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11. 发明申请

WO2006065597A2 ID-TAG COMPLEXES, ARRAYS, AND METHODS OF USE THEREOF 审中-公开
标题翻译： ID-TAG复合体，阵列及其使用方法
公开(公告)号：WO2006065597A2
公开(公告)日：2006-06-22
申请号：PCT/US2005/044269
申请日：2005-12-06
申请人： APPLERA CORPORATION , SCHROTH, Gary, P. , LAO, Kai, Qin , STRAUS, Neil, A.
发明人： SCHROTH, Gary, P. , LAO, Kai, Qin , STRAUS, Neil, A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6816 , C12Q2565/519 , C12Q2537/1373 , C12Q2537/125 , C12Q2565/501 , C12Q2563/131 , C12Q2525/197 , C12Q2563/185
摘要： The present invention relates to the detection of target sequences. Detection can be achieved through the use of ID-tag complexes. These ID-tag complexes are relatively stable in the absence of a target sequence. In the presence of a target sequence, the complexes dissociate and form new complexes or duplexes, which can be purified or eliminated and detected on an ID-tag system.
摘要翻译：本发明涉及靶序列的检测。可以通过使用ID-标签复合物实现检测。这些ID-标签复合物在没有靶序列的情况下是相对稳定的。在靶序列存在下，复合物解离并形成新的复合物或双链体，其可以在ID-标签系统上被纯化或消除并检测。

12. 发明申请

WO2009067632A1 METHOD OF SEQUENCING NUCLEIC ACIDS USING ELABORATED NUCLEOTIDE PHOSPHOROTHIOLATE COMPOUNDS 审中-公开
标题翻译：使用制备的核酸磷酸酯化合物测序核酸的方法
公开(公告)号：WO2009067632A1
公开(公告)日：2009-05-28
申请号：PCT/US2008/084248
申请日：2008-11-20
申请人： APPLIED BIOSYSTEMS INC. , LAO, Kai Q. , STRAUS, Neil A.
发明人： LAO, Kai Q. , STRAUS, Neil A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883
摘要： The present teachings provide methods, compositions, and kits for synthesizing and sequencing nucleic acids. In some embodiments, elaborated nucleotide phosphorothiolate compounds are employed along with efficient cleaving reactions. Improved sequencing efficiency is achieved by the rapid polymerase-mediated incorporation of elaborated nucleotide phosphorothiolate compounds. Increased sequencing efficiency is also achieved by the ability of the cleaving reactions to restore the incorporated nucleotides to their natural structure prior to subsequent elongation.
摘要翻译：本教导提供了用于合成和测序核酸的方法，组合物和试剂盒。在一些实施方案中，精制的核苷酸硫代磷酸酯化合物与有效的切割反应一起使用。通过快速聚合酶介导的拟合的核苷酸硫代硫酸化合物的掺入可以提高测序效率。增加的测序效率也可以通过切割反应在随后的伸长之前将掺入的核苷酸恢复到其天然结构的能力来实现。

13. 发明申请

WO2007044066A3 METHODS FOR NORMALIZING AND FOR IDENTIFYING SMALL NUCLEIC ACIDS 审中-公开
标题翻译：用于正常化和鉴定小核酸的方法
公开(公告)号：WO2007044066A3
公开(公告)日：2008-01-10
申请号：PCT/US2006012025
申请日：2006-03-29
申请人： APPLERA CORP , LAO KAI QIN , STRAUS NEIL A , BURNS JOHN W
发明人： LAO KAI QIN , STRAUS NEIL A , BURNS JOHN W
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6855 , C12Q1/6851 , C12Q2535/138 , C12Q2525/121 , C12Q2525/301
摘要： The present teachings are generally directed to methods for normalizing at least one species of small nucleic acid that is present in a population of small nucleic acid species, wherein the relative concentration of at least one small nucleic acid species is substantially greater than the relative concentration of at least one other small nucleic acid species in the population. At least one small nucleic acid species is normalized using a multiplicity of primers comprising degenerate sequences. In some embodiments, a small nucleic acid species is identified by inserting at least part of an extension product from a normalized population into a vector and subsequently sequencing the insert. In some embodiments, a small nucleic acid species is identified by determining the sequence of at least part of an extension product.
摘要翻译：本教导通常涉及用于归一化存在于小核酸物种群中的至少一种小核酸的方法，其中至少一种小核酸物质的相对浓度基本上大于群体中至少一种其他小核酸物种。使用包含简并序列的多个引物将至少一个小核酸物质归一化。在一些实施方案中，通过将来自标准化群体的至少部分延伸产物插入载体并随后对插入物进行测序来鉴定小核酸物种。在一些实施方案中，通过确定延伸产物的至少一部分的序列来鉴定小核酸物种。

