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11. 发明授权

US07667023B2 Promoter and vectors for plant transformation and methods of using same 有权
标题翻译：植物转化的启动子和载体及其使用方法
公开(公告)号：US07667023B2
公开(公告)日：2010-02-23
申请号：US11927974
申请日：2007-10-30
申请人： Bruce Held , Vaithilingam Sekar , Janell Eby , Carol Lewnau , Sumei Xiong , Phil Dykema , Herbert Martin Wilson
发明人： Bruce Held , Vaithilingam Sekar , Janell Eby , Carol Lewnau , Sumei Xiong , Phil Dykema , Herbert Martin Wilson
IPC分类号： C07H21/04 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8216
摘要： The invention is directed to a promoter, designated MuB, sequences which hybridize to same and functional fragments thereof. The regulatory element of the invention provide improved expression in plants of operably linked nucleotide sequences. Expression vectors with the regulatory element is the subject of the invention, which may further include an operably linked nucleotide sequence. The invention is further directed to transformed plant tissue including the nucleotide sequence and to transformed plants and seeds thereof. The regulatory element is useful for driving gene or antisense expression or the like for the purpose of imparting agronomically useful traits such as, but not limited to, increase in yield, disease resistance, insect resistance, herbicide tolerance, drought tolerance and salt tolerance in plants.
摘要翻译：本发明涉及一种名为MuB的启动子，其与其相同和功能片段杂交。本发明的调节元件在植物中提供可操作地连接的核苷酸序列的改进的表达。具有调节元件的表达载体是本发明的主题，其可以进一步包括可操作地连接的核苷酸序列。本发明进一步涉及包含核苷酸序列的转化植物组织以及其转化的植物和种子。调节元件可用于驱动基因或反义表达等，以赋予农学上有用的性状，例如但不限于在植物中增加产量，抗病性，抗虫性，除草剂耐受性，耐旱性和耐盐性。

12. 发明授权

US09540655B2 Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof 有权
标题翻译：堆叠除草剂耐受事件8264.44.06.1，相关转基因大豆品系及其检测
公开(公告)号：US09540655B2
公开(公告)日：2017-01-10
申请号：US13991246
申请日：2011-12-02
申请人： Yunxing Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn Parkhurst , Sandra Toledo , Terry Wright , Sean Russell , Bruce Held , Vaithilingam Sekar
发明人： Yunxing Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn Parkhurst , Sandra Toledo , Terry Wright , Sean Russell , Bruce Held , Vaithilingam Sekar
IPC分类号： C12N15/82 , C12Q1/68 , A01H5/00 , A01H5/10 , A01N57/10 , C12N9/10 , A01G1/00
CPC分类号： A01N39/04 , A01H1/02 , C12N9/1092 , C12N9/93 , C12N15/8241 , C12N15/8274 , C12N15/8275 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/16 , C12Y205/01019 , C12Y604/01002
摘要： This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
摘要翻译：本发明部分涉及大豆事件pDAB8264.44.06.1，并且包括新型表达盒和包含赋予对草甘膦，芳氧基链烷酸酯和草铵膦除草剂的抗性的多种性状的转基因插入物。本发明还部分涉及控制抗性杂草，植物育种和除草剂耐受性植物的方法。在一些实施方案中，事件序列可以与其他性状“堆叠”，包括例如其它除草剂耐受性基因和/或昆虫抑制蛋白。本发明还部分涉及在大豆和相关植物材料中检测事件pDAB8264.44.06.1的终点TaqMan PCR测定。一些实施方案可以进行植物材料的高通量接合度分析，并且可以使用其他实施方案来唯一地鉴定包含本发明事件的大豆品系的接合度和品种。还提供了用于进行这些测定的试剂盒和条件。

