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11. 发明申请

US20140174926A1 MOLECULAR DIAGNOSTICS PLATFORM 审中-公开
标题翻译：分子诊断平台
公开(公告)号：US20140174926A1
公开(公告)日：2014-06-26
申请号：US14115215
申请日：2012-05-01
申请人： Donovan Bort , Carrie Graham , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Vijay Srinivasan
发明人： Donovan Bort , Carrie Graham , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Vijay Srinivasan
IPC分类号： G01N27/447 , B81B1/00
CPC分类号： G01N27/44791 , B01F1/0027 , B01F13/0071 , B01F13/0076 , B01F2001/0055 , B01F2215/0037 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/0668 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2300/089 , B01L2400/0406 , B01L2400/0418 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B81B1/004
摘要： A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first “U” shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second “U” shape which is substantially opposite the first “U” shape.
摘要翻译：一种混合液滴的方法，所述方法包括在表面上提供液滴，将所述液滴形成为具有底部区域和两个末端的第一“U”形状，并同时合并末端并在底部区域分离液滴以形成与第一“U”形大体相反的第二“U”形状。

12. 发明授权

US08658111B2 Droplet actuators, modified fluids and methods 有权
标题翻译：液滴致动器，改进的流体和方法
公开(公告)号：US08658111B2
公开(公告)日：2014-02-25
申请号：US13031760
申请日：2011-02-22
申请人： Vijay Srinivasan , Vamsee Pamula , Ramakrishna Sista , Arjun Sudarsan , Prasanna Thwar
发明人： Vijay Srinivasan , Vamsee Pamula , Ramakrishna Sista , Arjun Sudarsan , Prasanna Thwar
IPC分类号： B01L3/00 , B01D57/02
CPC分类号： B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , G01N2035/1046
摘要： The present invention provides droplet actuators, modified fluids and methods relating to droplet operations. An aspect includes a droplet actuator including a droplet operations substrate; an oil based filler fluid on the droplet operations substrate comprising an oil soluble additive in the filler fluid; and a droplet in contact with the oil based filler fluid. Still other aspects are provided.
摘要翻译：本发明提供了液滴致动器，改进的流体和与液滴操作有关的方法。一个方面包括具有液滴操作基板的液滴致动器; 在所述液滴操作基底上的油基填充剂流体，其包含在所述填充流体中的油溶性添加剂; 以及与油基填充液接触的液滴。还提供了其他方面。

13. 发明授权

US08637324B2 Bead incubation and washing on a droplet actuator 有权
标题翻译：珠子孵化并在液滴致动器上进行洗涤
公开(公告)号：US08637324B2
公开(公告)日：2014-01-28
申请号：US13081927
申请日：2011-04-07
申请人： Michael G. Pollack , Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
发明人： Michael G. Pollack , Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
IPC分类号： G01N15/06
CPC分类号： G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
摘要翻译：本发明涉及在液滴致动器上的珠孵化和洗涤。提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。

14. 发明申请

US20120261264A1 Droplet Operations Device 审中-公开
标题翻译：液滴操作装置
公开(公告)号：US20120261264A1
公开(公告)日：2012-10-18
申请号：US13003765
申请日：2009-07-20
申请人： Vijay Srinivasan , Arjun Sudarsan , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Raymond Kozikowski , Nicole Bell
发明人： Vijay Srinivasan , Arjun Sudarsan , Vamsee Pamula , Michael Pollack , Ramakrishna Sista , Raymond Kozikowski , Nicole Bell
IPC分类号： C25B9/00 , B05D1/36 , B05D5/12
CPC分类号： G01N27/44756 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2400/0427 , B01L2400/0688 , B01L2400/086 , B29C59/02 , B41J2/1601 , B81B7/0061 , B81B2201/058 , B81B2201/13 , F04B17/00 , F04B19/006
摘要： The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.
摘要翻译：本发明提供了液滴致动器，其具有用于操纵液滴的液滴操作表面，例如通过进行液滴操作。液滴操作表面通常暴露于液滴操作间隙。液滴操作表面的一个或多个区域可以包括图案化的地形特征。本发明还提供一种液滴致动器，其中使用可去除的膜形成一个或两个面向间隙的液滴操作表面。在各种实施例中，可除去膜可以包括通常与液滴致动器基底（例如电介质层和电极）相关联的其它部件。此外，本发明提供了液滴致动器装置和用于联接和/或密封液滴致动器的基板的方法，例如用于液滴致动器基板的自对准组装的技术。本发明提供了液滴致动器和拆卸液滴致动器的方法，以便为液滴致动器表面的清洁和/或回收提供通路。

15. 发明申请

US20110203930A1 Bead Incubation and Washing on a Droplet Actuator 有权
标题翻译：珠子孵化和滴水致动器洗涤
公开(公告)号：US20110203930A1
公开(公告)日：2011-08-25
申请号：US13081927
申请日：2011-04-07
申请人： Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
发明人： Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
IPC分类号： B03C1/00
CPC分类号： G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
摘要翻译：本发明涉及在液滴致动器上的珠孵化和洗涤。提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。

