains of infectious agents. The claimed methods and compositions involve the application of transdermal devices containing detection mechanisms for receiving and recording signals generated by the ingestion of a labeled drug. Such devices are attached to the skin for the duration of drug therapy and compliance is determined either by direct reading, or by remote monitoring whereby signals are transmitted from the device and received at an external site such as a healthcare facility.
摘要翻译：提供了用于检测和监测药物治疗的方法和组合物。特别地，提供用于检测药物摄入的有效和敏感的方法用于确定个体是否符合规定的治疗方案，以及提供用于鉴定感染性药物的耐药菌株的机制。所要求保护的方法和组合物涉及应用含有检测机制的透皮装置，用于接收和记录由摄取标记药物产生的信号。这样的装置在药物治疗期间附着到皮肤，并且通过直接读取或通过远程监测来确定顺应性，其中信号从装置传输并在诸如医疗保健设施的外部部位接收。

13. 发明授权

US06660379B1 Luminescence spectral properties of CdS nanoparticles 失效
标题翻译： CdS纳米粒子的发光光谱性质
公开(公告)号：US06660379B1
公开(公告)日：2003-12-09
申请号：US09890042
申请日：2001-07-25
申请人： Joseph R. Lakowicz , Ignacy Gryczynski , Zygmunt Gryczynski
发明人： Joseph R. Lakowicz , Ignacy Gryczynski , Zygmunt Gryczynski
IPC分类号： B32B516
CPC分类号： G01N33/588 , B82Y15/00 , C09K11/025 , C09K11/565 , C12Q1/6816 , Y10T428/2982 , Y10T428/2991 , Y10T428/2998
摘要： The steady state and time resolved luminescence spectral properties of two types of novel CdS nanoparticles and nanoparticles are described. CdS nanoparticles formed in the presence of an amine-terminated dendrimer show blue emission. The emission wavelength of these nanoparticles depended on the excitation wavelength. The CdS/dendrimer nanoparticles display polarized emission with the anisotropy rising progressively from 340 to 420 nm excitation, reaching a maximal anisotropy value in excess of 0.3. A new constant positive polarized emission from luminescent nanoparticles is also described. Polyphosphate-stabilized CdS nanoparticles are described that display a longer wavelength red emission maximum than bulk CdS and display a zero anisotropy for all excitation wavelengths. Both nanoparticles display strongly heterogeneous intensity decays with mean decay times of 93 ns and 10 &mgr;s for the blue and red emitting particles, respectively. Both types of nanoparticles were several times more photostable upon continous illumination than fluorescein. In spite of the long decay times the nanoparticles are mostly insensitive to dissolved oxygen but are quenched by iodide. These nanoparticles can provide a new class of luminophores for use in chemical sensing, DNA sequencing, high throughput screening and other applications.
摘要翻译：描述了两种新型CdS纳米颗粒和纳米颗粒的稳态和时间分辨发光光谱性质。在胺封端的树枝状大分子存在下形成的CdS纳米颗粒显示蓝色发射。这些纳米颗粒的发射波长取决于激发波长。 CdS /树枝状大分子纳米粒子显示极化发射，各向异性从340到420nm激发逐渐上升，达到超过0.3的最大各向异性值。还描述了来自发光纳米颗粒的新的恒定的正极化发射。描述了多磷酸盐稳定的CdS纳米颗粒，其显示比体积CdS更长的波长红色发射，并且对于所有激发波长显示零各向异性。两种纳米颗粒都显示强烈的非均匀强度衰变，其平均衰减时间分别为93ns和10μs，分别为蓝色和红色发射粒子。两种类型的纳米颗粒在连续照射下比荧光素多几倍的光稳定性。尽管长时间的衰变时间，纳米颗粒对溶解氧大多不敏感，但被碘化物淬灭。这些纳米颗粒可以提供一类新的发光体，用于化学感测，DNA测序，高通量筛选和其他应用。

14. 发明授权

US06395556B1 Polarization based sensing 失效
标题翻译：基于极化的感测
公开(公告)号：US06395556B1
公开(公告)日：2002-05-28
申请号：US09831625
申请日：2001-08-07
申请人： Joseph R. Lakowicz , Ignacy Gryczynski , Zygmunt Gryczynski
发明人： Joseph R. Lakowicz , Ignacy Gryczynski , Zygmunt Gryczynski
IPC分类号： G01N2164
CPC分类号： G01N21/6445 , G01N21/6428 , G01N2021/6441 , Y10T436/144444 , Y10T436/209163
摘要： The present invention relates to the determination of the presence or concentration of an analyte in a sample by visual or electronic element, using polarization based sensing techniques (14) employing fluorescent sensing (11) and reference molecules (10).
摘要翻译：本发明涉及使用采用荧光检测（11）和参考分子（10）的基于偏振的感测技术（14），通过视觉或电子元件确定样品中分析物的存在或浓度。

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