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    • 95. 发明申请
    • HUMAN PROTEIN C EXPRESSION VECTOR
    • 人类蛋白C表达载体
    • WO1992013079A1
    • 1992-08-06
    • PCT/JP1992000070
    • 1992-01-28
    • TEIJIN LIMITED
    • TEIJIN LIMITEDSUZUKI, MasahikoWAKABAYASHI, KenjiTAKAZAWA, YoshiharuSUMI, Yoshihiko
    • C12N15/57
    • C12N9/6464C12N15/67C12Y304/21069
    • An object of the present invention is to provide DNA fragments encoding human protein C and human protein C expression vectors including the same. The object is attained by removing arbitrary n (n = 1 to 6) introns from 7 introns distributing between 8 exons in human protein C gene, ligating the remaining before and behind sequences to form novel DNA fragments encoding human protein C and preparing expression vectors including the same and mammalian cells transformed with said vectors. Another object of the present invention is to provide DNA fragments of increased expression efficiency by incorporating a useful DNA fragment into the introns remaining in the DNA fragments. They are useful for production of human protein C.
    • 本发明的目的是提供编码人蛋白C的DNA片段和包含该蛋白质的人蛋白C表达载体。 目的是通过从人蛋白C基因中8个外显子之间分布的7个内含子中去除任意n(n = 1至6)内含子,连接剩余的序列之前和之后,形成编码人蛋白C的新型DNA片段,并制备表达载体,包括 用所述载体转化的相同和哺乳动物细胞。 本发明的另一个目的是通过将有用的DNA片段并入到残留在DNA片段中的内含子中来提供增加的表达效率的DNA片段。 它们可用于生产人类蛋白质C.
    • 98. 发明申请
    • PLASTER
    • 石膏
    • WO1991016044A1
    • 1991-10-31
    • PCT/JP1991000541
    • 1991-04-23
    • TEYSAN PHARMACEUTICALS CO., LTD.TEIJIN LIMITEDHIDAKA, OsafumiMURAKAMI, Satoshi
    • TEYSAN PHARMACEUTICALS CO., LTD.TEIJIN LIMITED
    • A61K09/70
    • A61K9/703A61F13/00063A61F13/0206A61F13/0223A61F13/0226A61F13/023A61F13/0253A61F13/0256A61F2013/00663A61F2013/00868A61F2013/00906A61K9/7061A61K9/7084
    • A plaster consisting of a film layer formed of a film wherein the thickness thereof is 0.5 - 4.9 mu m, the strengths in two directions intersecting substantially perpendicularly are 8 - 85 g/mm, respectively, the elongations in two directions intersecting substantially perpendicularly are 30 - 150 %, respectively, and, an elongation ratio of one direction to the other is 1.5 - 5.0 (provided that, when the elongations in the two directions are not equal, a smaller elongation is used as a denominator) and an adhesive layer (a) formed of an adhesive agent containing a percutaneous absorptive pharmaceutical, laminated on one of the surfaces of the film layer and having a thickness of 2 - 60 mu m. The possibility of skin poisoning is decreased and a pharmaceutical of a clinically effective amount can be percutaneously absorbed. Further, it is preferable to provide a layer such as a planar support member. For example, a plaster consisting of the film layer (3) and the adhesive layer (4), wherein a planar support member (1) and a peelable film (5) are further laminated through an adhesive layer (b) (2), is excellent to handle.
    • 由厚度为0.5-4.9μm的膜形成的薄膜层,基本上垂直相交的两个方向的强度分别为8〜85g / mm 2,两个方向上大致垂直相交的伸长率为30 - 150%,并且一个方向相对于另一个方向的伸长率为1.5-5.0(条件是当两个方向上的伸长率不相等时,使用较小的伸长率作为分母)和粘合剂层 a)由含有经皮吸收药物的粘合剂形成,层压在膜层的一个表面上,厚度为2-60μm。 皮肤中毒的可能性降低,可以经皮吸收临床有效量的药物。 此外,优选地,提供诸如平面支撑构件的层。 例如,通过粘合剂层(b)(2)进一步层压由薄膜层(3)和粘合剂层(4)组成的石膏,其中平面支撑构件(1)和可剥离薄膜(5) 非常好处理。