13. 发明申请

US20140041083A1 STACKED HERBICIDE TOLERANCE EVENT 8264.44.06.1, RELATED TRANSGENIC SOYBEAN LINES, AND DETECTION THEREOF 有权
标题翻译：相关转基因大豆品种8264.44.06.1的堆肥除草剂耐受性及其检测
公开(公告)号：US20140041083A1
公开(公告)日：2014-02-06
申请号：US13991246
申请日：2011-12-02
申请人： Yunxing Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn Parkhurst , Sandra Toledo , Terry Wright , Sean Russell , Bruce Held , Vaithilingam Sekar
发明人： Yunxing Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn Parkhurst , Sandra Toledo , Terry Wright , Sean Russell , Bruce Held , Vaithilingam Sekar
IPC分类号： C12N15/82 , A01G1/00
CPC分类号： A01N39/04 , A01H1/02 , C12N9/1092 , C12N9/93 , C12N15/8241 , C12N15/8274 , C12N15/8275 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/16 , C12Y205/01019 , C12Y604/01002
摘要： This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
摘要翻译：本发明部分涉及大豆事件pDAB8264.44.06.1，并且包括新型表达盒和包含赋予对草甘膦，芳氧基链烷酸酯和草铵膦除草剂的抗性的多种性状的转基因插入物。本发明还部分涉及控制抗性杂草，植物育种和除草剂耐受性植物的方法。在一些实施方案中，事件序列可以与其他性状“堆叠”，包括例如其它除草剂耐受性基因和/或昆虫抑制蛋白。本发明还部分涉及在大豆和相关植物材料中检测事件pDAB8264.44.06.1的终点TaqMan PCR测定。一些实施方案可以进行植物材料的高通量接合度分析，并且可以使用其他实施方案来唯一地鉴定包含本发明事件的大豆品系的接合度和品种。还提供了用于进行这些测定的试剂盒和条件。

14. 发明申请

US20120240291A1 Regulatory regions preferentially expressing in non-pollen plant tissue 有权
标题翻译：调控区优先在非花粉植物组织中表达
公开(公告)号：US20120240291A1
公开(公告)日：2012-09-20
申请号：US13051358
申请日：2011-03-18
申请人： Bruce Held , Vaithilingam Sekar , Janell Eby , Carol Lewnau , Penny Perkins
发明人： Bruce Held , Vaithilingam Sekar , Janell Eby , Carol Lewnau , Penny Perkins
IPC分类号： C12N15/82 , C07H1/00 , A01H5/00 , A01H5/10 , C07H21/00 , C12N5/10
CPC分类号： C07K14/415 , C12N15/8216
摘要： Regulatory regions are shown which regulate expression of an operably linked heterologous nucleic acid molecule in plants. Promoters are described which express at lower levels in pollen cells that in other plant cells. Methods of using such promoter to regulate expression of an operably linked nucleic acid molecule are described. A polyadenylation nucleotide sequence from soybean is further shown.
摘要翻译：显示调节区域，其调节植物中可操作地连接的异源核酸分子的表达。描述了在其它植物细胞中在花粉细胞中较低水平表达的启动子。描述了使用这种启动子调节可操作地连接的核酸分子的表达的方法。进一步显示了来自大豆的聚腺苷酸化核苷酸序列。

15. 发明申请

US20120144530A1 Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells 审中-公开
标题翻译：优化植物细胞中草甘膦抗性编码核酸分子的表达
公开(公告)号：US20120144530A1
公开(公告)日：2012-06-07
申请号：US13303502
申请日：2011-11-23
申请人： Bruce Held , Vaithilingam Sekar , Terry Wright , Sean Russell
发明人： Bruce Held , Vaithilingam Sekar , Terry Wright , Sean Russell
IPC分类号： A01H5/00 , C12N15/63 , C12Q1/68 , C12N15/82 , A01N57/20 , C12N15/52 , C12N5/10
CPC分类号： C12N9/1092 , C12N15/8275
摘要： A nucleic acid molecule encoding a 5-enolpyruvyl-3-phosphoshikimic acid synthase (EPSPS) that provides resistance to glyphosate is provided, that has been optimized for expression in both monocotyledonous and dicotyledonous plants and preferably in soybeans. Methods of use are also provided.
摘要翻译：提供了编码对草甘膦具有抗性的5-烯醇丙酮酰-3-磷酸嘧啶酸合酶（EPSPS）的核酸分子，其被优化用于在单子叶植物和双子叶植物中优选在大豆中表达。还提供了使用方法。

