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91. 发明授权

US06586182B1 Methods and compositions for polypeptide engineering 失效
公开(公告)号：US06586182B1
公开(公告)日：2003-07-01
申请号：US09693389
申请日：2000-10-20
申请人： Phillip A. Patten , Willem P. C. Stemmer
发明人： Phillip A. Patten , Willem P. C. Stemmer
IPC分类号： C12Q168
CPC分类号： C12N15/1031 , A61K38/45 , A61K48/00 , C07K14/43595 , C07K14/545 , C07K14/56 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N7/00 , C12N9/0069 , C12N9/1007 , C12N9/16 , C12N9/86 , C12N15/10 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12N15/67 , C12N2795/14043 , C12N2795/14143 , C12Q1/6811 , C12Q1/6853 , C12Q1/686 , C40B40/02 , C40B40/08 , C40B50/06 , G01N33/68 , G01N33/6845
摘要： Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.

92. 发明授权

US06573098B1 Nucleic acid libraries 失效
标题翻译：核酸文库
公开(公告)号：US06573098B1
公开(公告)日：2003-06-03
申请号：US09240307
申请日：1999-01-29
申请人： Willem P. C. Stemmer
发明人： Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C40B40/02 , C07K14/43595 , C07K14/545 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N9/2471 , C12N9/86 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12Q1/68 , C12Q1/6811 , C12Y302/01023 , C12Y305/02006 , H01L39/228 , C12Q2525/179
摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
摘要翻译：描述了随机断裂后DNA重组的方法及其通过体外或体内重组诱变核酸序列的应用。特别地，描述了用于生产编码突变蛋白的核酸片段或多核苷酸的方法。本发明还涉及一种反复循环诱变，洗牌和选择的方法，其允许蛋白质的体外或体内的定向分子进化。

93. 发明授权

US06518065B1 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination 失效
公开(公告)号：US06518065B1
公开(公告)日：2003-02-11
申请号：US09696313
申请日：2000-10-25
申请人： Willem P. C. Stemmer
发明人： Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C40B40/02 , C07K14/43595 , C07K14/545 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N9/2471 , C12N9/86 , C12N15/1027 , C12N15/1037 , C12N15/52 , C12N15/64 , C12Q1/68 , C12Q1/6811 , C12Y302/01023 , C12Y305/02006
摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.

94. 发明授权

US06482647B1 Evolving susceptibility of cellular receptors to viral infection by recursive recombination 失效
标题翻译：细胞受体对递归重组病毒感染的易感性
公开(公告)号：US06482647B1
公开(公告)日：2002-11-19
申请号：US09724067
申请日：2000-11-28
申请人： Willem P. C. Stemmer
发明人： Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C12N15/1058 , C12N15/1027 , C12N15/1034 , C12N15/52 , C12N15/64
摘要： The invention provides a number of strategies for transferring and/or evolving gene(s) associated with cellular DNA uptake so that they confer or enhance DNA-uptake capacity of a recipient cell. Evolution is achieved by recursive cycles of recombination and screening/selection. One such strategy entails evolving genes that confer competence in one species to confer either greater competence in that species, or comparable or greater competence in a second species. Another strategy entails evolving genes for use as components of cloning vector to confer enhanced uptake of the vector. Other strategies entail evolving viral receptors, viruses, and genes that mediate conjugal transfer.
摘要翻译：本发明提供了用于转移和/或发展与细胞DNA摄取相关的基因的许多策略，使得它们赋予或增强受体细胞的DNA吸收能力。通过重组和筛选/选择的递归周期来实现进化。一种这样的策略需要演变基因，赋予一种物种的能力，以赋予该物种更大的能力，或者在第二种物种中具有相当或更大的能力。另一种策略需要进化基因，用作克隆载体的组分以赋予载体增强的摄取。其他策略需要发展介导共轭转移的病毒感受器，病毒和基因。

95. 发明授权

US06479652B1 Oligonucleotide mediated nucleic acid recombination 有权
公开(公告)号：US06479652B1
公开(公告)日：2002-11-12
申请号：US09626595
申请日：2000-07-27
申请人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
IPC分类号： C07H2102
CPC分类号： C12N15/11 , A61K39/00 , B01J2219/00689 , C07K14/005 , C07K14/505 , C07K14/535 , C12N9/16 , C12N15/1031 , C12N15/1034 , C12N2740/16122
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

