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    • 11. 发明申请
    • TRANSCRIPTION FACTORS THAT ENHANCE TRAITS IN PLANT ORGANS
    • 在工厂机关加强旅行的转录因素
    • US20100154078A1
    • 2010-06-17
    • US12689010
    • 2010-01-18
    • ANN L. T. POWELLOLIVER J. RATCLIFFET. LYNNE REUBERALAN B. BENNETT
    • ANN L. T. POWELLOLIVER J. RATCLIFFET. LYNNE REUBERALAN B. BENNETT
    • A01H5/00C12N15/82
    • C07K14/415C12N15/8214C12N15/8247C12N15/8261C12N15/8267C12N15/8271C12N15/8273C12N15/8275C12N15/8282Y02A40/146
    • Expression of two Arabidopsis thaliana GARP-family transcription factors, AtGLK1, SEQ ID NO: 2, and AtGLK2, SEQ ID NO: 4, in tomato plants resulted in intensely green fruit that ripen to a normal red color. These Golden2-like (GLK) transcription factors were expressed under the control of several promoters in transgenic tomato lines. When AtGLK1 or AtGLK2 expression was regulated with the constitutive 35S promoter or with three promoters that enhanced expression in fruit tissues, the chlorophyll content of mature green fruit was increased by as much as 100%. The chloroplasts in green fruit expressing AtGLK1 or AtGLK2 developed earlier, were enlarged and had more extensive thylakoid granal development. In addition, expression of AtGLK1 or AtGLK2 resulted in increased starch accumulation in green fruit and higher levels of sugars in ripe fruit. In contrast to wild-type fruit, fruit expressing AtGLK1 developed full green color when they developed in the absence of light. Manipulation of the expression of GLK-like transcription factors in plants may provide a means for improving plant organ nutritional properties, particularly in plants or plant organs grown or maintained under low irradiance.
    • 番茄植物中两种拟南芥GARP家族转录因子AtGLK1,SEQ ID NO:2和AtGLK2,SEQ ID NO:4的表达导致成熟为正常红色的强绿色果实。 这些Golden2样(GLK)转录因子在转基因番茄品系的几种启动子的控制下表达。 当AtGLK1或AtGLK2表达受组成型35S启动子调控时,或3种启动子在果实组织中表达增强时,成熟绿色果实的叶绿素含量增加高达100%。 表达AtGLK1或AtGLK2的绿色果实中的叶绿体早期发育,扩大,并具有更广泛的类囊体发育。 此外,AtGLK1或AtGLK2的表达导致成熟果实中绿色果实中的淀粉积累增加和较高水平的糖。 与野生型水果相比,表达AtGLK1的水果在没有光照时开发出全绿色。 在植物中操纵GLK样转录因子的表达可以提供改善植物器官营养特性的手段,特别是在生长或维持在低辐照度下的植物或植物器官中。