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161. 发明申请

US20130164835A1 SYSTEMS AND METHODS FOR CONTAMINANT REMOVAL FROM A MICROALGAE CULTURE 审中-公开
标题翻译：从微藻文化中去除污染物的系统和方法
公开(公告)号：US20130164835A1
公开(公告)日：2013-06-27
申请号：US13534924
申请日：2012-06-27
申请人： JASON LICAMELE , Anna Lee Y. Tonkovich , Alexandar Sitek , Scott Kuhlman
发明人： JASON LICAMELE , Anna Lee Y. Tonkovich , Alexandar Sitek , Scott Kuhlman
IPC分类号： C12M1/04 , C12M1/12
CPC分类号： C12M1/04 , C12M37/00 , C12M43/00 , C12M47/02
摘要： Systems and methods for the removal of contaminants from a liquid culture microalgac and/or cyanobacteria comprise an inlet tube, a pump, a gas injector, a vertical chamber, and/or a collection container that promotes the production of foam in the microalgae culture, wherein the foam contains the contaminants.
摘要翻译：用于从液体培养微藻和/或蓝细菌中除去污染物的系统和方法包括促进微藻培养物中的泡沫的生产的入口管，泵，气体注射器，垂直室和/或收集容器，其中泡沫包含污染物。

162. 发明申请

US20130072661A1 EXTRACTION OF PROTEINS BY A TWO SOLVENT METHOD 失效
标题翻译：通过两种溶剂法提取蛋白质
公开(公告)号：US20130072661A1
公开(公告)日：2013-03-21
申请号：US13674631
申请日：2012-11-12
申请人： HELIAE DEVELOPMENT, LLC
发明人： Aniket KALE
IPC分类号： C07K1/14 , C11B1/10
CPC分类号： C11B1/10 , C11B1/108 , C11C3/003 , C12N1/06
摘要： A method for separating proteins from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting proteins from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal proteins from a wet algal biomass. These proteins are high value products which can be used as renewable sources of food and food additives. Neutral lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
摘要翻译：使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整藻细胞中分离蛋白质的方法。一些实施方案包括将完整的藻细胞脱水，然后从藻细胞中提取蛋白质。该方法提供了单步和多步提取方法，其允许藻类蛋白质与湿藻类生物质的有效分离。这些蛋白质是可以用作食品和食品添加剂的可再生资源的高价值产品。提取蛋白质后残留在藻类生物质中的中性脂质可用于产生可再生燃料。

163. 发明申请

US20120283496A1 METHODS OF EXTRACTING NEUTRAL LIPIDS AND PRODUCING BIOFUELS 失效
标题翻译：提取中性脂肪和生产生物技术的方法
公开(公告)号：US20120283496A1
公开(公告)日：2012-11-08
申请号：US13473819
申请日：2012-05-17
申请人： Aniket Kale
发明人： Aniket Kale
IPC分类号： C07C1/20
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： Methods for selective extraction and fractionation of algal lipids and algal products are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which enable efficient separation of algal components. Among these components are neutral lipids synthesized by algae, which are extracted by the methods disclosed herein for the production of renewable fuels.
摘要翻译：公开了藻类脂质和藻类产品的选择性提取和分馏方法。从藻类生物质中选择性地去除产物的方法提供了能够有效分离藻类成分的单步骤和多步提取方法。这些组分中的中性脂质是通过本文公开的用于生产可再生燃料的方法提取的由藻类合成的中性脂质。

164. 发明申请

US20120271038A1 EXTRACTION OF PROTEINS BY A TWO SOLVENT METHOD 失效
标题翻译：通过两种溶剂法提取蛋白质
公开(公告)号：US20120271038A1
公开(公告)日：2012-10-25
申请号：US13540205
申请日：2012-07-02
申请人： Aniket KALE
发明人： Aniket KALE
IPC分类号： C07K14/405
CPC分类号： C11B1/10 , C11B1/108 , C11C3/003 , C12N1/06
摘要： A method for separating proteins from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting proteins from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal proteins from a wet algal biomass. These proteins are high value products which can be used as renewable sources of food and food additives. Neutral lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
摘要翻译：使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整藻细胞中分离蛋白质的方法。一些实施方案包括将完整的藻细胞脱水，然后从藻细胞中提取蛋白质。该方法提供了单步和多步提取方法，其允许藻类蛋白质与湿藻类生物质的有效分离。这些蛋白质是可以用作食品和食品添加剂的可再生资源的高价值产品。提取蛋白质后残留在藻类生物质中的中性脂质可用于产生可再生燃料。

165. 发明授权

US08211309B2 Extraction of proteins by a two solvent method 有权
公开(公告)号：US08211309B2
公开(公告)日：2012-07-03
申请号：US13286904
申请日：2011-11-01
申请人： Aniket Kale
发明人： Aniket Kale
IPC分类号： B01D11/00
CPC分类号： C11B1/10 , C07K1/145 , C11B1/108 , C11C3/003 , C12M21/02 , C12M47/10 , C12N1/06
摘要： A method for separating proteins from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting proteins from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal proteins from a wet algal biomass. These proteins are high value products which can be used as renewable sources of food and food additives. Neutral lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

166. 发明申请

US20120108793A1 Methods of Obtaining Freshwater or Saltwater Algae Products Enriched in Prolamin Proteins 审中-公开
标题翻译：获取富含蛋白质的淡水或盐水藻类产品的方法
公开(公告)号：US20120108793A1
公开(公告)日：2012-05-03
申请号：US13331756
申请日：2011-12-20
申请人： Aniket Kale
发明人： Aniket Kale
IPC分类号： C07K1/14
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
摘要翻译：公开了从藻类生物质或藻类培养物中选择性提取和分离藻类蛋白的方法。从藻类生物质中选择性地除去产物的方法提供允许有效分离藻类蛋白质的单步骤和多步萃取方法。这些蛋白质可用作动物原料和人类食物的可再生来源蛋白质。此外，在提取蛋白质之后残留在藻类生物质中的脂质可用于产生可再生燃料。

