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11. 发明申请

US20100159516A1 ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION 有权
标题翻译：生物质有机溶剂预处理提高酶的浓度
公开(公告)号：US20100159516A1
公开(公告)日：2010-06-24
申请号：US12639033
申请日：2009-12-16
申请人： Bruce A. Diner , Janine Fan
发明人： Bruce A. Diner , Janine Fan
IPC分类号： C12P7/02 , C07H1/08 , C12P19/00 , C12P7/40
CPC分类号： D21C3/003 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , C13K1/02 , D21C3/006 , D21C3/20 , Y02E50/16 , Y02E50/17
摘要： Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of one or more alkylamine and optionally one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.
摘要翻译：在碱性条件下，在一种或多种烷基胺和任选的一种或多种另外的亲核试剂存在下，使用有机溶剂溶液对生物质进行预处理以分离和提取木质素。预处理的生物质被糖化酶联合体进一步水解。通过糖化释放的发酵糖可用于通过发酵生产目标化学品。

12. 发明授权

US07588941B2 Dispersion of carbon nanotubes by nucleic acids 失效
标题翻译：通过核酸分散碳纳米管
公开(公告)号：US07588941B2
公开(公告)日：2009-09-15
申请号：US11590564
申请日：2006-10-31
申请人： Ming Zheng , Bruce A. Diner
发明人： Ming Zheng , Bruce A. Diner
IPC分类号： C12Q1/68 , C12M1/00 , C07H21/04
CPC分类号： C07H21/04 , B82Y5/00 , B82Y10/00 , B82Y30/00
摘要： Nucleic acid molecules in a stabilized solution such as single stranded DNA and RNA were able to disperse high concentration of bundled carbon nanotubes into aqueous solution.
摘要翻译：稳定溶液如单链DNA和RNA中的核酸分子能够将高浓度的束状碳纳米管分散到水溶液中。

13. 发明授权

US10138499B2 Methods for improving the efficiency of simultaneous saccharification and fermentation reactions 有权
公开(公告)号：US10138499B2
公开(公告)日：2018-11-27
申请号：US13516610
申请日：2010-12-17
申请人： Christina Gutierrez , Colin Mitchinson , Tom T. Huang , Bruce A. Diner , Paul Joseph Fagan , William D. Hitz
发明人： Christina Gutierrez , Colin Mitchinson , Tom T. Huang , Bruce A. Diner , Paul Joseph Fagan , William D. Hitz
IPC分类号： C12P7/06 , C12N1/22 , C12N9/42 , C12N9/24 , C12P7/04 , C12P7/10 , C12P7/16 , C12P19/44 , C12N15/80 , C12P7/18 , C12P19/02
摘要： The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.

14. 发明申请

US20130143277A1 Methods for Improving the Efficiency of Simultaneous Saccharification and Fermentation Reactions 审中-公开
标题翻译：提高同时糖化和发酵反应效率的方法
公开(公告)号：US20130143277A1
公开(公告)日：2013-06-06
申请号：US13516610
申请日：2010-12-17
申请人： Christina Gutierrez , Colin Mitchinson , Tom T. Huang , Bruce A. Diner , Paul Joseph Fagan , William D. Hitz
发明人： Christina Gutierrez , Colin Mitchinson , Tom T. Huang , Bruce A. Diner , Paul Joseph Fagan , William D. Hitz
IPC分类号： C12P7/06 , C12P19/02 , C12P7/18 , C12N15/80 , C12P7/04 , C12P7/16
CPC分类号： C12P7/06 , C12N1/22 , C12N9/2434 , C12N9/2477 , C12N9/248 , C12N15/80 , C12P7/04 , C12P7/10 , C12P7/16 , C12P7/18 , C12P19/02 , C12P19/44 , C12Y302/01037 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.
摘要翻译：本公开的第一方面涉及使用β-木糖苷酶逆转以减少副产物形成并增加发酵产物的产量，以及在第二方面，使用保留β-木糖苷酶在同时糖化和发酵反应中改善烷基-β-木吡喃糖苷化合物的生产。

