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11. 发明申请

US20070117220A1 METHOD AND APPARATUS FOR MEASURING FLUORESCENCE POLARIZATION IN LAB-ON-A-CHIP 失效
标题翻译：用于测量片上荧光偏振的方法和装置
公开(公告)号：US20070117220A1
公开(公告)日：2007-05-24
申请号：US11422142
申请日：2006-06-05
申请人： Eun Gyeong YANG , Jung Hwan Kim , Tae Kim , Byeong-Kwon Ju , Ji Kang , Hyun Shin , Han-Sang Cho
发明人： Eun Gyeong YANG , Jung Hwan Kim , Tae Kim , Byeong-Kwon Ju , Ji Kang , Hyun Shin , Han-Sang Cho
IPC分类号： G01N33/543 , C12M3/00
CPC分类号： G01N33/542 , B01L3/5027 , G01N21/05 , G01N21/6408 , G01N21/6428 , G01N21/6445 , G01N2021/0346
摘要： Disclosed relates to a method and an apparatus for measuring fluorescence polarization FP in a lab-on-a-chip and, more concretely, to a method and an apparatus that measure quantitatively interactions between biomolecules and fluorescently labeled biomaterials and enzyme activities using the measurement of fluorescence polarization FP. The method and the apparatus of the invention provide rapid assays with minute amounts of samples using an automated device compared to conventional methods. Accordingly, the method and the apparatus of the invention can be usefully applied to the measurement of interactions between biomolecules and to the protease assays using a protein substrate.
摘要翻译：本发明涉及一种用于在芯片实验室中测量荧光偏振FP的方法和装置，更具体地说，涉及一种使用测量的方法和装置来量测生物分子与荧光标记的生物材料和酶活性之间的相互作用荧光偏振FP。与常规方法相比，本发明的方法和设备使用自动化装置提供使用微量样品的快速测定。因此，本发明的方法和装置可以有效地应用于使用蛋白质底物的生物分子与蛋白酶测定之间的相互作用的测量。

12. 发明申请

US20070020718A1 METHOD FOR QUANTITATIVE ANALYSIS OF PROLINE HYDROXYLATION-INDUCED INTERACTIONS BETWEEN HIF-1 AND VBC PROTEIN COMPLEX USING FLUORESCENCE POLARIZATION 失效
标题翻译：使用荧光偏振法定量分析HIF-1和VBC蛋白复合物之间的羟基羟化诱导相互作用的方法
公开(公告)号：US20070020718A1
公开(公告)日：2007-01-25
申请号：US11164813
申请日：2005-12-06
申请人： Eun Gyeong YANG , Hyun Ju CHO , Hyunsung PARK
发明人： Eun Gyeong YANG , Hyun Ju CHO , Hyunsung PARK
IPC分类号： C12Q1/30
CPC分类号： G01N33/6875 , G01N33/6872 , G01N2500/00
摘要： The present invention relates to a method for analyzing the interaction between HIF-1 peptide and VBC protein using fluorescence polarization, more precisely, a method for quantitative analysis of formation of HIF-1-VBC protein complex which is composed of the steps of 1) preparing a fluorescent probe by attaching a fluorescein to hydroxyproline containing HIF-1 peptide; 2) reacting the fluorescent probe with VBC protein; and 3) measuring the fluorescence polarization of the above reactant and then comparing the fluorescence polarization with that of the fluorescent probe itself to investigate the changes of fluorescence polarization; a method for screening an inhibitor of the binding of HIF-1 peptide and VBC protein using the above method; and a method for analyzing the activity of prolyl hydroxylase using the above method. The method of the present invention enables simple analysis of the interaction between HIF-1 peptide and VBC protein by observing changes of fluorescence polarization and thus, it can be effectively used for high-speed screening using a well plate.
摘要翻译：本发明涉及使用荧光偏振分析HIF-1肽与VBC蛋白之间相互作用的方法，更确切地说，涉及HIF-1-VBC蛋白复合物形成定量分析方法，该方法由以下步骤组成：1）通过将荧光素附着到含有HIF-1肽的羟脯氨酸上制备荧光探针; 2）使荧光探针与VBC蛋白反应; 和3）测量上述反应物的荧光偏振，然后将荧光偏振与荧光探针本身的荧光偏振进行比较，以研究荧光偏振的变化; 使用上述方法筛选HIF-1肽和VBC蛋白的结合抑制剂的方法; 以及使用上述方法分析脯氨酰羟化酶的活性的方法。通过观察荧光偏振的变化，本发明的方法能够简单地分析HIF-1肽与VBC蛋白之间的相互作用，因此可以有效地用于使用孔板的高速筛选。

