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11. 发明申请

US20090011503A1 Primate Embryonic Stem Cells 有权
标题翻译：灵长类胚胎干细胞
公开(公告)号：US20090011503A1
公开(公告)日：2009-01-08
申请号：US12047135
申请日：2008-03-12
申请人： James A. Thomson
发明人： James A. Thomson
IPC分类号： C12N5/08 , C12N5/02
CPC分类号： C12N5/0605 , C12N5/0603 , C12N5/0606 , C12N2502/13 , C12N2506/02
摘要： A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
摘要翻译：公开了灵长类动物胚胎干细胞的纯化制剂。该制剂的特征在于以下细胞表面标志物：SSEA-1（ - ）; SSEA-4（+）; TRA-1-60（+）; TRA-1-81（+）; 碱性磷酸酶（+）。在一个特别有利的实施方案中，制剂的细胞是人胚胎干细胞，具有正常的核型，并且在连续培养11个月后，以未分化状态继续增殖。胚胎干细胞系还保留了整个培养物中形成滋养细胞并分化成来自所有三个胚胎胚层（内胚层，中胚层和外胚层）的所有组织的能力。还公开了分离灵长类动物胚胎干细胞系的方法。

12. 发明授权

US07449334B2 Medium containing pipecholic acid and gamma amino butyric acid and culture of embryonic stem cells 有权
标题翻译：含有哌醋酸和γ-氨基丁酸的培养基以及胚胎干细胞的培养
公开(公告)号：US07449334B2
公开(公告)日：2008-11-11
申请号：US11221457
申请日：2005-09-08
申请人： James A. Thomson , Tenneille Ludwig
发明人： James A. Thomson , Tenneille Ludwig
IPC分类号： C12N5/08 , C12N5/02
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2500/12 , C12N2500/20 , C12N2500/25 , C12N2500/32 , C12N2500/36 , C12N2500/38 , C12N2500/50 , C12N2500/90 , C12N2500/98 , C12N2501/115 , C12N2501/15 , C12N2501/845
摘要： Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor, gamma amino butyric acid, pipecholic acid, lithium and transforming growth factor beta are added to the medium in which the stem cells are cultured, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium.
摘要翻译：用于培养人胚胎干细胞的以前的方法需要成纤维细胞饲养细胞或已经暴露于成纤维细胞饲养细胞的培养基，以便保持干细胞处于未分化状态。现在已经发现，如果将高水平的成纤维细胞生长因子，γ氨基丁酸，哌醋酸，锂和转化生长因子β加入到其中培养干细胞的培养基中，则干细胞将通过多次而无限期地保持未分化通道，甚至没有饲养细胞或条件培养基。

13. 发明授权

US07220584B2 Method of making embryoid bodies from primate embryonic stem cells 有权
标题翻译：从灵长类动物胚胎干细胞制备胚状体的方法
公开(公告)号：US07220584B2
公开(公告)日：2007-05-22
申请号：US10632399
申请日：2003-08-01
申请人： James A Thomson , Vivienne S. Marshall , Jennifer J. Swiergiel
发明人： James A Thomson , Vivienne S. Marshall , Jennifer J. Swiergiel
IPC分类号： C12N5/02 , C12N5/04 , C12N5/08 , A01N1/02
CPC分类号： C12N5/0603
摘要： Primate embryoid bodies are formed from primate ES cells. The ES cells form clumps. One then removes the clumps, as clumps, and permits incubation under non-adherent conditions. The development of embryoid bodies from primate ES cells is dependent on maintaining the aggregation of cells, as individualized cells will rapidly die.
摘要翻译：灵长类胚体由灵长类ES细胞形成。 ES细胞形成团块。然后一个去除团块，如团块，并允许在非粘附条件下孵育。灵长类动物ES细胞中胚状体的发育依赖于维持细胞的聚集，因为个体细胞会迅速死亡。

14. 发明授权

US07005252B1 Serum free cultivation of primate embryonic stem cells 有权
公开(公告)号：US07005252B1
公开(公告)日：2006-02-28
申请号：US09522030
申请日：2000-03-09
申请人： James A Thomson
发明人： James A Thomson
IPC分类号： A01N1/00 , C12Q1/00 , C12P1/00 , C12N5/00 , C12N5/06
CPC分类号： C12N5/0606 , C12N2500/90 , C12N2501/115
摘要： Disclosed herein are methods for culturing primate embryonic stem cells. These cells are cultured on a prolonged and stable basis in the presence of exogenously supplied fibroblast growth factor and in the absence of animal serum. Preferably there is also a fibroblast feeder layer. Also disclosed is a culture media containing fibroblast feeder layer and the fibroblast growth factor.

15. 发明授权

US06200806B1 Primate embryonic stem cells 失效
公开(公告)号：US06200806B1
公开(公告)日：2001-03-13
申请号：US09106390
申请日：1998-06-26
申请人： James A. Thomson
发明人： James A. Thomson
IPC分类号： C12N508
CPC分类号： C12N5/0605 , C12N5/0603 , C12N5/0606 , C12N2502/13 , C12N2506/02
摘要： A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.

