专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

141. 发明申请

US20150193928A1 HIGH-THROUGHPUT SINGLE-CELL IMAGING, SORTING, AND ISOLATION 审中-公开
标题翻译：高通量单细胞成像，分选和分离
公开(公告)号：US20150193928A1
公开(公告)日：2015-07-09
申请号：US14595693
申请日：2015-01-13
申请人： Rajiv Bharadwaj , Bahram Fathollahi
发明人： Rajiv Bharadwaj , Bahram Fathollahi
IPC分类号： G06T7/00 , H04N7/18
CPC分类号： H04N5/2251 , B01L3/502761 , B01L2200/0636 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , C12M47/04 , C12N5/0081 , C12N5/0087 , G02B21/365 , G06T7/0012 , H04N5/2256 , H04N7/18
摘要： The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.
摘要翻译：本发明提供了一种隔离单个靶细胞的方法和装置。该装置包括主体结构，其包括主通道，收集通道和在第一流体结处流体耦合的废物通道。多个捕获通道与收集通道相交，每个捕获通道在与捕集通道与收集通道的交叉点相邻的位置处的直径小于单个靶细胞的直径。该装置还包括：成像系统，被配置为对主通道内的各个目标单元和非目标单元进行成像，从而产生成像数据; 处理器，被配置为执行所述成像数据的实时，多变量分析; 以及被配置为引导各个目标细胞的引导系统。压力源与收集通道，废物通道，第一侧通道和第二侧通道中的一个或多个流体连通。

142. 发明申请

US20140247971A1 HIGH-THROUGHPUT SINGLE-CELL IMAGING, SORTING, AND ISOLATION 有权
标题翻译：高通量单细胞成像，分选和分离
公开(公告)号：US20140247971A1
公开(公告)日：2014-09-04
申请号：US13783767
申请日：2013-03-04
申请人： Rajiv Bharadwaj , Bahram Fathollahi
发明人： Rajiv Bharadwaj , Bahram Fathollahi
IPC分类号： G06T7/00
CPC分类号： H04N5/2251 , B01L3/502761 , B01L2200/0636 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , C12M47/04 , C12N5/0081 , C12N5/0087 , G02B21/365 , G06T7/0012 , H04N5/2256 , H04N7/18
摘要： The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.
摘要翻译：本发明提供了一种隔离单个靶细胞的方法和装置。该装置包括主体结构，其包括主通道，收集通道和在第一流体结处流体耦合的废物通道。多个捕获通道与收集通道相交，每个捕获通道在与捕集通道与收集通道的交叉点相邻的位置处的直径小于单个靶细胞的直径。该装置还包括：成像系统，被配置为对主通道内的各个目标单元和非目标单元进行成像，从而产生成像数据; 处理器，被配置为执行所述成像数据的实时，多变量分析; 以及被配置为引导各个目标细胞的引导系统。压力源与收集通道，废物通道，第一侧通道和第二侧通道中的一个或多个流体连通。

143. 发明授权

US08414754B1 Electrophoretic sample analysis and approach therefor 有权
标题翻译：电泳样品分析及方法
公开(公告)号：US08414754B1
公开(公告)日：2013-04-09
申请号：US11755449
申请日：2007-05-30
申请人： Juan G. Santiago , Byoungsok Jung , Rajiv Bharadwaj , Tarun Kumar Khurana
发明人： Juan G. Santiago , Byoungsok Jung , Rajiv Bharadwaj , Tarun Kumar Khurana
IPC分类号： B01D57/02 , G01N27/447 , G01N27/453
CPC分类号： G01N27/44769
摘要： Analysis of samples is facilitated. According to an example embodiment, an electrophoresis approach involves electrophoretically stacking and/or separating a sample or samples. An electrolyte and a mixture of one or more samples with another electrolyte are added to a microchannel or capillary. An electric field is applied to stack (and, in some applications, further separate) the one or more samples. Generally, the electric field and electrolyte are used to facilitate isotachophoretic (ITP) stacking. In some embodiments, a further electric field is applied and used with the electrolyte to facilitate subsequent capillary electrophoresis (CE).
摘要翻译：样品分析方便。根据示例性实施方案，电泳方法涉及电泳堆叠和/或分离样品或样品。将电解质和一种或多种样品与另一种电解质的混合物加入到微通道或毛细管中。将一个或多个样品施加到堆叠（并且在一些应用中进一步分离）的电场。通常，电场和电解质用于促进异羟基磷灰石（ITP）堆叠。在一些实施方案中，施加另外的电场并与电解质一起使用以促进随后的毛细管电泳（CE）。

