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101. 发明公开

EP2379753A2 SINGLE-CELL NUCLEIC ACID ANALYSIS 有权
标题翻译：单细胞分析NUCLEIC
公开(公告)号：EP2379753A2
公开(公告)日：2011-10-26
申请号：EP10732056.6
申请日：2010-01-13
申请人： Fluidigm Corporation
发明人： HAMILTON, Amy , LIN, Min , MIR, Alain , PIEPRZYK, Martin
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6881 , C12Q1/6844 , C12Q1/686 , C12Q2531/113 , C12Q2531/119 , C12Q2531/137 , C12Q2565/101 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , C12Q2600/178
摘要： The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

102. 发明公开

EP2254825A1 INTEGRATED CARRIER FOR MICROFLUIDIC DEVICE 审中-公开
标题翻译：集成设备支持微流体
公开(公告)号：EP2254825A1
公开(公告)日：2010-12-01
申请号：EP09712347.5
申请日：2009-02-20
申请人： Fluidigm Corporation
发明人： AMIN, Yusuf, D
IPC分类号： B81B1/00 , B81B7/00 , H01L21/44 , G01N27/00 , B01L3/00
CPC分类号： B01J19/0046 , B01L3/502707 , B01L9/527 , B01L2200/027 , B01L2200/04 , B01L2200/10 , B01L2200/12 , B01L2300/043 , B01L2300/123 , B01L2300/1805
摘要： A carrier for holding a microfluidic device includes a substrate with a plurality of wells, each well defining a volume of between 0.1 µl and 100 µl; a plurality of channels within the substrate wherein each well is in fluid communication with at least one of the plurality of channels; and a receiving portion for receiving a microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells. The carrier has a polymeric composition and/or an array of structural features that enhance its performance and compatibility with existing instrumentation.

103. 发明公开

EP2125219A2 HIGH PRECISION MICROFLUIDIC DEVICES AND METHODS 有权
标题翻译：霍奇拉西·威廉·弗洛伦斯
公开(公告)号：EP2125219A2
公开(公告)日：2009-12-02
申请号：EP08728100.2
申请日：2008-01-22
申请人： Fluidigm Corporation
发明人： COHEN, David, S. , WANG, Jing , MAY, Andrew, P. , JONES, Robert, C. , NASSEF, Hany
IPC分类号： B01L3/00
CPC分类号： B01L3/502738 , B01L3/5025 , B01L7/52 , B01L2200/0684 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0487 , B01L2400/0605 , B01L2400/0638 , B01L2400/0655 , B33Y80/00 , F16K99/0001 , F16K99/0026 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , Y10T137/0318 , Y10T137/85938
摘要： New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.
摘要翻译：新的高密度微流体装置和方法提供流体体积的精确计量和计量体积的有效混合。将第一溶液引入到与反应室流体连通的流动通道的段中。第二溶液流过该段，使得第一溶液移入反应室，并且第二溶液的体积进入室。然后可以分离室，并且可以启动和/或检测室内的反应。遗传分析的高通量方法可以以比以前更高的精度进行。

104. 发明公开

EP1535066A2 RECIRCULATING FLUIDIC NETWORK AND METHODS FOR USING THE SAME 审中-公开
标题翻译： REZIRKULATIONSFLUIDNETZ UND VERWENDUNGSVERFAHRENDAFÜR
公开(公告)号：EP1535066A2
公开(公告)日：2005-06-01
申请号：EP03742152.6
申请日：2003-06-23
申请人： Fluidigm Corporation
发明人： MANGER, Ian, D. , BARCO, Joseph, W. , NASSEF, Hany, R.
IPC分类号： G01N33/53
CPC分类号： B81B3/00 , B01L3/5025 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0861 , B01L2300/088 , B01L2300/0887 , B01L2400/0415 , B01L2400/0481 , B01L2400/0655 , F04B19/006 , F04B43/02 , F16K99/0001 , F16K99/0003 , F16K99/0026 , F16K99/0036 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , G01N33/5304 , G01N33/54366 , Y10T436/2575
摘要： The present invention provides microfluidic devices comprising elastomeric components and solid substrate component for attaching ligands and methods for conducting a variety of assays and high throughput screening. The elastomeric components comprise a plurality of first and second flow channels forming a plurality of intersecting areas, further capable of forming looped flow channels, and in fluid communication with the solid substrate surface, a plurality of control channels, a plurality of first and second control valves, and a plurality of loop forming control valves.
摘要翻译：本发明提供了微流体装置及其使用方法。特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。本发明的微流体装置包括用于在其表面上附着配体的弹性体组分和固体底物组分。弹性体层包括（a）多个第一流动通道; （b）多个第二流动通道，每个第二流动通道与每个所述第一流动通道相交并交叉，从而提供在所述第一流动通道和所述第二流动通道之间的交叉处形成的多个相交区域，其中所述多个第一流动通道和所述多个第二流动通道适于允许溶液流过其中，并且其中所述固体基底表面与所述多个第一流动通道和所述多个第二流动通道中的至少所述交叉区域流体连通，并且其中所述多个第二流动通道的第一流动通道和/或所述多个第二流动通道能够形成多个环形流动通道; （c）多个控制通道; （d）多个第一控制阀，每个第一控制阀相对于每个所述第一流动通道操作地设置以调节溶液通过所述第一流动通道的流动，其中每个所述第一控制阀包括第一控制通道和弹性体段，当所述第一流动通道响应于施加到所述第一控制通道的致动力而操作所述第一流动通道时所述第一流动通道可偏转进入或从所述第一流动通道缩回，所述弹性体段在定位在所述第一流动通道中时限制溶液流过其中; （e）多个第二控制阀，每个第二控制阀相对于每个所述第二流动通道操作地设置，以调节溶液通过所述第二流动通道的流动，其中每个所述第二控制阀包括第二控制通道和弹性体段，当所述第二流动通道响应于施加到所述第二控制通道的致动力而操作所述第二流动通道时，所述弹性体段被定位在所述第二流动通道中，所述第二流动通道限制溶液流过其中; （f）多个回路形成控制阀，每个操作地相对于每个所述第一和/或所述第二流动通道设置，以形成所述多个环形流动通道，其中每个所述回路成形控制阀包括回路成形控制通道以及弹性体段，其可偏转到所述第一和/或所述第二流动通道中或从所述第二流动通道中缩回，所述环形成控制阀响应于施加到所述环形成控制通道的致动力而操作，所述弹性体段定位在所述第一和和/或所述第二流动通道限制溶液流过其中，从而形成所述环形流动通道; 和（g）多个循环泵，并且其中每个循环泵相对于所述环形流动通道中的一个可操作地设置，使得通过每个所述环形流动通道的溶液的循环可以由所述循环泵之一调节。