96. 发明授权

US06455253B1 Methods and compositions for polypeptide engineering 失效
公开(公告)号：US06455253B1
公开(公告)日：2002-09-24
申请号：US09559565
申请日：2000-04-27
申请人： Phillip A. Patten , Willem P. C. Stemmer
发明人： Phillip A. Patten , Willem P. C. Stemmer
IPC分类号： C12Q168
CPC分类号： C12N15/1031 , A61K38/45 , A61K48/00 , C07K14/43595 , C07K14/545 , C07K14/56 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N7/00 , C12N9/0069 , C12N9/1007 , C12N9/16 , C12N9/86 , C12N15/10 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12N15/67 , C12N2795/14043 , C12N2795/14143 , C12Q1/6811 , C12Q1/6853 , C12Q1/686 , C40B40/02 , C40B40/08 , C40B50/06 , G01N33/68 , G01N33/6845
摘要： Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.

97. 发明授权

US06423542B1 Oligonucleotide mediated nucleic acid recombination 失效
公开(公告)号：US06423542B1
公开(公告)日：2002-07-23
申请号：US09626930
申请日：2000-07-27
申请人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
IPC分类号： C12N1500
CPC分类号： C12N15/11 , A61K39/00 , B01J2219/00689 , C07K14/005 , C07K14/505 , C07K14/535 , C12N9/16 , C12N15/1031 , C12N15/1034 , C12N2740/16122
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

98. 发明授权

US06391640B1 Methods and compositions for cellular and metabolic engineering 有权
标题翻译：细胞和代谢工程的方法和组成
公开(公告)号：US06391640B1
公开(公告)日：2002-05-21
申请号：US09188777
申请日：1998-11-09
申请人： Jeremy Minshull , Willem P. C. Stemmer
发明人： Jeremy Minshull , Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C40B40/02 , B07C2501/009 , C07K14/43595 , C07K14/545 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N9/2471 , C12N9/86 , C12N15/1027 , C12N15/1037 , C12N15/1058 , C12N15/1093 , C12N15/52 , C12N15/64 , C12Q1/6811 , C12Y302/01023
摘要： The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.
摘要翻译：本发明一般涉及新代谢途径的演变以及通过本文中称为递归序列重组的方法增强生物处理。递归序列重组需要执行重组和筛选或选择的“进化”个体基因，整个质粒或病毒，多基因簇或甚至全基因组的迭代循环。这些技术不需要传统的代谢工程方法所需的广泛的分析和计算。

99. 发明授权

US06365408B1 Methods of evolving a polynucleotides by mutagenesis and recombination 有权
标题翻译：通过诱变和重组进化多核苷酸的方法
公开(公告)号：US06365408B1
公开(公告)日：2002-04-02
申请号：US09477763
申请日：2000-01-04
申请人： Willem P. C. Stemmer
发明人： Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C12Q1/6811 , C12N15/1058 , C40B40/08 , C40B50/06
摘要： A method of mutating a polynucleotide such that it has a desired or improved functional property is disclosed.
摘要翻译：公开了一种使多核苷酸突变以使其具有所需或改进的功能性质的方法。

100. 发明授权

US06335160B1 Methods and compositions for polypeptide engineering 失效
公开(公告)号：US06335160B1
公开(公告)日：2002-01-01
申请号：US08769062
申请日：1996-12-18
申请人： Phillip A. Patten , Willem P. C. Stemmer
发明人： Phillip A. Patten , Willem P. C. Stemmer
IPC分类号： C07H2102
CPC分类号： C12N15/1031 , A61K38/45 , A61K48/00 , C07K14/43595 , C07K14/545 , C07K14/56 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N7/00 , C12N9/0069 , C12N9/1007 , C12N9/16 , C12N9/86 , C12N15/10 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12N15/67 , C12N2795/14043 , C12N2795/14143 , C12Q1/6811 , C12Q1/6853 , C12Q1/686 , C40B40/02 , C40B40/08 , C40B50/06 , G01N33/68 , G01N33/6845
摘要： Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.

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