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91. 发明申请

US20160326214A1 METHOD FOR THE PREPARATION OF BIOPOLYMERS 审中-公开
标题翻译：制备生物聚合物的方法
公开(公告)号：US20160326214A1
公开(公告)日：2016-11-10
申请号：US15109052
申请日：2015-01-05
申请人： UNITED UTILITIES PLC
发明人： Minh Son LE
IPC分类号： C07K1/30 , C07K1/14 , C12N15/10 , C07K9/00
CPC分类号： C07K1/30 , C02F1/36 , C02F1/54 , C02F11/00 , C02F11/12 , C02F2303/06 , C02F2303/26 , C07K1/14 , C07K9/00 , C12N15/1003 , Y02W10/45
摘要： The present invention relates to a method of preparing and separating biopolymers and biopolymer fractions useful for wastewater treatment applications from sewage sludge comprising the steps of disrupting the bacterial cell walls of bacteria present in the sewage sludge by at least 75% to release the intracellular contents of the bacterial cells and separating the biopolymers from any contaminants present.
摘要翻译：本发明涉及一种制备和分离生物聚合物和生物聚合物级分的方法，所述生物聚合物和生物聚合物级分可用于污水污泥的废水处理应用，包括以下步骤：将污水污泥中存在的细菌的细菌细胞壁破坏至少75％以释放细胞内含量细菌细胞并将生物聚合物与存在的任何污染物分离。

92. 发明申请

US20150368132A1 WASTEWATER TREATMENT SYSTEMS AND METHODS 审中-公开
标题翻译：废水处理系统和方法
公开(公告)号：US20150368132A1
公开(公告)日：2015-12-24
申请号：US14840841
申请日：2015-08-31
申请人： KUEHNLE AGROSYSTEMS, INC.
发明人： ADELHEID R. KUEHNLE , MARTIN JOHNSON , Robert J. Schurr
IPC分类号： C02F3/34
CPC分类号： C02F3/1284 , C02F3/20 , C02F3/28 , C02F3/322 , C02F3/34 , C02F3/341 , C02F3/345 , C02F2101/101 , C02F2101/105 , C02F2101/16 , C02F2101/20 , C02F2103/08 , C02F2103/10 , C02F2103/365 , C02F2301/024 , Y02E50/343 , Y02T50/678 , Y02W10/15 , Y02W10/37 , Y02W10/45
摘要： The present invention relates to improved wastewater treatment systems and methods. In one embodiment, the present invention utilizes microbial consortia and the final effluent of the treated wastewater is remarkably clean, and has low levels of hazardous chemicals, unwanted nutrient values and/or total solids. Advantageously, the wastewater treatment system and method of the present invention reduces the use of chemical compounds in the treatment process. Further, valuable biomasses can be produced from the wastewater using the treatment process of the present invention.
摘要翻译：本发明涉及改进的废水处理系统和方法。在一个实施方案中，本发明利用微生物菌群，并且经处理的废水的最终流出物非常干净，并且具有低水平的危险化学品，不需要的营养物质值和/或总固体。有利地，本发明的废水处理系统和方法减少了处理过程中化学化合物的使用。此外，使用本发明的处理方法可以从废水中产生有价值的生物质。

93. 发明授权

US09120686B2 Wastewater treatment methods 有权
标题翻译：污水处理方法
公开(公告)号：US09120686B2
公开(公告)日：2015-09-01
申请号：US14209724
申请日：2014-03-13
申请人： KUEHNLE AGROSYSTEMS, INC.
发明人： Adelheid R. Kuehnle , Martin Johnson , Robert J. Schurr
IPC分类号： C02F3/20 , C02F3/34 , C02F3/28
CPC分类号： C02F3/1284 , C02F3/20 , C02F3/28 , C02F3/322 , C02F3/34 , C02F3/341 , C02F3/345 , C02F2101/101 , C02F2101/105 , C02F2101/16 , C02F2101/20 , C02F2103/08 , C02F2103/10 , C02F2103/365 , C02F2301/024 , Y02E50/343 , Y02T50/678 , Y02W10/15 , Y02W10/37 , Y02W10/45
摘要： The present invention relates to improved wastewater treatment systems and methods. In one embodiment, the present invention utilizes microbial consortia and the final effluent of the treated wastewater is remarkably clean, and has low levels of hazardous chemicals, unwanted nutrient values and/or total solids. Advantageously, the wastewater treatment system and method of the present invention reduces the use of chemical compounds in the treatment process. Further, valuable biomasses can be produced from the wastewater using the treatment process of the present invention.
摘要翻译：本发明涉及改进的废水处理系统和方法。在一个实施方案中，本发明利用微生物菌群，并且经处理的废水的最终流出物非常干净，并且具有低水平的危险化学品，不需要的营养物质值和/或总固体。有利地，本发明的废水处理系统和方法减少了处理过程中化学化合物的使用。此外，使用本发明的处理方法可以从废水中产生有价值的生物质。