16. 发明授权

US07393948B1 Ubiquitin regulatory nucleic acids, vectors, and methods of using same 有权
标题翻译：泛素调节核酸，载体及其使用方法
公开(公告)号：US07393948B1
公开(公告)日：2008-07-01
申请号：US11773861
申请日：2007-07-05
申请人： Vaithilingam Sekar , Carol Lewnau , Janell Eby , Brad Atchinson , Bruce Held
发明人： Vaithilingam Sekar , Carol Lewnau , Janell Eby , Brad Atchinson , Bruce Held
IPC分类号： C07H21/04 , C12N15/63 , C12N5/14 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8216
摘要： The invention is directed to a soybean polyubiquitin promoter, polyubiquitin terminator, sequences which hybridize to same and functional fragments thereof. The regulatory element of the invention provide improved expression in plants of operably linked nucleotide sequences. Expression vectors with the regulatory element is the subject of the invention, which may further include an operably linked nucleotide sequence. The invention is further directed to transformed plant tissue including the nucleotide sequence and to transformed plants and seeds thereof. The regulatory element is useful for driving a nucleotide sequence, for example a gene, or antisense expression or the like for the purpose of imparting agronomically useful traits such as, but not limited to, increase in yield, disease resistance, insect resistance, herbicide tolerance, drought tolerance and salt tolerance in plants.
摘要翻译：本发明涉及大豆多聚遍在蛋白启动子，多聚遍在蛋白终止子，与其相同和功能片段杂交的序列。本发明的调节元件在植物中提供可操作地连接的核苷酸序列的改进的表达。具有调节元件的表达载体是本发明的主题，其可以进一步包括可操作地连接的核苷酸序列。本发明进一步涉及包含核苷酸序列的转化植物组织以及其转化的植物和种子。调节元件可用于驱动核苷酸序列，例如基因或反义表达等，以赋予农学上有用的性状，例如但不限于产量增加，抗病性，抗虫性，除草剂耐受性，耐旱性和耐盐性。

17. 发明授权

US09732353B2 Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof 有权
公开(公告)号：US09732353B2
公开(公告)日：2017-08-15
申请号：US13548720
申请日：2012-07-13
申请人： Thomas Hoffman , Ning Zhou , Dayakar Pareddy , Yunxing Cory Cui , Dawn Marie Parkhurst , Nathan Bard , Sandra Grace Toledo , Gregory Alan Bradfisch , Bruce Held , Vaithilingam Sekar , Lauren Clark , Sean Michael Russell , Kelly Ann Smith , Yang Wang , Terry R. Wright
发明人： Thomas Hoffman , Ning Zhou , Dayakar Pareddy , Yunxing Cory Cui , Dawn Marie Parkhurst , Nathan Bard , Sandra Grace Toledo , Gregory Alan Bradfisch , Bruce Held , Vaithilingam Sekar , Lauren Clark , Sean Michael Russell , Kelly Ann Smith , Yang Wang , Terry R. Wright
IPC分类号： C12N15/82 , A01H5/10
CPC分类号： C12N15/8274 , A01H5/10 , C12N15/8275 , C12N15/8277 , C12Y114/11017 , C12Y203/01183 , C12Y205/01019
摘要： This invention relates to soybean event pDAB8264.42.32.1 and includes novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TAQMAN PCR assays for the detection of Event pDAB8264.42.32.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

18. 发明授权

US07888561B2 Ubiquitin regulatory nucleic acids, vectors, and methods of using same 有权
标题翻译：泛素调节核酸，载体及其使用方法
公开(公告)号：US07888561B2
公开(公告)日：2011-02-15
申请号：US12480275
申请日：2009-06-08
申请人： Vaithilingam Sekar , Carol Lewnau , Janell Eby , Brad Atchinson , Bruce Held
发明人： Vaithilingam Sekar , Carol Lewnau , Janell Eby , Brad Atchinson , Bruce Held
IPC分类号： A01H5/00 , C12N15/82 , C12N15/63 , C12N5/14 , C07H21/04
CPC分类号： C12N15/8216
摘要： The invention is directed to a soybean polyubiquitin promoter, polyubiquitin terminator, sequences which hybridize to same and functional fragments thereof. The regulatory element of the invention provide improved expression in plants of operably linked nucleotide sequences. Expression vectors with the regulatory element is the subject of the invention, which may further include an operably linked nucleotide sequence. The invention is further directed to transformed plant tissue including the nucleotide sequence and to transformed plants and seeds thereof. The regulatory element is useful for driving a nucleotide sequence, for example a gene, or antisense expression or the like for the purpose of imparting agronomically useful traits such as, but not limited to, increase in yield, disease resistance, insect resistance, herbicide tolerance, drought tolerance and salt tolerance in plants.
摘要翻译：本发明涉及大豆多聚遍在蛋白启动子，多聚遍在蛋白终止子，与其相同和功能片段杂交的序列。本发明的调节元件在植物中提供可操作地连接的核苷酸序列的改进的表达。具有调节元件的表达载体是本发明的主题，其可以进一步包括可操作地连接的核苷酸序列。本发明进一步涉及包含核苷酸序列的转化植物组织以及其转化的植物和种子。调节元件可用于驱动核苷酸序列，例如基因或反义表达等，以赋予农学上有用的性状，例如但不限于增加产量，抗病性，抗虫性，除草剂耐受性，耐旱性和耐盐性。

