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101. 发明申请

US20040147030A1 Transgenic animals for monitoring water quality 审中-公开
标题翻译：用于监测水质的转基因动物
公开(公告)号：US20040147030A1
公开(公告)日：2004-07-29
申请号：US09863528
申请日：2001-05-22
发明人： Daniel W. Nebert
IPC分类号： G01N021/00
CPC分类号： G01N33/5308 , G01N21/763 , G01N33/186 , G01N33/5088 , G01N2520/00
摘要： The present invention provides methods and systems that uses transgenic zebrafish with an easily assessable reporter gene under the control of pollutant-inducible DNA response elements. Transgenic zebrafish, carrying pollution-inducible response elements, are placed in the water to be tested, and the contaminants become bioconcentrated (generally 1,000- to 40,000-fold, relative to the water) in the tissues of the fish thereby activating specific response elements, which up-regulate the LUC reporter gene. Fish are then removed from the test water and placed immediately in a luminometer cuvette and incubated with luciferin. Luciferin is rapidly taken up into the tissues of the fish, oxidized by luciferase, and light is produced. The luminescence is proportional to the environmental concentration of the pollutant (to which the fish had been exposed), which drives the expression of the LUC gene by means of the various DNA motifs. The luminescence is quantitated in the luminometer. In each response element-containing construct, a specific class of polluting chemicals, allowing for differential identification of pollutants in a complex mixture activates the expression of the LUC gene. This assay does not require killing the fish and allows for repeated analysis of the same site with the same fish. The sensitivity of the system can be manipulated by varying the sequence of the response element.
摘要翻译：本发明提供了在污染诱导型DNA应答元件控制下使用转基因斑马鱼与容易评估的报道基因的方法和系统。带有污染诱导型应答元件的转基因斑马鱼被放置在被测试的水中，并且污染物在鱼的组织中变得生物浓缩（相对于水）为生物浓缩物（通常为1,000至40,000倍），从而激活特定的应答元件，其上调LUC报告基因。然后将鱼从测试水中取出并立即放置在发光计比色皿中并与荧光素一起温育。萤光素被迅速吸收到鱼的组织中，被荧光素酶氧化，产生光。发光与污染物的环境浓度（鱼已暴露于其中）成比例，通过各种DNA图案驱动LUC基因的表达。在发光计中定量发光。在每个含有响应元素的构造中，特定类别的污染化学品允许复杂混合物中污染物的差异鉴定激活了LUC基因的表达。该测定不需要杀死鱼，并允许用相同的鱼重复分析相同的位点。可以通过改变响应元件的顺序来操纵系统的灵敏度。

102. 发明申请

US20010034039A1 Bioluminescent organism for detecting toxic substances 审中-公开
标题翻译：用于检测有毒物质的生物发光微生物
公开(公告)号：US20010034039A1
公开(公告)日：2001-10-25
申请号：US09791479
申请日：2001-02-23
发明人： Kyu-Ho Lee , Kyoung-Je Park , Sang-Jong Kim , Dong-Hun Lee , Deok Jin Jahng , Jang-Cheon Cho
IPC分类号： C12Q001/02 , C12N001/12
CPC分类号： C12R1/01 , C12Q1/02 , G01N2520/00
摘要： The present invention relates to a bioluminescent organism for detecting toxic substances, specifically to a microorganism YH9-RC which shows very sensitively change in the degree of luminescence when it contacts toxic substances, a method for detecting toxic substances using the changes in the degree of luminescence of the microorganism, and a kit for detecting toxic substances. Toxic substances of very low concentration can be detected using the luminescent organism YH9-RC of the present invention.
摘要翻译：本发明涉及用于检测有毒物质的生物发光生物体，特别涉及当与有毒物质接触时发光度非常敏感地变化的微生物YH9-RC，使用发光度的变化来检测有毒物质的方法的微生物，以及用于检测有毒物质的试剂盒。可以使用本发明的发光体YH9-RC检测非常低浓度的有毒物质。