105. 发明公开

EP1336097A2 MICROFLUIDIC DEVICE BASED SAMPLE INJECTION SYSTEM FOR ANALYTICAL DEVICES 审中-公开
标题翻译：样品注入系统上的微流体装置用于分析器件的基础
公开(公告)号：EP1336097A2
公开(公告)日：2003-08-20
申请号：EP01979990.7
申请日：2001-10-12
申请人： Fluidigm Corporation
发明人： MANGER, Ian, David , HAO, Cunsheng, Casey , UNGER, Marc, Alexander
IPC分类号： G01N24/00 , G01N30/00 , G01N33/00 , G03F7/00 , B01D59/44 , H01J49/40
CPC分类号： G01N1/10 , B01L3/0241 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L2200/027 , B01L2300/0816 , B01L2300/0887 , B01L2400/0481 , G01N30/7266 , H01J49/0018 , H01J49/165 , Y10T436/117497 , Y10T436/24 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/2575
摘要： This invention provides a microfluidic sample injection apparatus (14, 100) for injecting a fluid sample into an analytical device (120) and a method for using the same. The microfluidic sample injection apparatus (14, 100) comprises a microfluidic device (100) and an integrated sample injection capillary (14) which is in fluid communication with a fluid flow channel of the microfluidic device (100).

106. 发明申请

US20220413277A1 LASER CAPTURE MICRODISSECTION APPARATUS, SYSTEM AND METHOD 有权
公开(公告)号：US20220413277A1
公开(公告)日：2022-12-29
申请号：US17777894
申请日：2020-11-17
申请人： Fluidigm Corporation
发明人： Daaf Sandkuijl , Alan Carpino
IPC分类号： G02B21/16 , G02B21/32
摘要： A microscopy apparatus comprises a microscope comprising a stage configured to hold a tissue sample, a UV laser assembly configured to emit a UV laser beam to a viewing area of the tissue sample, and an IR laser assembly configured to emit an IR laser beam to the viewing area of the tissue sample. The UV and IR laser assemblies are oriented so as to emit the respective UV and IR laser beams in a same direction.

107. 发明授权

US11078476B2 Methods and kits for extracting nucleic acids from paraffin embedded samples 有权
公开(公告)号：US11078476B2
公开(公告)日：2021-08-03
申请号：US16105711
申请日：2018-08-20
申请人： Fluidigm Corporation
发明人： Jian Qin , Peilin Chen , Ramesh Ramakrishnan
IPC分类号： C12N15/10
摘要： This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

108. 发明授权

US10954560B2 Single-cell nucleic acids for high-throughput studies 有权
公开(公告)号：US10954560B2
公开(公告)日：2021-03-23
申请号：US16215489
申请日：2018-12-10
申请人： Fluidigm Corporation
发明人： Carolyn G. Conant , Tze Howe Charn , Jason A. A. West , Xiaohui Wang
IPC分类号： C12Q1/68 , B01L3/00 , C12Q1/6874 , C12Q1/6844
摘要： Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

109. 发明授权

US10578633B2 Methods and devices for analysis of defined multicellular combinations 有权
公开(公告)号：US10578633B2
公开(公告)日：2020-03-03
申请号：US14775685
申请日：2014-03-14
申请人： Fluidigm Corporation
发明人： Jason A. A. West , Brian Fowler
IPC分类号： G01N35/08 , G01N33/50 , G01N15/10 , G01N15/14 , C12M1/00 , G01N33/569 , B01L3/00 , C12M1/04
摘要： Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

110. 发明授权

US10501786B2 Nucleic acid encoding reactions 有权
公开(公告)号：US10501786B2
公开(公告)日：2019-12-10
申请号：US14723872
申请日：2015-05-28
申请人： Fluidigm Corporation
发明人： Megan Anderson , Peilin Chen , Brian Fowler , Robert C. Jones , Fiona Kaper , Ronald Lebofsky , Andrew May
IPC分类号： C12Q1/68 , C12Q1/6855 , C12N15/10 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
CPC分类号： C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
摘要： Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

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