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111. 发明授权

US06358919B1 Polymer compounds comprising glycosphingosine 失效
标题翻译：含有鞘氨醇的高分子化合物
公开(公告)号：US06358919B1
公开(公告)日：2002-03-19
申请号：US09109870
申请日：1998-07-06
申请人： Osamu Kanie , Chi-Huey Wong , Hiroshi Kamitakahara
发明人： Osamu Kanie , Chi-Huey Wong , Hiroshi Kamitakahara
IPC分类号： A61K3816
CPC分类号： A61K47/645
摘要： A polymer compound or a salt thereof which comprises a biodegradable polymer such as polyglutamic acid acetylated at N-terminal thereof containing a glycosphingolipid such as lysoganglioside GM3, and which exerts antiviral activity against variety of viruses and is useful as an active ingredient of a medicament.
摘要翻译：一种高分子化合物或其盐，其包含生物降解性聚合物，例如在其N末端乙酰化的聚谷氨酸，其含有鞘糖脂如溶血性神经节苷脂GM3，并且针对各种病毒发挥抗病毒活性，并且可用作药物的活性成分。

112. 发明授权

US06319695B1 Production of fucosylated carbohydrates by enzymatic fucosylation synthesis of sugar nucleotides; and in situ regeneration of GDP-fucose 失效
标题翻译：通过糖核苷酸的酶解岩藻糖基化合成生产岩藻糖基化碳水化合物; 和GDP-岩藻糖的原位再生
公开(公告)号：US06319695B1
公开(公告)日：2001-11-20
申请号：US07961076
申请日：1992-10-14
申请人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen , Kun-Chin Liu
发明人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen , Kun-Chin Liu
IPC分类号： C12P1918
CPC分类号： C07H5/02 , C07H3/06 , C07H11/04 , C07H13/04 , C07H13/08 , C07H15/18 , C07H19/20 , C12P19/18 , C12P19/26
摘要： This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.
摘要翻译：本发明考虑了碳水化合物，特别是岩藻糖基化碳水化合物的酶促生产的改进方法。还考虑了使用化学和酶学方法改进的1或2-磷酸糖基酯的合成。然后将磷酸化糖苷用于产生糖核苷酸，其又被用作供体糖用于受体碳水化合物的糖基化。本文特别优选使用所公开的岩藻糖基化方法。

113. 发明授权

US06255088B1 Enzymatic sulfation of biomolecules 失效
标题翻译：生物分子的酶硫酸化
公开(公告)号：US06255088B1
公开(公告)日：2001-07-03
申请号：US09393013
申请日：1999-09-10
申请人： Chi-Huey Wong , Michael D. Burkart
发明人： Chi-Huey Wong , Michael D. Burkart
IPC分类号： C12P1100
CPC分类号： C12N9/13
摘要： Enzymatic sulfations of biomolecules catalyzed by 3′-phosphoadenosine-5′-phosphosulfate (PAPS) dependent sulfotransferases are enhanced by coupling the sulfation reaction an enzymatic regeneration of PAPS. The PAPS is enzymatically regenerated from its hydrolysis product 3′,5′-diphosphate (PAP) using recombinant aryl sulfotransferase as the catalyst and aryl sulfate as the substrate. Biomolecules capable of enhanced sulfation by this method include carbohydrates, oligosaccharides, peptides, proteins, flavonoids, and steroids. When enzymatic sulfation and substrate regeneration steps are coupled, the desulfated aryl group produced in the regeneration step has a spectroscopic signature that can be employed for monitoring the progress of the sulfation of the biomolecule.
摘要翻译：通过将硫酸化反应与PAPS的酶促再生耦合，增强了由3'-磷酸腺苷-5'-磷酸硫酸酯（PAPS）依赖的磺基转移酶催化的生物分子的酶促硫酸化。使用重组芳基磺基转移酶作为催化剂和芳基硫酸盐作为底物，将PAPS从其水解产物3'，5'-二磷酸（PAP）酶促再生。能够通过该方法增强硫酸化的生物分子包括碳水化合物，寡糖，肽，蛋白质，类黄酮和类固醇。当酶促硫酸化和底物再生步骤偶联时，在再生步骤中产生的脱硫芳基具有可用于监测生物分子的硫酸化进程的光谱特征。