103. 发明公开

KR1020120094346A 황산화미생물을 이용한 측정조 분리형 생태 독성탐지장치 有权
标题翻译：用硫酸氧化细菌检测水的毒性的装置
公开(公告)号：KR1020120094346A
公开(公告)日：2012-08-24
申请号：KR1020110013796
申请日：2011-02-16
申请人： 강원대학교산학협력단
发明人： 오상은 , 세드키하산
IPC分类号： C12M1/34 , C12Q1/04
CPC分类号： G01N33/1866 , C12Q1/02 , G01N2520/00
摘要： PURPOSE: A measurement tub decoupling-type ecotoxicology detecting device using sulfation microorganisms is provided to protect measurement value of conductivity and pH from air and bubbles generated when water sample is injected and to prevent interruption of sulfation microorganism activities due to pH of the water samples. CONSTITUTION: A measurement tub decoupling-type ecotoxicology detecting device(10) using sulfation microorganisms comprises an inlet port(120), an outlet port(140), a bioreactor which sulfated salt ionizes oxygen by the sulfation microorganisms, a measurement tub(310) in which water sample is inflowed by being connected with the microorganism reaction tub, pH measurement unit(320) and EC measurement unit(330) which respectively measure pH and conductivity of the water samples, and a controller which judges the toxicity of the water samples by comparing the conductivity and pH with the reference. A connection pipe is respectively connected to the outlet port of the bioreactor and inlet port of the measurement tub. The inlet port of the measurement tub is placed lower than the outlet port of the bioreactor so that the water sample can be inflowed into the measurement tub. A first barrier(380) in which one side is opened is perpendicularly installed inside the measurement tub and prevents generation of turnaround flow and vortex of the water sample.
摘要翻译：目的：提供使用硫酸化微生物的测量槽去耦型生态毒理学检测装置，以保护水样中注入的空气和气泡产生的电导率和pH值的测量值，并防止由于水样的pH而中断硫酸化微生物活动。构成：使用硫酸化微生物的测量槽去耦型生态毒理学检测装置（10）包括入口端口（120），出口端口（140），硫酸盐通过硫酸化微生物将氧气氧化的生物反应器，测量池（310）其中通过与分别测量水样品的pH和电导率的微生物反应槽，pH测量单元（320）和EC测量单元（330）连接而将水样品流入，以及判断水样品的毒性的控制器通过比较电导率和pH与参考。连接管分别连接到生物反应器的出口和测量槽的入口。测量槽的入口被放置在比生物反应器的出口低的位置，使得水样可以流入测量槽。将一侧打开的第一屏障（380）垂直地安装在测量桶内，并防止产生水样的周转流和涡流。

104. 发明公开

KR1020010086843A 독성물질 감지용 발광균주 失效
标题翻译：用于检测有毒物质的荧光微生物
公开(公告)号：KR1020010086843A
公开(公告)日：2001-09-15
申请号：KR1020000010763
申请日：2000-03-03
申请人： 이규호 , 김상종
发明人： 이규호 , 박경제 , 김상종 , 이동훈 , 장덕진 , 조장천
IPC分类号： C12N1/20
CPC分类号： C12R1/01 , C12Q1/02 , G01N2520/00
摘要： PURPOSE: Provided is a novel microorganism strain which has detectability for very low level of toxic substances. And a method of detecting the toxic substances using the same and a kit therefor are provided. CONSTITUTION: The novel microorganism strain, YH9-RC (KCTC 0730BP), changes the luminous level of light by reacting with toxic substances. The method of detecting the toxic substances is characterized by using aldehyde substrate to give luminescence to the microorganism. The aldehyde level used in the detection method is 0.01 to 0.015 weight. The toxic substances are selected from the group consisting of phenol, Al, As, Cd, Co, Cr(VI), Fe, Hg, Mn, Se, Zn and their mixture. The microorganism is prepared by the steps of: isolating gram negative strains from clean underground water on R2A agar plate; selecting Janthinobacterium lividium YH9 having highest sensitivity against phenol; and then introducing a luminous gene into the YH9 strain. Plasmid DNA comprising lux gene(pUTluxAB) is used to introduce the luminous gene into the YH9 strain, and the plasmid DNA is introduced into a receptor cell via a triparental conjugation.
摘要翻译：目的：提供一种新的微生物菌株，其具有非常低水平有毒物质的可检测性。并提供了使用该有毒物质检测有毒物质的方法及其试剂盒。构成：新型微生物菌株YH9-RC（KCTC 0730BP）通过与有毒物质反应来改变发光水平。检测有毒物质的方法的特征在于使用醛底物给微生物发光。检测方法中使用的醛水平为0.01〜0.015重量。有毒物质选自苯酚，Al，As，Cd，Co，Cr（VI），Fe，Hg，Mn，Se，Zn及其混合物。通过以下步骤制备微生物：在R2A琼脂平板上从干净的地下水中分离革兰氏阴性菌株; 选择对苯酚具有最高敏感性的黄曲霉YH9; 然后将发光基因引入YH9菌株。使用包含lux基因（pUTluxAB）的质粒DNA将发光基因引入YH9菌株，并通过三亲共轭将质粒DNA引入受体细胞。

