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11. 发明申请

US20110065153A1 METHOD OF PRODUCING L-LYSINE 有权
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US20110065153A1
公开(公告)日：2011-03-17
申请号：US12915793
申请日：2010-10-29
申请人： SEIKO OTSUNA , MASAKAZU SUGIMOTO , MASAKO IZUI , ATSUSHI HAYAKAWA , EIICHI NAKANO , MASAKI KOBAYASHI , YASUHIKO YOSHIHARA , TSUYOSHI NAKAMATSU
发明人： SEIKO OTSUNA , MASAKAZU SUGIMOTO , MASAKO IZUI , ATSUSHI HAYAKAWA , EIICHI NAKANO , MASAKI KOBAYASHI , YASUHIKO YOSHIHARA , TSUYOSHI NAKAMATSU
IPC分类号： C12P13/08
CPC分类号： C12N9/0016 , C12N9/1217 , C12N9/88 , C12N15/52 , C12N15/77 , C12P13/08
摘要： The ability and speed with which a coryneform bacterium can produce L-lysine are improved when the coryneform bacterium contains an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized. This is accomplished by successively enhancing the DNA coding for dihydrodipicolinate reductase, the DNA coding for dihydrodipicolinate synthase, the DNA coding for diaminopimelate decarboxylase, and the DNA coding for diaminopimelate dehydrogenase.
摘要翻译：当棒状细菌含有其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏的天冬氨酸激酶时，棒状细菌可以产生L-赖氨酸的能力和速度得到改善。这是通过连续增强编码二氢吡啶二羧酸还原酶的DNA，编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA来实现的。

12. 发明授权

US07858348B2 N-acetyl-(R,S)-beta-amino acid acylase gene 有权
标题翻译： N-乙酰基 - （R，S） - 氨基酸酰基转移酶基因
公开(公告)号：US07858348B2
公开(公告)日：2010-12-28
申请号：US11782260
申请日：2007-07-24
申请人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
发明人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04
CPC分类号： C12N9/80
摘要： The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.
摘要翻译：本发明提供编码N-乙酰基 - （R，S） - 氨基酸酰基转移酶的基因。从细菌细胞中分离纯化N-乙酰基 - （R，S） - 氨基酸酰基转移酶，测定核苷酸序列。宿主如大肠杆菌用于构建这些基因的高表达系统。由伯克霍尔德氏菌属（Burkholderia sp。）生产的N-乙酰基 - （R） - AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。由伯克霍尔德氏菌属（Burkholderia sp。）生产的N-乙酰基 - （S） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 9.由Variovorax sp。生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110348（FERM BP-10367）包括例如由SEQ ID NO：1所示的氨基酸序列组成的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 11。

13. 发明授权

US07608437B2 Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
标题翻译：构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法
公开(公告)号：US07608437B2
公开(公告)日：2009-10-27
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/14 , C12Q1/48 , C12Q1/32 , C12Q1/68 , C12N9/02 , C12N9/10 , C12N1/21 , C12P21/00 , C12N15/00 , C07K14/00 , C07H21/00
CPC分类号： C12P13/14 , C12N15/67
摘要： A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.
摘要翻译：具有改进的氨基酸或核酸生产力的棒状杆菌细菌的方法包括以下步骤：将氨基酸或核酸 - 生物合成基因的启动子序列中的突变引入棒状细菌的染色体上，使其接近共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使得其接近共有序列，获得棒状杆菌型氨基酸的突变体或产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。该方法允许本领域技术人员通过重组或突变来构建能够富集或控制预期基因的表达而不使用质粒并以高产率促进氨基酸产生的突变体。

14. 发明申请

US20080241895A1 N-Acetyl-(R,S)-beta-Amino Acid Acylase Gene 有权
标题翻译： N-乙酰基 - （R，S） - 氨基酸酰基酶
公开(公告)号：US20080241895A1
公开(公告)日：2008-10-02
申请号：US11782260
申请日：2007-07-24
申请人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
发明人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C07H21/04 , C12N9/80 , C12N1/21
CPC分类号： C12N9/80
摘要： The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.
摘要翻译：本发明提供编码N-乙酰基 - （R，S） - 氨基酸酰基转移酶的基因。从细菌细胞中分离纯化N-乙酰基 - （R，S） - 氨基酸酰基转移酶，确定核苷酸序列。宿主如大肠杆菌用于构建这些基因的高表达系统。伯克霍尔德氏菌属生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。由伯克霍尔德氏菌属生产的N-乙酰基 - （S） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 9.由Variovorax sp。生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110348（FERM BP-10367）包括例如由SEQ ID NO：1所示的氨基酸序列组成的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 11。