114. 发明授权

US6111084A Fuopeptide mimetics 失效
标题翻译：富肽模拟物
公开(公告)号：US6111084A
公开(公告)日：2000-08-29
申请号：US88411
申请日：1998-06-01
申请人： Chi-Huey Wong , Thomas F. J. Lampe
发明人： Chi-Huey Wong , Thomas F. J. Lampe
IPC分类号： C07H5/04 , A61K31/35 , A61K31/351 , A61K31/70 , A61K31/7008 , A61K31/7028 , A61K31/7034 , A61K31/7036 , A61K38/00 , A61P7/02 , A61P9/00 , A61P29/00 , A61P31/04 , A61P35/00 , A61P37/00 , A61P37/08 , C07D309/10 , C07H7/02 , C07H15/04 , C07H15/22 , C07K9/00
CPC分类号： C07H15/22 , C07H15/04 , C07H7/02 , C07K9/001 , A61K38/00
摘要： Bioactive fucopeptides and libraries of fucopeptides having advanced activities against P-selectin as sialyl Lewis X mimetics with IC.sub.50 values in the low mM range are synthesized by solid phase synthesis using para-acyloxymethylenzlidene acetal (p-AMBA) as an anchoring group. A rapid parallel synthesis is employed which proceeds with a bi-directional functionalization of glycosylated amino acid derivatives.
摘要翻译：通过使用对酰氧基甲基亚苄基缩醛（p-AMBA）作为锚定基团的固相合成，合成具有针对P-选择蛋白的先进活性的唾液酸肽作为唾液酸基路易斯X模拟物的生物活性uc肽和文库。使用快速并行合成，其进行糖基化氨基酸衍生物的双向官能化。

115. 发明授权

US5766887A Synthesis of 9-0-acetyl N-acetylneuraminic acid oligosaccharides 失效
标题翻译： 9-0-乙酰基N-乙酰神经氨酸寡糖的合成
公开(公告)号：US5766887A
公开(公告)日：1998-06-16
申请号：US703093
申请日：1996-08-26
申请人： Chi-Huey Wong , Shuichi Takayama
发明人： Chi-Huey Wong , Shuichi Takayama
IPC分类号： C07H15/10 , C12P19/26 , C12P19/44 , C12N9/24
CPC分类号： C07H15/10 , C12P19/26
摘要： Regioselective acetylation of the 9-hydroxyl group on N-acetylneuraminic acid is achieved enzymatically for producing oligosaccharides which contain a terminal N-acetylneuraminic acid moiety. This method provides access to O-acylated disialogangliosides as well as other N-acetyl-neuraminic acid oligosaccharides. These compounds are biologically and medicinally important and are difficult to obtain from nature or by chemical acylations. The methodology affords simple reaction conditions and minimal purification steps. In addition, the process affords good yields and the enzymes and reagents employed are commercially available with high stability and low costs.
摘要翻译：在N-乙酰神经氨酸的N-羟基上的区域选择性乙酰化被酶促用于制备含有末端N-乙酰神经氨酸部分的寡糖。该方法提供了获得O-酰化二唾液酸神经节苷脂以及其它N-乙酰神经氨酸寡糖。这些化合物在生物学和医学上是重要的，并且难以从自然或通过化学酰化获得。该方法提供简单的反应条件和最小的纯化步骤。此外，该方法提供良好的产率，所用的酶和试剂可以高稳定性和低成本商业获得。

116. 发明授权

US5759825A Method for synthesizing 2-ketoaldonic acids 失效
公开(公告)号：US5759825A
公开(公告)日：1998-06-02
申请号：US475777
申请日：1995-06-07
申请人： Chi-Huey Wong
发明人： Chi-Huey Wong
IPC分类号： C07H1/08 , C07H7/027 , C07H7/033 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02 , C12P7/40 , C07H1/00
CPC分类号： C12N9/88 , C07H1/08 , C07H7/027 , C07H7/033 , C12P19/02 , C12P7/58 , C12P9/00
摘要： 2-Ketoaldonic acid is synthesized by aldolase condensation reaction involving pyruvate and an aldose acceptor in the presence of excess pyruvate. After the reaction is substantially complete, the excess pyruvate is removed from the reaction mixture by treatment with pyruvate decarboxylase.