13. 发明授权

US07691966B2 Method for quantitative analysis for prolyl hydroxylase activity using fluorescence polarization 失效
标题翻译：使用荧光偏振定量分析脯氨酰羟化酶活性的方法
公开(公告)号：US07691966B2
公开(公告)日：2010-04-06
申请号：US11164813
申请日：2005-12-06
申请人： Eun Gyeong Yang , Hyun Ju Cho , Hyunsung Park
发明人： Eun Gyeong Yang , Hyun Ju Cho , Hyunsung Park
IPC分类号： C07K14/00 , C12Q1/00 , C12Q1/30
CPC分类号： G01N33/6875 , G01N33/6872 , G01N2500/00
摘要： The present invention relates to a method for analyzing the interaction between HIF-1 peptide and VBC protein using fluorescence polarization, more precisely, a method for quantitative analysis of formation of HIF-1-VBC protein complex which is composed of the steps of 1) preparing a fluorescent probe by attaching a fluorescein to hydroxyproline containing HIF-1 peptide; 2) reacting the fluorescent probe with VBC protein; and 3) measuring the fluorescence polarization of the above reactant and then comparing the fluorescence polarization with that of the fluorescent probe itself to investigate the changes of fluorescence polarization; a method for screening an inhibitor of the binding of HIF-1 peptide and VBC protein using the above method; and a method for analyzing the activity of prolyl hydroxylase using the above method. The method of the present invention enables simple analysis of the interaction between HIF-1 peptide and VBC protein by observing changes of fluorescence polarization and thus, it can be effectively used for high-speed screening using a well plate.
摘要翻译：本发明涉及使用荧光偏振分析HIF-1肽与VBC蛋白之间相互作用的方法，更确切地说，涉及HIF-1-VBC蛋白复合物形成定量分析方法，该方法由以下步骤组成：1）通过将荧光素附着到含有HIF-1肽的羟脯氨酸上制备荧光探针; 2）使荧光探针与VBC蛋白反应; 和3）测量上述反应物的荧光偏振，然后将荧光偏振与荧光探针本身的荧光偏振进行比较，以研究荧光偏振的变化; 使用上述方法筛选HIF-1肽和VBC蛋白的结合抑制剂的方法; 以及使用上述方法分析脯氨酰羟化酶的活性的方法。通过观察荧光偏振的变化，本发明的方法能够简单地分析HIF-1肽与VBC蛋白之间的相互作用，因此可以有效地用于使用孔板的高速筛选。

14. 发明申请

US20080124790A1 BIOSENSOR FOR THE DETECTION OF PROTEIN KINASE A, AND KIT COMPRISING THE SAME 有权
标题翻译：用于检测蛋白激酶A的生物传感器和包含其的试剂盒
公开(公告)号：US20080124790A1
公开(公告)日：2008-05-29
申请号：US11551614
申请日：2006-10-20
申请人： Eun-Gyeong YANG , Ki-Cheol HAN , Dae-Sung YOON , Tae-Song KIM
发明人： Eun-Gyeong YANG , Ki-Cheol HAN , Dae-Sung YOON , Tae-Song KIM
IPC分类号： C12M1/14
CPC分类号： C12Q1/485 , C12Q1/001 , G01N33/54373
摘要： The present invention relates to a biosensor specifically detecting a protein kinase A (PKA), and a diagnosis kit comprising the biosensor. In particular, the present invention relates to a biosensor for detecting a PKA comprising a cantilever, and a protein kinase inhibitor (PKI) immobilized on the surface of the cantilever; and a kit for diagnosing diseases caused by the increase of the activity of a protein kinase, comprising a sample, the biosensor, and a quantification manner.
摘要翻译：本发明涉及一种特异性检测蛋白激酶A（PKA）的生物传感器和包含生物传感器的诊断试剂盒。特别地，本发明涉及用于检测包含悬臂的PKA和固定在悬臂表面上的蛋白激酶抑制剂（PKI）的生物传感器; 以及用于诊断由蛋白激酶的活性增加引起的疾病的试剂盒，其包含样品，生物传感器和定量方式。

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