15. 发明申请

US20050095680A1 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
公开(公告)号：US20050095680A1
公开(公告)日：2005-05-05
申请号：US11011701
申请日：2004-12-15
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12N1/21 , C12N9/00 , C12P13/10 , C07H21/04 , C12N9/12
CPC分类号： C12N9/93 , C12P13/10
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

16. 发明授权

US06338956B1 Stress-resistant microorganism and method of producing fermentative product 有权
标题翻译：耐应力微生物和发酵产物的生产方法
公开(公告)号：US06338956B1
公开(公告)日：2002-01-15
申请号：US09634632
申请日：2000-08-08
申请人： Eiichiro Kimura , Yoshimi Kikuchi , Yoshio Kawahara , Shinya Goto , Osamu Kurahashi , Tsuyoshi Nakamatsu
发明人： Eiichiro Kimura , Yoshimi Kikuchi , Yoshio Kawahara , Shinya Goto , Osamu Kurahashi , Tsuyoshi Nakamatsu
IPC分类号： C12P1304
CPC分类号： C12P13/222 , C12P13/04 , C12P13/08 , C12P13/14
摘要： A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a &sgr; factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.
摘要翻译：利用微生物通过在培养基中培养微生物发酵生产有用物质如氨基酸，使发酵产物在培养基中产生和积累，收集发酵产物，其中所用的微生物通过引入进行修饰编码热休克蛋白的基因中的至少一种和编码特异性用于热休克蛋白基因的西格玛因子的基因以增强细胞中的热休克蛋白的表达量，由此允许微生物添加否则会抑制微生物的生长和/或发酵产物的产生。

17. 发明授权

US5977331A .alpha.-Ketoglutarate dehydrogenase gene 失效
标题翻译： α-酮戊二酸脱氢酶基因
公开(公告)号：US5977331A
公开(公告)日：1999-11-02
申请号：US750152
申请日：1996-12-12
申请人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N1/21 , C12N9/02 , C12N15/53 , C12P13/08 , C12P13/14 , C07H21/02 , C12N1/20 , C12P13/04
CPC分类号： C12N9/0008 , C12P13/08 , C12P13/14
摘要： Disclosed are coryneform L-glutamic acid-producing bacteria deficient in .alpha.-ketoglutarate dehydrogenase, a method of producing L-glutamic acid by using the bacteria, a gene coding for an enzyme having .alpha.-KGDH activity originating from coryneform L-glutamic acid-producing bacteria, recombinant DNA containing the gene, coryneform bacteria harboring the recombinant DNA, and a method of producing L-lysine by using bacteria harboring the recombinant DNA and having L-lysine productivity.
摘要翻译： PCT No.PCT / JP95 / 01131 Sec。 371日期：1996年12月12日 102（e）日期1996年12月12日PCT提交1995年6月7日PCT公布。 WO95 / 34672 PCT公开号日期1995年12月21日公开是α-酮戊二酸脱氢酶缺乏的棒状L-谷氨酸生产菌，使用细菌生产L-谷氨酸的方法，编码具有源自棒状杆菌L的α-KGDH活性的酶的基因谷氨酸生产菌，含有该基因的重组DNA，携带重组DNA的棒状细菌，以及通过使用含有重组DNA并具有L-赖氨酸生产力的细菌产生L-赖氨酸的方法。

18. 发明授权

US5616480A Temperature sensitive plasmid 失效
标题翻译：温度敏感质粒
公开(公告)号：US5616480A
公开(公告)日：1997-04-01
申请号：US408188
申请日：1995-03-22
申请人： Masakazu Sugimoto , Hiroyuki Kojima , Akiko Tanaka , Hiroshi Matsui , Katsuaki Sato , Tsuyoshi Nakamatsu
发明人： Masakazu Sugimoto , Hiroyuki Kojima , Akiko Tanaka , Hiroshi Matsui , Katsuaki Sato , Tsuyoshi Nakamatsu
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12N9/12 , C12N15/63 , C12N15/77 , C12P13/08 , C12P13/22 , C12R1/13 , C12N1/20
CPC分类号： C12N9/00 , C12N15/77 , C12N9/12 , C12P13/08 , C12P13/22
摘要： A plasmid having a temperature-sensitive replication origin which has the following properties:a) capable of autonomous replication in Corynebacterium and being retained in Corynebacterium; and,b) when a cell containing said plasmid is cultured at 31.degree. to 37.degree. C., replication of the plasmid is inhibited and at the same time, the plasmid is removed from the cell body; and a method for performing homologous recombination in Corynebacterium using the plasmid.
摘要翻译：具有温度敏感复制起点的质粒，其具有以下性质：a）能够在棒状杆菌中自主复制并保留在棒状杆菌中; 和b）当含有所述质粒的细胞在31℃至37℃下培养时，质粒的复制被抑制，同时从细胞体中除去质粒; 以及使用该质粒在棒状杆菌中进行同源重组的方法。