15. 发明申请

US20120208246A1 PRODUCTION OF ALCOHOL ESTERS AND IN SITU PRODUCT REMOVAL DURING ALCOHOL FERMENTATION 有权
标题翻译：在酒精发酵期间生产酒精产品和脱除产品
公开(公告)号：US20120208246A1
公开(公告)日：2012-08-16
申请号：US13193147
申请日：2011-07-28
申请人： Douglas Robert Anton , Stephane Francois Bazzana , Keith H. Burlew , Jelena Cirakovic , Robert Dicosimo , Bruce A. Diner , Michael Charles Grady , Brian Michael Roesch , Francis J. Woerner
发明人： Douglas Robert Anton , Stephane Francois Bazzana , Keith H. Burlew , Jelena Cirakovic , Robert Dicosimo , Bruce A. Diner , Michael Charles Grady , Brian Michael Roesch , Francis J. Woerner
IPC分类号： C12P7/64 , C12N9/16 , A23K1/06 , C12P7/16
CPC分类号： A23K1/007 , A23K10/12 , A23K10/38 , C12P7/16 , C12P7/62 , C12P7/649 , C12P2203/00 , Y02E50/10 , Y02E50/13 , Y02P60/873
摘要： An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium.
摘要翻译：醇发酵方法和组合物，其包括通过在发酵培养基中与产物醇与羧酸（例如，脂肪酸）和能够酯化产物醇（例如丁醇）的催化剂（例如脂肪酶）酯化产生醇酯，与羧酸形成醇酯。醇酯可以从发酵培养基中提取，并从醇酯中回收产物醇。羧酸也可以用作从发酵培养基中除去醇酯的提取剂。

16. 发明申请

US20100159521A1 OZONE TREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION 审中-公开
标题翻译：生物质的臭氧处理以增强酶的浓度
公开(公告)号：US20100159521A1
公开(公告)日：2010-06-24
申请号：US12640088
申请日：2009-12-17
申请人： Jelena Cirakovic , Bruce A. Diner
发明人： Jelena Cirakovic , Bruce A. Diner
IPC分类号： C12P19/00 , C07H1/08
CPC分类号： D21C3/024 , C08H8/00 , C10G2300/1011 , C10L1/02 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , C13K1/02 , D21C3/028 , D21C3/20 , Y02E50/16 , Y02P30/20
摘要： Methods for treating lignocellulosic biomass to produce readily saccharifiable carbohydrate-enriched biomass are provided. In one method, lignocellulosic biomass comprising lignin is treated with aqueous ammonia, then contacted with a gas comprising ozone at a temperature of about 0° C. to about 50° C. In another method, lignocellulosic biomass comprising lignin is contacted with a gas comprising ozone at a temperature of about 0° C. to about 50° C., then treated with aqueous ammonia. The readily saccharifiable carbohydrate-enriched biomass may be saccharified with an enzyme consortium to produce fermentable sugars.
摘要翻译：提供了用于处理木质纤维素生物质以生产易于糖化的富含碳水化合物的生物质的方法。在一种方法中，包含木质素的木质纤维素生物质用氨水处理，然后在约0℃至约50℃的温度下与包含臭氧的气体接触。在另一种方法中，包含木质素的木质纤维素生物质与包含臭氧在约0℃至约50℃的温度下，然后用氨水处理。容易糖化的富含碳水化合物的生物质可以用酶联合糖化糖化以产生可发酵的糖。

17. 发明申请

US20100159518A1 ORGANIC SOLVENT PRETREATMENT OF BIOMASS TO ENHANCE ENZYMATIC SACCHARIFICATION 有权
标题翻译：生物质有机溶剂预处理提高酶的浓度
公开(公告)号：US20100159518A1
公开(公告)日：2010-06-24
申请号：US12639045
申请日：2009-12-16
申请人： Bruce A. Diner , Janine Fan
发明人： Bruce A. Diner , Janine Fan
IPC分类号： C12P7/02 , C07H1/08 , C12P19/00 , C12P7/40
CPC分类号： C13K1/02 , C08H6/00 , C08H8/00 , C12P2201/00 , D21C11/0007 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of one of more sulfide (hydrosulfide) salt and optionally one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.
摘要翻译：在碱性条件下，在一种更多的硫化物（氢硫化物）盐和任选的一种或多种另外的亲核试剂存在下，使用有机溶剂溶液预处理生物质，以将木质素分解和提取。预处理的生物质被糖化酶联合体进一步水解。通过糖化释放的发酵糖可用于通过发酵生产目标化学品。