19. 发明申请

US20100199377A1 Ubiquitin regulatory nucleic acids, vectors, and methods of using same 有权
标题翻译：泛素调节核酸，载体及其使用方法
公开(公告)号：US20100199377A1
公开(公告)日：2010-08-05
申请号：US12480275
申请日：2009-06-08
申请人： Vaithilingam Sekar , Carol Lewnau , Janell Eby , Brad Atchinson , Bruce Held
发明人： Vaithilingam Sekar , Carol Lewnau , Janell Eby , Brad Atchinson , Bruce Held
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04 , C12N15/63 , C12N5/10
CPC分类号： C12N15/8216
摘要： The invention is directed to a soybean polyubiquitin promoter, polyubiquitin terminator, sequences which hybridize to same and functional fragments thereof. The regulatory element of the invention provide improved expression in plants of operably linked nucleotide sequences. Expression vectors with the regulatory element is the subject of the invention, which may further include an operably linked nucleotide sequence. The invention is further directed to transformed plant tissue including the nucleotide sequence and to transformed plants and seeds thereof. The regulatory element is useful for driving a nucleotide sequence, for example a gene, or antisense expression or the like for the purpose of imparting agronomically useful traits such as, but not limited to, increase in yield, disease resistance, insect resistance, herbicide tolerance, drought tolerance and salt tolerance in plants.
摘要翻译：本发明涉及大豆多聚遍在蛋白启动子，多聚遍在蛋白终止子，与其相同和功能片段杂交的序列。本发明的调节元件在植物中提供可操作地连接的核苷酸序列的改进的表达。具有调节元件的表达载体是本发明的主题，其可以进一步包括可操作地连接的核苷酸序列。本发明进一步涉及包含核苷酸序列的转化植物组织以及其转化的植物和种子。调节元件可用于驱动核苷酸序列，例如基因或反义表达等，以赋予农学上有用的性状，例如但不限于增加产量，抗病性，抗虫性，除草剂耐受性，耐旱性和耐盐性。

20. 发明申请

US20090220999A1 Antibodies immunoreactive with mutant 5-enolpyruvlshikimate-3-phosphate synthase 有权
标题翻译：抗体与突变体5-烯醇丙酮酸莽草酸-3-磷酸合酶的免疫反应
公开(公告)号：US20090220999A1
公开(公告)日：2009-09-03
申请号：US12212048
申请日：2008-09-17
申请人： Vaithilingam Sekar , Bruce Held , Kyu Chung , Paul F. Russell, JR.
发明人： Vaithilingam Sekar , Bruce Held , Kyu Chung , Paul F. Russell, JR.
IPC分类号： G01N33/567 , C07K16/00 , C12N15/11 , C12N5/12
CPC分类号： G01N33/573 , C07K16/40
摘要： Antibodies immunoreactive to double mutant EPSPS are provided, and in an embodiment the double mutant EPSPS is one in which the wild-type EPSPS is substituted at residue 102 with isoleucine and at residue 106 with serine. Also provided are hybridomas producing the antibodies, as well as methods of making and using the antibodies.
摘要翻译：提供了对双突变EPSPS免疫反应的抗体，在一个实施方案中，双突变体EPSPS是其中野生型EPSPS在残基102与异亮氨酸取代的抗体，而残基106与丝氨酸取代。还提供了产生抗体的杂交瘤，以及制备和使用抗体的方法。

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