117. 发明授权

US5593887A Oligosaccharide enzyme substrates and inhibitors: methods and compositions 失效
标题翻译：寡糖酶底物和抑制剂：方法和组合物
公开(公告)号：US5593887A
公开(公告)日：1997-01-14
申请号：US476685
申请日：1995-06-07
申请人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
IPC分类号： C07H3/06 , C07H7/027 , C07H13/06 , C12N1/21 , C12N9/12 , C12N15/09 , C12P19/04 , C12P19/18 , C12P19/26 , C12R1/19 , C07H5/04 , C12N15/00
CPC分类号： C12N9/1241 , C12P19/04 , C12P19/18 , C12P19/26
摘要： Oligosacaharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.
摘要翻译：公开了作为底物的寡糖沙必利化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。还公开了糖基化的方法。用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

118. 发明授权

US5585261A KDO aldolase 失效
标题翻译： KDO醛缩酶
公开(公告)号：US5585261A
公开(公告)日：1996-12-17
申请号：US328739
申请日：1994-10-25
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02 , C12N9/86 , C12N1/20
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield.
摘要翻译：公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。

119. 发明授权

US5554771A 2-deoxy derivatives of N-acetyl neuraminic acid and their preparation 失效
标题翻译： N-乙酰神经氨酸的2-脱氧衍生物及其制备
公开(公告)号：US5554771A
公开(公告)日：1996-09-10
申请号：US515634
申请日：1995-08-16
申请人： Chi-Huey Wong , William J. Hennen
发明人： Chi-Huey Wong , William J. Hennen
IPC分类号： C07D309/10
CPC分类号： C07D309/10
摘要： The present invention relates to 2-deoxy-N-acetylneuraminic acid derivatives and methods for their preparation.
摘要翻译：本发明涉及2-脱氧-N-乙酰神经氨酸衍生物及其制备方法。

120. 发明授权

US5516665A N-acetylgalactosaminyl or N-acetylglucosaminyl transfer using N-acetylglucosaminyl-1-phosphate or N-acetylgalactosaminyl-1-phosphate as precursor and glycosyl-nucleotide regeneration 失效
标题翻译：使用N-乙酰葡糖胺基-1-磷酸或N-乙酰半乳糖胺基-1-磷酸作为前体的N-乙酰半乳糖胺基或N-乙酰葡糖胺转移，并进行糖基核苷酸再生
公开(公告)号：US5516665A
公开(公告)日：1996-05-14
申请号：US120179
申请日：1993-09-13
申请人： Chi-Huey Wong
发明人： Chi-Huey Wong
IPC分类号： C12P19/18 , C12P19/26 , C12P19/00 , C12P19/24 , C12P19/30
CPC分类号： C12P19/18 , C12P19/26
摘要： A one-pot glycosylation reaction is disclosed in which a N-acetylgalactosamine (GalNAc) or N-acetylglucosamine (GlcNAc) group is enzymatically transferred to an acceptor molecule. The starting glycoside is a N-acetylamino monosaccharide 1-phosphate that is enzymatically converted to its UDP derivative via UTP and a pyrophorylase. The formed UDP derivative is epimerized, and the epimerized UDP derivative is used in the enzyme-catalyzed glycosyl transfer. That enzyme-catalyzed glycosyl transfer to an acceptor releases UDP that is enzymatically converted to UTP for further conversion of the N-acetylamino monosaccharide 1-phosphate into its UDP derivative.
摘要翻译：公开了一锅糖基化反应，其中将N-乙酰半乳糖胺（GalNAc）或N-乙酰葡糖胺（GlcNAc）基团酶促转移到受体分子。起始糖苷是N-乙酰氨基单糖1-磷酸酯，其通过UTP和自发磷酸酶酶转化成其UDP衍生物。形成的UDP衍生物差向异构化，并且差向异构化的UDP衍生物用于酶催化的糖基转移。酶催化的糖基转移到受体释放UDP酶，其被酶转化成UTP，以进一步将N-乙酰氨基单糖1-磷酸转化成其UDP衍生物。

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