16. 发明授权

US08642337B2 Defined surfaces of self-assembled monolayers and stem cells 有权
标题翻译：自组装单层和干细胞的定义表面
公开(公告)号：US08642337B2
公开(公告)日：2014-02-04
申请号：US13291555
申请日：2011-11-08
申请人： Laura L. Kiessling , Ratmir Derda , Brendan P. Orner , James A. Thomson
发明人： Laura L. Kiessling , Ratmir Derda , Brendan P. Orner , James A. Thomson
IPC分类号： C12N5/00 , C12N5/02 , C12N5/071 , C12N1/00
CPC分类号： C12N5/0068 , C12N2503/02 , C12N2535/10 , G01N33/569 , G01N2610/00
摘要： A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.
摘要翻译：描述了用于从自组装单层构建阵列的方法。阵列对于能够促进培养细胞生长的肽配体的筛选具有特别的用途。已经使用这种技术鉴定促进人类干细胞生长的肽配体，否则需要细胞外基质以在未分化状态下生长。这也使鉴定其他这样的肽成为可能。

17. 发明授权

US08440461B2 Reprogramming somatic cells using retroviral vectors comprising Oct-4 and Sox2 genes 有权
标题翻译：使用包含Oct-4和Sox2基因的逆转录病毒载体重编程体细胞
公开(公告)号：US08440461B2
公开(公告)日：2013-05-14
申请号：US12053440
申请日：2008-03-21
申请人： James A. Thomson , Junying Yu
发明人： James A. Thomson , Junying Yu
IPC分类号： C12N15/00
CPC分类号： C12N5/0607 , C12N5/0606 , C12N5/0696 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/608 , C12N2502/99 , C12N2510/00 , C12N2799/027
摘要： The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.
摘要翻译：本发明涉及通过向体细胞施用至少一种或多种效力决定因素来将体细胞重编程为多能性的方法。本发明还涉及使用重编程方法获得的多能细胞群体。

18. 发明申请

US20120328582A1 Primate Embryonic Stem Cells 审中-公开
标题翻译：灵长类胚胎干细胞
公开(公告)号：US20120328582A1
公开(公告)日：2012-12-27
申请号：US13595587
申请日：2012-08-27
申请人： James A. Thomson
发明人： James A. Thomson
IPC分类号： A61K35/12 , A61P37/00 , A61P3/10 , C12N5/0735 , A61P25/16
CPC分类号： C12N5/0605 , C12N5/0603 , C12N5/0606 , C12N2502/13 , C12N2506/02
摘要： A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
摘要翻译：公开了灵长类动物胚胎干细胞的纯化制剂。该制剂的特征在于以下细胞表面标志物：SSEA-1（ - ）; SSEA-4（+）; TRA-1-60（+）; TRA-1-81（+）; 和碱性磷酸酶（+）。在一个特别有利的实施方案中，制剂的细胞是人胚胎干细胞，具有正常的核型，并且在连续培养11个月后，以未分化状态继续增殖。胚胎干细胞系还保留了整个培养物中形成滋养细胞并分化成来自所有三个胚胎胚层（内胚层，中胚层和外胚层）的所有组织的能力。还公开了分离灵长类动物胚胎干细胞系的方法。

19. 发明申请

US20120178160A1 Cultivation Of Primate Embryonic Stem Cells 审中-公开
标题翻译：灵长类胚胎干细胞的培养
公开(公告)号：US20120178160A1
公开(公告)日：2012-07-12
申请号：US13398933
申请日：2012-02-17
申请人： James A. Thomson , Mark Levenstein , Ren-He Xu
发明人： James A. Thomson , Mark Levenstein , Ren-He Xu
IPC分类号： C12N5/0735
CPC分类号： C12N5/0606 , C12N2500/44 , C12N2501/115 , C12N2501/155
摘要： The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferrin, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than just a feeder layer the medium. Also disclosed are compositions capable of supporting the culture and proliferation of human embryonic stem cells without the need for feeder cells or for exposure of the medium to feeder cells.
摘要翻译：本发明涉及通过在基本上不含哺乳动物胎儿血清的环境中培养干细胞以及在含有氨基酸，维生素，盐，矿物质，转铁蛋白，胰岛素，白蛋白和干扰素的干细胞培养基中培养人胚胎干细胞的方法。成纤维细胞生长因子从源而不仅仅是饲养层提供给培养基。还公开了能够支持人胚胎干细胞的培养和增殖而不需要饲养细胞或将培养基暴露于饲养细胞的组合物。

20. 发明授权

US08012751B2 Differentiation of pluripotent embryonic stem cells 有权
标题翻译：多能胚胎干细胞的分化
公开(公告)号：US08012751B2
公开(公告)日：2011-09-06
申请号：US11395657
申请日：2006-03-31
申请人： James A. Thomson , Thomas P. Zwaka
发明人： James A. Thomson , Thomas P. Zwaka
IPC分类号： C12N5/00
CPC分类号： C12N5/0606 , C12N2501/48 , C12N2501/70
摘要： The invention relates to a method to induce primate embryonic stem cells to differentiate into a relatively homogenous population of mesendoderm cells by treatment with caspase-like inhibitors. Also described is a population of mesendoderm cells obtained therefrom. The embryonic stem cell derived mesendoderm cells have the general morphological and cell surface marker characteristics of mesendoderm cells.
摘要翻译：本发明涉及通过用半胱天冬酶样抑制剂处理来诱导灵长类动物胚胎干细胞分化为相对均匀的中内胚层细胞群的方法。还描述了从其获得的中胚层细胞群。胚胎干细胞衍生的中胚层细胞具有中内根异质细胞的一般形态和细胞表面标志物特征。

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