16. 发明申请

US20110104747A1 Method of Concentrating Beads in a Droplet 有权
标题翻译：浓缩珠子在水滴中的方法
公开(公告)号：US20110104747A1
公开(公告)日：2011-05-05
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
IPC分类号： G01N1/40
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
摘要翻译：提供了将珠子集中在流体装置上的液滴和/或负载珠粒中的方法，其中包括将珠粒集中在液滴中的方法，所述方法包括：（a）提供液滴致动器，其包括：（i）内部液滴操作量; 和（ii）内部容积外部的储存器; （iii）建立在从储存器延伸到内部容积中的液体路径中的液滴; （b）在储存器中的液滴部分中提供磁响应珠; （c）通过所述液体路径将所述磁响应珠磁吸引到位于所述内部容积中的所述液滴的所述部分中; 和（d）在内部容积中形成包含一个或多个磁响应珠的液滴。

17. 发明申请

US20110091989A1 Method of Reducing Liquid Volume Surrounding Beads 有权
标题翻译：减少液体体积环绕珠的方法
公开(公告)号：US20110091989A1
公开(公告)日：2011-04-21
申请号：US12992939
申请日：2009-05-18
申请人： Ramakrishna Sista , Vamsee Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar
发明人： Ramakrishna Sista , Vamsee Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar
IPC分类号： G01N1/40
CPC分类号： B01L3/502792 , B01L3/502761 , B01L2200/0673 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/089 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , G01N1/4077 , G01N35/0098 , G01N2035/00564 , Y10T436/25
摘要： The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
摘要翻译：本发明提供了液滴致动器和液滴致动器技术。除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。本发明方法的输出可以是准备用于执行测定方案的珠粒。本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠粒的乳液的方法，加载珠粒的方法液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。

18. 发明申请

US20110076692A1 Detection of Cardiac Markers on a Droplet Actuator 有权
标题翻译：检测滴液致动器上的心脏标志物
公开(公告)号：US20110076692A1
公开(公告)日：2011-03-31
申请号：US12569725
申请日：2009-09-29
申请人： Ramakrishna Sista , Vamsee K. Pamula , Arjun Sudarsan
发明人： Ramakrishna Sista , Vamsee K. Pamula , Arjun Sudarsan
IPC分类号： G01N33/573 , G01N33/53
CPC分类号： G01N33/6893 , G01N33/54366 , G01N2333/4712 , G01N2333/58 , G01N2800/32 , Y10T436/25
摘要： The present invention provides for the detection of cardiac markers on a droplet actuator. An aspect provides a method of assaying a cardiac marker in a biological sample from a subject, the method including providing a droplet actuator, loading the biological sample and assay reagents on the droplet actuator, executing droplet operations to create sample droplets from the sample and reagent droplets from the reagents on the droplet actuator, and executing droplet operations using the sample droplets and reagent droplets to produce a detectable signal indicative of the quantity of the cardiac marker in the biological sample. Still other aspects are provided.
摘要翻译：本发明提供了在液滴致动器上检测心脏标志物。一方面提供了从受试者的生物样品中测定心脏标志物的方法，所述方法包括提供液滴致动器，在液滴致动器上装载生物样品和测定试剂，执行液滴操作以从样品和试剂产生样品液滴来自液滴致动器上的试剂的液滴，以及使用样品液滴和试剂液滴执行液滴操作，以产生指示生物样品中心脏标志物量的可检测信号。还提供了其他方面。

19. 发明申请

US20090263834A1 Droplet Actuator Devices and Methods for Immunoassays and Washing 有权
标题翻译：滴液致动器装置和免疫测定和洗涤方法
公开(公告)号：US20090263834A1
公开(公告)日：2009-10-22
申请号：US12393534
申请日：2009-02-26
申请人： Ramakrishna Sista , Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan
发明人： Ramakrishna Sista , Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan
IPC分类号： G01N33/542
CPC分类号： G01N33/54333
摘要： Droplet actuator devices and methods for immunoassays and washing are provided. According to one embodiment, a method of providing a droplet in contact with a surface of a super paramagnetic bead with a reduced concentration of a substance is provided and includes: (a) providing a super paramagnetic bead in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets. The set of droplets includes: (i) a droplet in contact with the super paramagnetic bead having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the super paramagnetic bead.
摘要翻译：提供液滴致动器装置和免疫测定和洗涤方法。根据一个实施方案，提供了一种提供与物质浓度降低的超顺磁珠的表面接触的液滴的方法，包括：（a）提供与包含起始浓度的液滴接触的超顺磁珠并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合液滴以产生一组液滴。一组液滴包括：（i）与超顺磁珠接触的液滴，其物质相对于起始浓度降低; 和（ii）从超顺磁珠分离的液滴。

20. 发明授权

US09675972B2 Method of concentrating beads in a droplet 有权
公开(公告)号：US09675972B2
公开(公告)日：2017-06-13
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
IPC分类号： B01L3/00 , B01L3/02 , B01F13/00 , B01F13/08 , B03C1/01 , B03C1/28
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.

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