105. 发明公开

EP2859331A2 KITS FOR DETECTING AND MONITORING ENDOCRINE DISRUPTING CHEMICALS (EDCS) 审中-公开
标题翻译： ZURÜBERWACHUNGEND ER ER。。。。。。。。。。。。
公开(公告)号：EP2859331A2
公开(公告)日：2015-04-15
申请号：EP13730754.2
申请日：2013-06-06
申请人： The United States of America, as represented by The Secretary, Department of Health and Human Services
发明人： HAGER, Gordon L. , STAVREVA, Diana A.
IPC分类号： G01N21/64 , G01N33/566 , G01N33/68
CPC分类号： G01N33/5035 , C07K14/435 , C07K14/705 , C07K2319/60 , G01N21/6486 , G01N33/5014 , G01N33/68 , G01N33/743 , G01N2520/00
摘要： Described herein are compositions, a system, and kits for detection of endocrine disruptor chemicals (EDCs) in environmental samples, such as samples of water including but not limited to waste water treatment plant effluent, using a live-cell fluorescence-based nuclear translocation reporter system. Upon binding of a ligand to a fluorescent-labeled reporter protein, the protein (and therefore the fluorescence) is translocated in a ligand level-dependent manner from the cytoplasm to the nucleus of live mammalian cells; this translocation is detectable as diffuse (cytoplasmic) fluorescence converting to localized, brightly fluorescent nuclei. The described kits can be used to reliably detect very low levels of EDC contamination, including in high throughput analysis systems as described.
摘要翻译：本文描述的是用于检测环境样品中的内分泌干扰物质（EDCs）的组合物，系统和试剂盒，例如使用基于活细胞荧光的核移位报告基因的水包括但不限于废水处理植物废水系统。当配体与荧光标记的报道蛋白结合时，蛋白质（因此荧光）以配体水平依赖的方式从细胞质转移到活哺乳动物细胞的细胞核; 这种易位可检测为扩散（细胞质）荧光转化为局部的，明亮的荧光核。所描述的套件可用于可靠地检测非常低水平的EDC污染，包括在所述的高通量分析系统中。

106. 发明公开

EP1978358A2 Sensor zur Erfassung eines toxischen oder gefährlichen Gasgemisches und Betriebsverfahren 审中-公开
标题翻译：传感器zur Erfassung eines toxischen odergefährlichenGasgemisches und Betriebsverfahren
公开(公告)号：EP1978358A2
公开(公告)日：2008-10-08
申请号：EP08103320.1
申请日：2008-04-02
申请人： SIEMENS AKTIENGESELLSCHAFT
发明人： Lehmann, Mirko , Fleischer, Maximilian , Simon, Elfriede
IPC分类号： G01N33/00 , C12M1/34 , C12Q1/02 , G01N27/414
CPC分类号： C12Q1/025 , G01N27/4141 , G01N2520/00
摘要： Sensor zur Erfassung eines Gasgemisches, welches im Wesentlichen aus Luft besteht und ein oder mehrere Gase enthält, die auf Lebewesen eine nachteilige Wirkung zeigen, bestehend aus: einem Sensorchip aus Silizium zur Auslesung von mindestens einem Signal, das an einer sensitiven Substanz bei vorliegendem einen oder mehreren Zielgasen im Messgas generiert wird, einer auf dem Sensorchip aufgebrachten sensitiven Substanz, die aus lebenden Zellen besteht, welche auf Zielgase ansprechen, einer Signalverarbeitungseinheit zur Auswertung der Signale des Si-Chips.
摘要翻译：传感器具有由硅制成的传感器芯片（1），用于选择在测量气体（4）中的一种或多种目标气体在敏感物质处产生的信号。敏感物质由活细胞（2）组成，其活化目标气体。敏感物质放在传感器芯片上。信号处理单元用于评估信号。还包括用于操作传感器的方法的独立权利要求。