19. 发明授权

US5405628A Feed additive composition for ruminants 失效
标题翻译：反刍动物饲料添加剂组合物
公开(公告)号：US5405628A
公开(公告)日：1995-04-11
申请号：US122656
申请日：1993-09-17
申请人： Satoshi Ueda , Haruo Heima , Makoto Ozawa , Takeshi Nagai , Tsuyoshi Nakamatsu , Hiroyuki Sato
发明人： Satoshi Ueda , Haruo Heima , Makoto Ozawa , Takeshi Nagai , Tsuyoshi Nakamatsu , Hiroyuki Sato
IPC分类号： A23K1/16 , A23K1/00 , A23K1/18 , A61K9/50
CPC分类号： A61K9/5015 , A23K40/35 , A61K9/5031 , Y10S426/807
摘要： A feed additive composition is provided having a core which contains a biologically active substance and which is coated with a coating composition which contains components a) and b):a) at least one substance selected from the group consisting of linear or branched saturated or unsaturated aliphatic monocarboxylic acids of 12-22 carbon atoms or salts thereof, hardened vegetable fats and oils, hardened animal fats and oils, and wax; andb) at least one of substances i) and ii):i) one or more linear or branched saturated or unsaturated aliphatic monocarboxylic acids of 11 carbon atoms or less, andii) one or more substances selected from the group consisting of nucleic acids, nucleotides, nucleosides, bases composing nucleic acids and salts thereof.
摘要翻译：提供了一种饲料添加剂组合物，其具有含有生物活性物质的核心，并且涂覆有包含组分a）和b）的涂料组合物：a）至少一种选自直链或支链饱和或不饱和的 12-22个碳原子的脂肪族单羧酸或其盐，硬化植物油脂，硬化动物脂肪和油和蜡; 和b）物质i）和ii）中的至少一种：i）一个或多个11个碳原子或更少的直链或支链饱和或不饱和脂族单羧酸，和ii）一种或多种选自核酸，核苷酸，核苷，构成核酸及其盐的碱。

20. 发明授权

US5177013A Preparation of an immobilized lipase having a low water content without drying 失效
标题翻译：制备具有低水分而不干燥的固定化脂肪酶
公开(公告)号：US5177013A
公开(公告)日：1993-01-05
申请号：US554853
申请日：1990-07-20
申请人： Naoki Usui , Naoto Kato , Tsuyoshi Nakamatsu , Jun Kurashige
发明人： Naoki Usui , Naoto Kato , Tsuyoshi Nakamatsu , Jun Kurashige
IPC分类号： C12P7/64 , C12N9/96 , C12N11/00 , C12N11/02 , C12N11/08 , C12N11/10 , C12N11/14
CPC分类号： C12N11/00 , C12N11/08 , C12N11/14 , C12N9/96
摘要： Lipase is absorbed on a carrier to provide an immobilized lipase preparation containing 0.5 to 8% water by a process that does not require drying. The process is carried out by providing a solution or dispersion of lipase in a liquid solvent, mixing the solution or dispersion of lipase with a carrier to provide a mixture containing 0.5 to 8% water and kneading the mixture to produce the immobilized lipase preparation. The solution or dispersion of lipase may contain an enzyme activity enhancing agent such as a fatty acid or its derivative such as lecithin. By not drying, an immobilized lipase preparation having higher activity is obtained.
摘要翻译：脂肪酶被吸收在载体上以通过不需要干燥的方法提供含有0.5至8％水的固定化脂肪酶制剂。该方法通过在液体溶剂中提供脂肪酶的溶液或分散体，将脂肪酶的溶液或分散体与载体混合以提供含有0.5至8％的水的混合物并捏合该混合物以产生固定的脂肪酶制剂。脂肪酶的溶液或分散体可含有酶活性增强剂如脂肪酸或其衍生物如卵磷脂。通过不干燥，得到具有较高活性的固定化脂肪酶制剂。

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