144. 发明授权

US10131903B2 Microfluidic platform for synthetic biology applications 有权
公开(公告)号：US10131903B2
公开(公告)日：2018-11-20
申请号：US13437727
申请日：2012-04-02
申请人： Chieh Chang , Rajiv Bharadwaj , Anup K. Singh , Aarthi Chandrasekaran , Nathan J. Hillson
发明人： Chieh Chang , Rajiv Bharadwaj , Anup K. Singh , Aarthi Chandrasekaran , Nathan J. Hillson
IPC分类号： C12P19/34 , C12N15/10 , C12N15/66 , C12N15/00 , C12P21/06
摘要： This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.

145. 发明授权

US09165359B2 High-throughput single-cell imaging, sorting, and isolation 有权
标题翻译：高通量单细胞成像，分选和分离
公开(公告)号：US09165359B2
公开(公告)日：2015-10-20
申请号：US14595693
申请日：2015-01-13
申请人： Rajiv Bharadwaj , Bahram Fathollahi
发明人： Rajiv Bharadwaj , Bahram Fathollahi
IPC分类号： G06K9/00 , G06T7/00 , H04N7/18 , G01N33/53
CPC分类号： H04N5/2251 , B01L3/502761 , B01L2200/0636 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , C12M47/04 , C12N5/0081 , C12N5/0087 , G02B21/365 , G06T7/0012 , H04N5/2256 , H04N7/18
摘要： The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.
摘要翻译：本发明提供了一种隔离单个靶细胞的方法和装置。该装置包括主体结构，其包括主通道，收集通道和在第一流体结处流体耦合的废物通道。多个捕获通道与收集通道相交，每个捕获通道在与捕集通道与收集通道的交叉点相邻的位置处的直径小于单个靶细胞的直径。该装置还包括：成像系统，被配置为对主通道内的各个目标单元和非目标单元进行成像，从而产生成像数据; 处理器，被配置为执行所述成像数据的实时，多变量分析; 以及被配置为引导各个目标细胞的引导系统。压力源与收集通道，废物通道，第一侧通道和第二侧通道中的一个或多个流体连通。

146. 发明申请

US20120258487A1 MICROFLUIDIC PLATFORM FOR SYNTHETIC BIOLOGY APPLICATIONS 审中-公开
标题翻译：用于合成生物学应用的微流平台
公开(公告)号：US20120258487A1
公开(公告)日：2012-10-11
申请号：US13437727
申请日：2012-04-02
申请人： Chieh Chang , Rajiv Bharadwaj , Anup K. Singh , Aarthi Chandrasekaran , Nathan J. Hillson
发明人： Chieh Chang , Rajiv Bharadwaj , Anup K. Singh , Aarthi Chandrasekaran , Nathan J. Hillson
IPC分类号： C12P19/34 , C12N15/63 , C12M1/00 , C12N15/82 , C12Q1/04 , C12M1/40 , C12N11/00 , C12N15/85
CPC分类号： C12N15/1027 , C12N15/10 , C12N15/66
摘要： This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of said biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to for a sequenced order of droplets carrying different components of said biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of said biological construct to permit said components to react with each other in one or more reactions contributing to the assembly of said biological construct.
摘要翻译：本发明提供用于组装生物构建体（例如质粒，转化细胞等）的方法和组合物。在某些实施方案中，所述方法包括将所述生物构建体的单独组分封装在限制在流体通道中的流体液滴中; 任选地将来自不同流体通道的液滴混合以便在通道或室中携带所述生物构建体的不同组分的液滴的顺序排列; 并且任选地组合两个或更多个液滴，每个液滴包含所述生物构建体的不同组分，以允许所述组分在有助于所述生物构建体的组装的一个或多个反应中彼此反应。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式