107. 发明公开

EP1311156A4 FISH HATCHING METHOD AND APPARATUS 审中-公开
标题翻译： FISH犬种的方法和装置
公开(公告)号：EP1311156A4
公开(公告)日：2006-12-06
申请号：EP01970542
申请日：2001-08-16
申请人： US ARMY MED RES MAT COMMAND
发明人： SHEDD TOMMY R , WIDDER MARK W , HULL EUGENE
IPC分类号： G01N33/50
CPC分类号： G01N33/5088 , G01N33/5014 , G01N2520/00
摘要： A method and kit for conducting a rapid toxicity test. Methods and kits (Fig. 1) according to the invention include an animal or plant species in diapause.

108. 发明授权

EP1159442B1 AUTOMATIC WATER TOXICITY MEASURING APPARATUS 有权
标题翻译：自动，一种用来测量水毒性
公开(公告)号：EP1159442B1
公开(公告)日：2006-12-06
申请号：EP01942673.3
申请日：2001-01-17
申请人： Bioneer Corporation
发明人： PARK, HanOh, Bioneer Corporation , PARK, Hanee, Bioneer Corporation , CHOI, IlKyu, Bioneer Corporation
IPC分类号： C12Q1/00 , C12M1/00 , G01N21/76 , G01N21/13 , G01N33/18
CPC分类号： C12Q1/02 , G01N21/763 , G01N33/1826 , G01N33/1866 , G01N2520/00 , Y02A20/206
摘要： The present invention relates to an apparatus for automatic measuring of water toxicity. More specifically, the present invention provides an apparatus for automatic and continuous measuring of water toxicity and/or contamination using luminescent microorganisms living in freshwater.

109. 发明授权

EP1134585B1 Biosensor and method for the monitoring of herbicides 有权
标题翻译：生物传感器和方法，用于除草剂的监测
公开(公告)号：EP1134585B1
公开(公告)日：2006-02-01
申请号：EP01830148.1
申请日：2001-03-02
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE
发明人： Giardi, Maria Teresa , Leonardi, Chiara , Esposito, Dania , Margonelli, Andrea , Mattoo, Autar , Angelini, Giancarlo
IPC分类号： G01N33/68 , G01N33/53 , G01N33/50 , C12Q1/02
CPC分类号： G01N33/5097 , G01N2430/20 , G01N2520/00

110. 发明公开

EP1495131A2 PROCESS CONTROL BASED ON MICROBIOLOGICAL ACTIVITY 审中-公开
标题翻译：微生物学基于活动的过程控制
公开(公告)号：EP1495131A2
公开(公告)日：2005-01-12
申请号：EP03746505.1
申请日：2003-04-08
申请人： Nederlandse Organisatie voor Toegepast-Natuurwetenschappelijk Onderzoek TNO
发明人： MONTIJN, Roy, Christiaan , SCHUREN, Frank, Henri, Johan
IPC分类号： C12Q1/02 , C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/025 , C12Q2600/158 , G01N2520/00
摘要： The present invention relates to the determination of environmental conditions for the purpose of process control using microorganisms. The present invention uses the priciple that intrinsic changes in a microorganism after applying an external stimulus are characteristic of the nature of the stimulus the microorganism receives from outside. By measuring these intrinsic changes, microorganisms can be used as a specific sensor to determine environmental conditions, to determine the degree of change in these environmental conditions, to determine the nature and force of the stimulus and to be able to measure the effects of a diversity of stimuli on various processes an thus control these processes.

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