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    • 94. 发明授权
    • Assays and other reactions involving droplets
    • 测定和其他涉及液滴的反应
    • US09029085B2
    • 2015-05-12
    • US12529926
    • 2008-03-07
    • Jeremy AgrestiLiang-Yin ChuDavid A. WeitzJin-Woong KimAmy RowatMorten SommerGautam DantasGeorge Church
    • Jeremy AgrestiLiang-Yin ChuDavid A. WeitzJin-Woong KimAmy RowatMorten SommerGautam DantasGeorge Church
    • C12Q1/68C12P19/34B01J13/00B01F3/08B01F13/00
    • C12P19/34B01F3/0807B01F3/0811B01F13/0062B01F13/0071B01F2215/0037B01J13/0052B01J13/0065B01L3/502784C12Q1/6834C12Q1/6848C12Q1/686G01N15/1404G01N2015/1006
    • The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
    • 本发明通常涉及液滴和/或乳液,例如多重乳液。 在一些情况下,液滴和/或乳液可用于测定中,并且在某些实施方案中,液滴或乳液可被硬化以形成凝胶。 在一些方面,可以使用凝胶进行异质测定。 例如,液滴可以被硬化以形成凝胶,其中液滴包含细胞,DNA或其它合适的物质。 凝胶可以暴露于反应物,并且反应物可以以某种方式与凝胶和/或与细胞,DNA等相互作用。 例如,反应物可以扩散通过凝胶,或者硬化的颗粒可以液化以形成液体状态,允许反应物与细胞相互作用。 作为具体实例,包含在凝胶颗粒内的DNA可以进行PCR(聚合酶链式反应)扩增,例如通过使用能够在形成凝胶时结合凝胶的PCR引物。 当使用PCR扩增DNA时,一些DNA将通过PCR引物与凝胶结合。 PCR反应后,未结合的DNA可以从凝胶中去除,例如通过扩散或洗涤。 因此,可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。
    • 98. 发明授权
    • Washing method of washing machine and apparatus thereof
    • 洗衣机的洗涤方法及其装置
    • US07934278B2
    • 2011-05-03
    • US10751978
    • 2004-01-07
    • Soo-Young OhKyung-Chul WooJin-Woong KimSi-Moon Jeon
    • Soo-Young OhKyung-Chul WooJin-Woong KimSi-Moon Jeon
    • D06B19/00D06B1/02
    • D06F39/008D06F35/006D06F2202/085D06F2204/086
    • Disclosed are a washing method of a washing machine capable of preventing a phenomenon that remaining detergent is collected into a space where a heater is positioned or a specific space during a washing operation of laundry, capable of minimizing a generation of washing water unnecessarily supplied to the washing machine, and capable of fast heating corresponding laundry before a washing operation for an efficient washing operation, and an apparatus thereof. To this end, the method comprises the steps of: generating steam by heating washing water supplied into a washing machine; injecting the generated steam into laundry inside of a drum of the washing machine; and rotating the drum after injecting the steam into the laundry and thereby washing the laundry.
    • 公开了一种洗衣机的洗涤方法,其能够防止洗涤剂在洗涤时的洗涤操作期间将残留的洗涤剂收集到加热器所在的空间或特定空间中的现象,能够将不必要地供给到洗涤剂的洗涤水的产生最小化 洗衣机,并且能够在用于高效洗涤操作的洗涤操作之前快速加热相应的衣物及其装置。 为此,该方法包括以下步骤:通过加热供应到洗衣机中的洗涤水来产生蒸汽; 将产生的蒸汽注入到洗衣机的滚筒内的衣物内; 并且在将蒸汽注入衣物中之后旋转滚筒,从而洗涤衣物。
    • 100. 发明申请
    • ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS
    • 测量和其他涉及倾销的反应
    • US20100136544A1
    • 2010-06-03
    • US12529926
    • 2008-03-07
    • Jeremy AgrestiLiang-Yin ChuDavid A. WeitzJin-Woong KimAmy RowatMorten SommerGautam DantasGeorge Church
    • Jeremy AgrestiLiang-Yin ChuDavid A. WeitzJin-Woong KimAmy RowatMorten SommerGautam DantasGeorge Church
    • C12Q1/68C12Q1/04
    • C12P19/34B01F3/0807B01F3/0811B01F13/0062B01F13/0071B01F2215/0037B01J13/0052B01J13/0065B01L3/502784C12Q1/6834C12Q1/6848C12Q1/686G01N15/1404G01N2015/1006
    • The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
    • 本发明通常涉及液滴和/或乳液,例如多重乳液。 在一些情况下,液滴和/或乳液可用于测定中,并且在某些实施方案中,液滴或乳液可被硬化以形成凝胶。 在一些方面,可以使用凝胶进行异质测定。 例如,液滴可以被硬化以形成凝胶,其中液滴包含细胞,DNA或其它合适的物质。 凝胶可以暴露于反应物,并且反应物可以以某种方式与凝胶和/或与细胞,DNA等相互作用。 例如,反应物可以扩散通过凝胶,或者硬化的颗粒可以液化以形成液体状态,允许反应物与细胞相互作用。 作为具体实例,包含在凝胶颗粒内的DNA可以进行PCR(聚合酶链式反应)扩增,例如通过使用能够在形成凝胶时结合凝胶的PCR引物。 当使用PCR扩增DNA时,一些DNA将通过PCR引物与凝胶结合。 PCR反应后,未结合的DNA可以从凝胶中去除,例如通过扩散或洗涤。 因此,可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。