167. 发明授权

US08153137B2 Methods of and systems for isolating carotenoids and omega-3 rich oil products from algae 有权
标题翻译：从藻类中分离类胡萝卜素和富含ω-3的油产品的方法和系统
公开(公告)号：US08153137B2
公开(公告)日：2012-04-10
申请号：US13268064
申请日：2011-10-07
申请人： Aniket Kale
发明人： Aniket Kale
IPC分类号： A61K36/00 , A61K36/02
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： A method of isolating nutraceuticals products from algae is provided. A method of isolating carotenoids and omega-3 rich oil from algae includes dewatering substantially intact algal cells to make an algal biomass and adding a first ethanol fraction to the algal biomass. The method also includes separating a first substantially solid biomass fraction from a first substantially liquid fraction comprising proteins and combining the first substantially solid biomass fraction with a second ethanol fraction. The method further includes separating a second substantially solid biomass fraction from a second substantially liquid fraction comprising polar lipids and combining the second substantially solid biomass fraction with a third ethanol solvent fraction. The method also includes separating a third substantially solid biomass fraction from a third substantially liquid fraction comprising neutral lipids, wherein the third substantially solid biomass fraction comprises carbohydrates and separating the neutral lipids into carotenoids and omega-3 rich oil.
摘要翻译：提供了从藻类中分离营养品产品的方法。从藻类中分离类胡萝卜素和富含ω-3的油的方法包括将基本上完整的藻类细胞脱水以制备藻类生物质并向藻类生物质中加入第一乙醇级分。该方法还包括从包含蛋白质的第一基本上液体的馏分中分离第一基本上固体的生物质馏分并将第一基本上固体的生物质馏分与第二乙醇馏分组合。该方法还包括从包含极性脂质的第二基本上液体的部分中分离第二基本上固体的生物质馏分，并将第二基本上固体的生物质馏分与第三乙醇溶剂馏分组合。该方法还包括从包含中性脂质的第三基本上液体的级分分离第三基本上固体的生物质馏分，其中第三基本上固体的生物质级分包含碳水化合物并将中性脂质分离成类胡萝卜素和富含ω3的油。

168. 发明授权

US08142659B2 Extraction with fractionation of oil and proteinaceous material from oleaginous material 有权
$Extraction with fractionation of oil and proteinaceous material from oleaginous material$
标题翻译：从含油材料中分离油和蛋白质材料进行提取
公开(公告)号：US08142659B2
公开(公告)日：2012-03-27
申请号：US13081197
申请日：2011-04-06
申请人： Aniket Kale
发明人： Aniket Kale
IPC分类号： B01D11/00
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： Methods for selective extraction and fractionation of algal lipids and algal products are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which enable efficient separation of algal components. Among these components are neutral lipids synthesized by algae, which are extracted by the methods disclosed herein for the production of renewable fuels.
摘要翻译：公开了藻类脂质和藻类产品的选择性提取和分馏方法。从藻类生物质中选择性地去除产物的方法提供了能够有效分离藻类成分的单步骤和多步提取方法。这些组分中的中性脂质是通过本文公开的用于生产可再生燃料的方法提取的由藻类合成的中性脂质。

169. 发明申请

US20120065378A1 SELECTIVE EXTRACTION OF PROLAMIN PROTEINS FROM FRESHWATER OR SALTWATER ALGAE 审中-公开
标题翻译：从淡水或盐水中选择性提取丙酰胺蛋白
公开(公告)号：US20120065378A1
公开(公告)日：2012-03-15
申请号：US13299893
申请日：2011-11-18
申请人： Aniket Kale
发明人： Aniket Kale
IPC分类号： C07K14/405
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
摘要翻译：公开了从藻类生物质或藻类培养物中选择性提取和分离藻类蛋白的方法。从藻类生物质中选择性地除去产物的方法提供允许有效分离藻类蛋白质的单步骤和多步萃取方法。这些蛋白质可用作动物原料和人类食物的可再生来源蛋白质。此外，在提取蛋白质之后残留在藻类生物质中的脂质可用于产生可再生燃料。

170. 发明申请

US20120053357A1 EXTRACTION OF POLAR LIPIDS BY A TWO SOLVENT METHOD 有权
标题翻译：通过两种溶剂方法提取极性脂肪
公开(公告)号：US20120053357A1
公开(公告)日：2012-03-01
申请号：US13286889
申请日：2011-11-01
申请人： Aniket Kale
发明人： Aniket Kale
IPC分类号： C11B1/10
CPC分类号： C11B1/10 , C11B1/108 , C12P7/6463 , C12P7/649 , Y02E50/13
摘要： A method for separating polar lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting polar lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal polar lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. These polar lipids are high value products which can be used as surfactants, detergents, and food additives. Neutral lipids remaining in the algal biomass after extraction of polar lipids can be used to generate renewable fuels.
摘要翻译：使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整的藻类细胞中分离极性脂质的方法。一些实施方案包括将完整的藻细胞脱水，然后从藻类细胞中提取极性脂质。该方法提供单步和多步提取方法，其允许藻类极性脂质与湿藻类生物质的有效分离，同时避免提取混合物的乳化。这些极性脂质是可用作表面活性剂，洗涤剂和食品添加剂的高价值产品。提取极性脂质后残留在藻类生物质中的中性脂质可用于产生可再生燃料。

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