14. 发明申请

WO2007011903A2 ANALYZING MESSENGER RNA AND MICRO RNA IN THE SAME REACTION MIXTURE 审中-公开
标题翻译：在相同的反应混合物中分析信使RNA和微RNA
公开(公告)号：WO2007011903A2
公开(公告)日：2007-01-25
申请号：PCT/US2006/027757
申请日：2006-07-17
申请人： APPLERA CORPORATION , LAO, Kai, Qin , STRAUS, Neil, A.
发明人： LAO, Kai, Qin , STRAUS, Neil, A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/101 , C12Q2537/143 , C12Q2527/107 , C12Q2525/301 , C12Q2525/207 , C12Q2533/101
摘要： The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature.
摘要翻译：本教导提供用于在相同反应混合物中的至少两个靶多核苷酸上进行引物延伸反应的方法，组合物和试剂盒。在一些实施方案中，对具有包含自身互补茎和环的热起始底物的第一靶多核苷酸进行逆转录反应，并且在高温下形成延伸产物，但延伸产物在低温下形成较少，热启动引物的互补干扰物可以防止目标特异性区域与靶标的杂交。然而，具有游离目标特异性区域的非热启动引物可以在低温下与其相应的靶标杂交，并且延伸可以在低温下发生。

15. 发明申请

WO2008076842A3 SEQUENCING METHODS 审中-公开
标题翻译：序列方法
公开(公告)号：WO2008076842A3
公开(公告)日：2008-11-27
申请号：PCT/US2007087472
申请日：2007-12-13
申请人： APPLERA CORP , LAO KAI QIN , STRAUS NEIL A
发明人： LAO KAI QIN , STRAUS NEIL A
IPC分类号： C12P19/34 , C07H21/04 , C12M1/34 , C12M1/40 , C12N11/18 , C12Q1/66 , C12Q1/68
CPC分类号： C12Q1/6872 , C12Q2537/143 , C12Q2535/101
摘要： The present teachings provide methods and compositions for sequencing one or more target nucleic acids. High levels of multiplexing are provided by the use of an emulsion PCR comprising primer-immobilized beads. The resulting reaction products can be sequenced by any of a variety of mobility-dependent analytical techniques, such as mass spectrometry. In some embodiments, a first collection of amplification products on a first collection of beads are transferred to a second collection of beads. In some embodiments, a first collection of amplification products on a first collection of beads is amplified in a rolling circle amplification reaction. The present teachings also provide compositions, kits, and devices for performing and sequencing the products of the emulsion amplification reactions as described herein.
摘要翻译：本教导提供了用于测序一种或多种靶核酸的方法和组合物。通过使用包含引物固定的珠粒的乳液PCR提供了高水平的复用。所得到的反应产物可以通过各种迁移依赖性分析技术如质谱法中的任何一种进行测序。在一些实施方案中，将第一集合珠上的扩增产物的第一集合转移到第二集合珠。在一些实施方案中，在滚动圆扩增反应中扩增第一集合珠上的扩增产物的第一集合。本教导还提供用于对本文所述的乳液扩增反应的产物进行和测序的组合物，试剂盒和装置。

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