18. 发明授权

US07635423B2 Redox potential mediated, heterogeneous, carbon nanotube biosensing 失效
标题翻译：氧化还原电位介导的，异质的碳纳米管生物传感
公开(公告)号：US07635423B2
公开(公告)日：2009-12-22
申请号：US11241515
申请日：2005-09-30
申请人： Salah Boussaad , Bruce A. Diner , Janine Fan , Vsevolod Rostovtsev , Ajit Krishnan
发明人： Salah Boussaad , Bruce A. Diner , Janine Fan , Vsevolod Rostovtsev , Ajit Krishnan
IPC分类号： G01N33/50
CPC分类号： G01N27/127 , B82Y15/00 , B82Y30/00 , Y10S977/92 , Y10S977/957
摘要： This invention relates to the field of nanotechnology. Specifically the invention describes a nanosensor for the detection of an analyte in which the redox potential in solution is altered thereby causing changes in carbon nanotube conductance.
摘要翻译：本发明涉及纳米技术领域。具体地说，本发明描述了一种用于检测其中溶液中的氧化还原电位被改变从而导致碳纳米管传导变化的分析物的纳米传感器。

19. 发明授权

US09249076B2 Recyclable extractant compositions 有权
标题翻译：可循环萃取剂组合物
公开(公告)号：US09249076B2
公开(公告)日：2016-02-02
申请号：US14032328
申请日：2013-09-20
申请人： Douglas Robert Anton , Bruce A. Diner , Steven D. Doig
发明人： Douglas Robert Anton , Bruce A. Diner , Steven D. Doig
IPC分类号： C12P21/06 , C12P7/04 , C07C29/86 , C12P7/16
CPC分类号： C07C29/86 , C12P7/04 , C12P7/16 , C12P21/06 , Y02E50/10
摘要： Provided herein are processes comprising providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol; contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; recovering at least a portion of the butanol and extractant composition from the fermentation medium; recycling the extractant composition recovered in (c) one or more times in the fermentation medium; optionally adjusting the effective amount of antioxidant or antioxidant-like compound in the extractant composition, whereby the rate of oxidation or effect of oxidized products in the recycled extractant composition is substantially reduced and/or avoided such that the extractant composition may be recycled.
摘要翻译：本文提供的方法包括提供包含可发酵碳源的发酵培养基，包含工程丁醇生物合成途径的重组微生物和丁醇; 使所述发酵培养基与包含有效量的抗氧化剂或抗氧化剂样化合物的提取剂组合物接触，由此发酵培养基中的至少一部分丁醇分配到提取剂中; 从发酵培养基中回收至少一部分丁醇和提取剂组合物; 在（c）中回收的萃取剂组合物在发酵培养基中回收一次或多次; 任选地调节萃取剂组合物中抗氧化剂或抗氧化剂样化合物的有效量，从而大大降低和/或避免了再循环的萃取剂组合物中的氧化产物的氧化速率或氧化产物的影响，从而萃取剂组合物可循环使用。

20. 发明授权

US09175315B2 Production of alcohol esters and in situ product removal during alcohol fermentation 有权
公开(公告)号：US09175315B2
公开(公告)日：2015-11-03
申请号：US13162868
申请日：2011-06-17
申请人： Douglas Robert Anton , Stephane Francois Bazzana , Keith H Burlew , Jelena Cirakovic , Robert Dicosimo , Bruce A Diner , Michael Charles Grady , Brian Michael Roesch , Francis J Woerner
发明人： Douglas Robert Anton , Stephane Francois Bazzana , Keith H Burlew , Jelena Cirakovic , Robert Dicosimo , Bruce A Diner , Michael Charles Grady , Brian Michael Roesch , Francis J Woerner
IPC分类号： C12P7/62 , C12P7/22 , C12P7/16 , C12N1/00 , C12N1/12 , C07H21/04 , B01D3/00 , B01D21/26 , C07C29/86 , C11B1/02 , C11B13/00 , C12M1/00 , C12P7/64 , C12P7/18
CPC分类号： C12P7/16 , A23K10/38 , B01D3/002 , B01D11/0492 , B01D21/26 , B01D21/262 , C07C29/86 , C11B1/025 , C11B13/00 , C12F3/10 , C12M1/00 , C12M21/12 , C12M45/04 , C12M45/09 , C12N1/14 , C12N15/00 , C12P7/18 , C12P7/6418 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02W30/74
摘要： An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium.

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