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    • 102. 发明申请
    • SELECTIVE LABELING AGENT FOR PHOSPHOPROTEOME ANALYSIS AND PHOSPHORYLATED SITE ANALYSIS
    • 选择性标记物质用于磷酸盐分析和磷酸盐化位点分析
    • US20050176093A1
    • 2005-08-11
    • US10907945
    • 2005-04-21
    • Yeong Hee AHNJong Shin YOOJin Young KIMKun CHO
    • Yeong Hee AHNJong Shin YOOJin Young KIMKun CHO
    • G01N33/68C12Q1/68C12P21/06G01N33/00
    • G01N33/6848A61K31/155G01N33/6803G01N33/6842G01N2458/15
    • Disclosed is a method of analyzing mass of the phosphoproteins or phosphopeptides and of analyzing phosphorylated positions at a phosphoprotein or phosphopeptide, comprising the steps of: 1) dephosphorylating at least one Ser and/or Thr residue of the phosphoprotein or phosphopeptide; 2) tagging the dephosphorylated amino acid residues with a tag having a R-L-G moiety wherein R is a nucleophilic functional group that selectively bind with dephosphorylated amino acid residues, G is selected from the group consisting of guanidine moiety or protected guanidine moiety such as a mono-N-protected guanidino group, a di-N,N′-protected guanidino group and an N′-protected guanidino group, and L is a linker linking the R and the G; and 3) subjecting the tagged proteins or peptides to mass spectrometry. The method is capable of precisely analyzing mass of phosphoproteins of trace amounts as well as positions of phosphoryated amino acids.
    • 公开了一种分析磷蛋白或磷酸肽的质量并分析磷蛋白或磷酸肽的磷酸化位置的方法,包括以下步骤:1)使磷酸蛋白或磷酸肽的至少一个Ser和/或Thr残基去磷酸化; 2)用具有RLG部分的标签标记去磷酸化的氨基酸残基,其中R是与去磷酸化的氨基酸残基选择性结合的亲核官能团,G选自胍部分或被保护的胍部分,例如单 - N-保护的胍基,二-N,N'保护的胍基和N'保护的胍基,L是连接R和G的连接基团; 和3)对标记的蛋白质或肽进行质谱分析。 该方法能够精确分析痕量的磷蛋白质量以及磷酸化氨基酸的位置。
    • 105. 发明授权
    • Laminography system having a view selector with prism
    • Laminography系统具有带棱镜的视图选择器
    • US06222903B1
    • 2001-04-24
    • US09261494
    • 1999-03-03
    • Hyeong-cheol KimJong-eun ByunJin-young KimChang-hyo KimHyung Seok ChoKuk-won KoYoung-jun Roh
    • Hyeong-cheol KimJong-eun ByunJin-young KimChang-hyo KimHyung Seok ChoKuk-won KoYoung-jun Roh
    • A61B600
    • G01N23/04
    • A laminography system includes a table, an X-ray source, an image intensifier, a view selector and a camera. The view selector has a prism for refracting a ray of light, and an adjusting part for adjusting the prism to a position in which images at a certain area of an image projecting plane of the image intensifier are received into the camera. The adjusting part has a first motor for rotating the prism along the circumferential direction of the image intensifier, and a second motor for rotating the prism in a direction perpendicular with respect to a radial direction of the image intensifier. Accordingly, the images at any areas of the image projecting plane of the image intensifier can be obtained. In addition, since an optical axis of the image passing through the prism and then received into the camera is in perpendicular relation with the camera, no image distortions occur.
    • 层析系统包括桌子,X射线源,图像增强器,视图选择器和照相机。 视角选择器具有用于折射光线的棱镜和用于将棱镜调节到图像增强器的图像投影平面的特定区域的图像被接收到相机中的位置的调节部分。 调整部具有用于沿图像增强器的圆周方向旋转棱镜的第一电动机和用于使棱镜沿与图像增强器的径向垂直的方向旋转的第二电动机。 因此,可以获得图像增强器的图像投影平面的任何区域的图像。 此外,由于通过棱镜然后被接收到相机中的图像的光轴与相机垂直,所以不会发生图像失真。