94. 发明申请

US20130040351A1 POLYHYDROXYALKANOATE PRODUCTION DURING WASTEWATER TREATMENT 审中-公开
标题翻译：废水处理过程中的聚羟基烷基酯生产
公开(公告)号：US20130040351A1
公开(公告)日：2013-02-14
申请号：US13206327
申请日：2011-08-09
申请人： HSIN-YING LIU , MICHAEL WAYNE FALK, JR.
发明人： HSIN-YING LIU , MICHAEL WAYNE FALK, JR.
IPC分类号： C12P7/40
CPC分类号： C02F3/12 , C02F3/1221 , C02F3/1263 , C02F3/34 , C02F2209/001 , C02F2209/10 , C12P7/42 , C12P7/625 , Y02W10/15 , Y02W10/45
摘要： A wastewater treatment process elicits microorganisms to convert a waste stream/organic resource to intracellular biopolymer polyhydroxyalkanoate (PHA). The process includes (i) waste stream/organic resource composition feed criteria, (ii) configuration coupled with operational parameters, and (iii) PHA-laden biomass separation and stabilization. A waste stream/organic resource capable of producing enhanced levels of PHA may be selected based on a combination of criteria, which may include short chain fatty acid concentration, protein concentration, polysaccharides concentration, and total suspended solids concentration. The waste stream is introduced into an aeration basin upon a specific configuration and operated under various parameter combinations for selecting/enriching microorganisms capable of producing PHA. The PHA-laden biomass is separated and stabilized for downstream PHA related product beneficial uses. The present process achieves concurrent wastewater treatment and PHA production, where PHA level (of more than 10% on a cell-weight basis) otherwise could not be obtained.
摘要翻译：废水处理过程引起微生物将废物流/有机物质转化为细胞内生物聚合物聚羟基链烷酸酯（PHA）。该过程包括（i）废物流/有机资源组合物进料标准，（ii）配置与操作参数，以及（iii）含PHA的生物质分离和稳定化。可以基于标准的组合来选择能够产生增强的PHA水平的废物流/有机资源，其可以包括短链脂肪酸浓度，蛋白质浓度，多糖浓度和总悬浮固体浓度。废物流根据特定的构型被引入曝气池并在各种参数组合下操作，用于选择/富集能够生产PHA的微生物。 PHA负载生物质被分离并稳定下游PHA相关产品的有益用途。本方法实现了同时进行的废水处理和PHA生产，其中PHA水平（以细胞重量计超过10％）不能获得。

95. 发明授权

US08202716B2 Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods 有权
公开(公告)号：US08202716B2
公开(公告)日：2012-06-19
申请号：US12927490
申请日：2010-11-15
申请人： David N. Thompson , William A. Apel , Vicki S. Thompson , David W. Reed , Jeffrey A. Lacey , Emily D. Henriksen
发明人： David N. Thompson , William A. Apel , Vicki S. Thompson , David W. Reed , Jeffrey A. Lacey , Emily D. Henriksen
IPC分类号： C12N9/42 , C12N9/14 , C12N9/00 , C12P21/06 , C08B37/00 , D21C1/00 , C02F3/34 , C07H21/04 , C07K1/00
CPC分类号： C12Y302/01055 , C12N9/2402 , C12N15/52 , Y02W10/45
摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

96. 发明申请

US20120015407A1 Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods 有权
标题翻译：嗜热和热嗜酸性生物聚合物降解基因和酸性脂肪酸杆菌和相关生物的酶，方法
公开(公告)号：US20120015407A1
公开(公告)日：2012-01-19
申请号：US12927490
申请日：2010-11-15
申请人： David N. Thompson , William A. Apel , Vicki S. Thompson , David W. Reed , Jeffrey A. Lacey , Emily D. Henriksen
发明人： David N. Thompson , William A. Apel , Vicki S. Thompson , David W. Reed , Jeffrey A. Lacey , Emily D. Henriksen
IPC分类号： C12N9/24 , C12P19/14
CPC分类号： C12Y302/01055 , C12N9/2402 , C12N15/52 , Y02W10/45
摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.
摘要翻译：提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列。还提供了使用分离的至少部分降解，切割或除去多糖，木质纤维素，纤维素，半纤维素，木质素，淀粉，几丁质，聚羟基丁酸酯，异木聚糖，糖苷，木聚糖，葡聚糖，半乳聚糖或甘露聚糖装饰基团的方法和/或编码来自酸性脂多酸杆菌的多肽的纯化多肽和核酸序列。

