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    • 13. 发明授权
    • Modified Cry35 proteins
    • 修饰的Cry35蛋白
    • US07939651B2
    • 2011-05-10
    • US12715744
    • 2010-03-02
    • H. Ernest SchnepfKenneth E. NarvaSteven L. Evans
    • H. Ernest SchnepfKenneth E. NarvaSteven L. Evans
    • C07H19/16C07K14/00C12N15/09
    • C07K14/325
    • This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.
    • 本发明提供与野生型Cry35蛋白相比具有增强性质的改良的杀虫Cry35蛋白。 对这些蛋白质的修饰部分基于Cry35类中〜45kDa 149B1蛋白质和其他蛋白质的原子坐标和三维(3D)结构的分析。 本发明还包括编码这些修饰蛋白质的多核苷酸,以及产生这些修饰蛋白质的转基因植物。 本发明还提供了用这些修饰蛋白质控制植物害虫(包括根虫)的方法。 本发明的修饰蛋白包括涉及交换的片段,结构域和基序的嵌合毒素,如本文所讨论的。 本发明还提供了修饰Cry35蛋白的方法。
    • 14. 发明授权
    • Modified Cry35 proteins having altered protease stability
    • 具有改变的蛋白酶稳定性的修饰的Cry35蛋白
    • US07737263B2
    • 2010-06-15
    • US11943814
    • 2007-11-21
    • H. Ernest SchnepfKenneth E. NarvaSteven L. Evans
    • H. Ernest SchnepfKenneth E. NarvaSteven L. Evans
    • C07H19/16C07K14/00C12N15/09
    • C07K14/325
    • This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.
    • 本发明提供与野生型Cry35蛋白相比具有增强性质的改良的杀虫Cry35蛋白。 对这些蛋白质的修饰部分基于Cry35类中〜45kDa 149B1蛋白质和其他蛋白质的原子坐标和三维(3D)结构的分析。 本发明还包括编码这些修饰蛋白质的多核苷酸,以及产生这些修饰蛋白质的转基因植物。 本发明还提供了用这些修饰蛋白质控制植物害虫(包括根虫)的方法。 本发明的修饰蛋白包括涉及交换的片段,结构域和基序的嵌合毒素,如本文所讨论的。 本发明还提供了修饰Cry35蛋白的方法。