97. 发明授权

US07932063B2 Treatment of biomass to obtain fermentable sugars 有权
标题翻译：处理生物质获得可发酵糖
公开(公告)号：US07932063B2
公开(公告)日：2011-04-26
申请号：US11402757
申请日：2006-04-12
申请人： James B. Dunson, Jr. , Melvin Tucker , Richard Elander , Susan M. Hennessey
发明人： James B. Dunson, Jr. , Melvin Tucker , Richard Elander , Susan M. Hennessey
IPC分类号： C12P7/00 , C12P19/00 , C12P19/14
CPC分类号： C12M45/02 , C12M27/02 , C12M27/20 , C12M45/09 , C12P7/08 , C12P19/02 , C12P2201/00 , Y02E50/17 , Y02E50/343 , Y02W10/45
摘要： Biomass is pretreated using a low concentration of aqueous ammonia at high biomass concentration. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.
摘要翻译：使用低浓度的氨水在高生物质浓度下预处理生物质。预处理的生物质被糖化酶联合体进一步水解。通过糖化释放的发酵糖可用于通过发酵生产目标化学品。

98. 发明申请

US20100227381A1 ENHANCED BIODEGRADATION OF NON-AQUEOUS PHASE LIQUIDS USING SURFACTANT ENHANCED IN-SITU CHEMICAL OXIDATION 审中-公开
标题翻译：使用表面活性剂增强的现场化学氧化增强非水相液体的生物降解
公开(公告)号：US20100227381A1
公开(公告)日：2010-09-09
申请号：US12670373
申请日：2008-07-23
申请人： George E. Hoag , John Collins
发明人： George E. Hoag , John Collins
IPC分类号： A62D3/02 , C02F1/72 , C02F1/70 , C02F3/30 , B09C1/08 , B09C1/10 , G01N33/24
CPC分类号： B09C1/02 , B09C1/00 , B09C1/08 , B09C1/10 , C02F2101/36 , C02F2103/06 , Y02W10/45
摘要： A method for in-situ reduction of contaminants in soil that uses chemical oxidation and biodegradation.
摘要翻译：一种利用化学氧化和生物降解原位还原污染物的方法。

99. 发明授权

US07473364B2 Multivalent metal ion management for low sludge processes 失效
标题翻译：用于低污泥处理的多价金属离子管理
公开(公告)号：US07473364B2
公开(公告)日：2009-01-06
申请号：US11683162
申请日：2007-03-07
申请人： Mohammad M. Abu-Orf
发明人： Mohammad M. Abu-Orf
IPC分类号： C02F3/31
CPC分类号： C02F3/12 , C02F1/5236 , C02F1/68 , C02F3/1221 , C02F11/02 , C02F11/04 , C02F2209/04 , C02F2301/046 , Y02W10/15 , Y02W10/27 , Y02W10/45
摘要： A method for managing multivalent metal ion concentrations in low-yield wastewater treatment processes. The method includes combining wastewater containing BOD with bacteria-laden sludge in a mainstream reactor to form a mixed liquor, separating the mixed liquor into a clear effluent and an activated sludge stream, returning a first portion of the activated sludge stream to the mainstream reactor, processing a second portion of the activated sludge stream in a sidestream bioreactor, returning at least a portion of the activated sludge stream in the sidestream bioreactor to the mainstream reactor, and adding multivalent metal ions to at least one of the wastewater, the mixed liquor, the activated sludge stream and combinations thereof to reduce the generation of waste activated sludge.
摘要翻译：一种在低产废水处理过程中管理多价金属离子浓度的方法。该方法包括在主流反应器中将含有BOD的废水与含细菌污泥混合形成混合液，将混合液分离成澄清的流出物和活性污泥流，将第一部分活性污泥流返回主流反应器，在侧流生物反应器中处理活性污泥流的第二部分，将侧流生物反应器中的至少一部分活性污泥流返回主流反应器，并将多价金属离子添加到至少一种废水，混合液，活性污泥流及其组合以减少废活性污泥的产生。

100. 发明申请

US20050269261A1 Apparatus and methods for forming microcultures within porous media 有权
标题翻译：在多孔介质中形成微量培养物的装置和方法
公开(公告)号：US20050269261A1
公开(公告)日：2005-12-08
申请号：US11122700
申请日：2005-05-05
申请人： Kerry Sublette
发明人： Kerry Sublette
IPC分类号： C02F1/28 , C02F3/00 , C02F3/10
CPC分类号： C02F1/288 , C02F1/283 , C02F3/10 , C02F3/108 , C02F3/348 , C02F2003/003 , Y02W10/15 , Y02W10/45
摘要： Highly porous, beads are comprised of a polymer and a second compound mixed into it. The second compound, an amendment, is either a nutrient or a compound having high affinity to one or more nutrients. A plurality of these beads may be exposed to an aqueous environment, usually a body of water. Bacteria and other microorganisms rapidly enter and remain within the nutrient filled interior space of the beads. Any of a number of various detection methods may then be used to characterize, detect and/or identify the microorganisms.
摘要翻译：高度多孔的珠由聚合物和混入其中的第二化合物组成。第二种化合物，一种修正，是对一种或多种营养素具有高亲和力的营养物质或化合物。多个这些珠可以暴露于水性环境，通常是水体。细菌和其他微生物迅速进入并保留在珠的营养填充内部空间内。然后可以使用多种各种检测方法中的任何一种来表征，检测和/或识别微生物。

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