专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

91. 发明专利

NZ575237A NOVEL ENGINEERED MICROORGANISM PRODUCING HOMO-SUCCINIC ACID AND METHOD FOR PREPARING SUCCINIC ACID USING THE SAME 未知
公开(公告)号：NZ575237A
公开(公告)日：2011-11-25
申请号：NZ57523707
申请日：2007-07-25
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , LIM SUNG WON , SONG HYOHAK
IPC分类号： C12N1/21
摘要： Disclosed is a homofermentative mutant microorganism that lacks the lactate dehydrogenase gene (ldhA), a phosphotransacetylase gene (pta), and an acetate kinase gene (ackA) and retains a pyruvate formate-lyase gene (pfl). The microorganism produces a higher yield of succinic acid and minimises or eliminates the production of the organic acids lactic acid, acetic acid, formic acid and pyruvic acid under anaerobic conditions. The microorganism can be selected from the genera Mannheimia, Actinobacillus and Anaerobiospirillum, in particular the microorganism is M. succinicproducens (KCTC10973BP). The microorganism can be used in methods for the production of succinic acid.

92. 发明专利

AU2005332120B2 Method for preparing a target protein using the sHSPs 未知
公开(公告)号：AU2005332120B2
公开(公告)日：2010-08-26
申请号：AU2005332120
申请日：2005-05-23
申请人： KOREA ADVANCED INST SCI & TECH
发明人： HAN MEE JUNG , LEE SANG YUP
IPC分类号： C07K1/14
摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sIISPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.

93. 发明专利

GB2440490B Method for preparing a target protein using the sHSPs 未知
公开(公告)号：GB2440490B
公开(公告)日：2010-06-02
申请号：GB0722849
申请日：2005-05-23
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG
IPC分类号： C07K1/14
摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sIISPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.

94. 发明专利

AU2009263165A1 Method for preparing polylactate and copolymer thereof using a mutant microorganism with enhanced polylactate, and the copolymer producing capability thereof 未知
公开(公告)号：AU2009263165A1
公开(公告)日：2009-12-30
申请号：AU2009263165
申请日：2009-06-24
申请人： KOREA ADVANCED INST SCI & TECH , LG CHEMICAL LTD
发明人： YANG TAEK HO , LEE SANG YUP , KIM TAE WAN , JUNG YU KYUNG , PARK SI JAE
IPC分类号： C08G63/91
摘要： Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.

95. 发明专利

DE112005003358T5 未知
公开(公告)号：DE112005003358T5
公开(公告)日：2008-03-13
申请号：DE112005003358
申请日：2005-09-15
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , WOO HAN MIN , CHOI HYUNG SEOK
IPC分类号： C12Q1/68
摘要： Provided is a method for improving useful substance-producing organisms using metabolic flux analysis, and more particularly a method for improving a host organism producing a useful substance, the method including: calculating a maximum flux value corresponding to the theoretical maximum yield of the useful substance in the metabolic network model of the host organism for producing useful substance, and calculating the optimum value of metabolic flux associated with useful substance production in the metabolic network when the value of cell growth-associated metabolic flux is the maximum under the condition where fermentation data are applied or not applied; selecting metabolic fluxes whose absolute values increase from the range between the maximum value and the optimum value; screening genes associated with the selected metabolic fluxes; and introducing and/or amplifying the selected genes in the host organism. Production of the useful substance can be effectively improved by using the method.

96. 发明专利

AU2007277593A1 Novel engineered microorganism producing homo-succinic acid and method for preparing succinic acid using the same 未知
公开(公告)号：AU2007277593A1
公开(公告)日：2008-01-31
申请号：AU2007277593
申请日：2007-07-25
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , SONG HYOHAK , LIM SUNG WON
IPC分类号： C12N1/21
摘要： The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive mutant microorganism is useful to produce succinic acid for industrial use.

97. 发明专利

FR2869044B1 未知
公开(公告)号：FR2869044B1
公开(公告)日：2006-07-14
申请号：FR0404055
申请日：2004-04-16
申请人： KOREA ADVANCED INST SCI & TECH , SHANGHAI JIATONG UNIVERSITY
发明人： LEE SANG YUP , DENG ZIXING , CHEN SHI , JEONG KI JUN , ZHOU XIUFEN
IPC分类号： C12N15/52 , C07D313/00 , C07D325/00 , C07H17/08 , C07K14/36 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/16 , C12N9/88 , C12N15/31 , C12P17/02 , C12P17/08 , C12P17/18 , C12P19/62 , C12R1/465

98. 发明专利

AU2004292642A1 Novel rumen bacteria variants and process for preparing succinic acid employing the same 未知
公开(公告)号：AU2004292642A1
公开(公告)日：2005-06-09
申请号：AU2004292642
申请日：2004-05-20
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , LEE SANG JUN
IPC分类号： C12N1/21 , C12N1/20 , C12P7/46 , C12R1/01
摘要： Provided are novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene (ldhA) and a pyruvate formate-lyase gene (pfl) from rumen bacteria; a novel bacterial mutant (Mannheimia sp. LPK7) having disruptions of a ldhA, a pfl, a phosphotransacetylase gene (pta), and a acetate kinase gene (ackA); a novel bacterial mutant (Mannheimia sp. LPK4) having disruptions of a ldhA, a pfl, and a phosphoenolpyruvate carboxylase gene (ppc) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, characterized by culture of the above mutants in anaerobic conditions. The bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the bacterial mutants are useful as strains for the industrial production of succinic acid.

99. 发明专利

AU2003284750A1 COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS (sHSPs) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE sHSPs 未知
公开(公告)号：AU2003284750A1
公开(公告)日：2005-03-29
申请号：AU2003284750
申请日：2003-11-24
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG , PARK SI JAE
IPC分类号： G01N33/483 , C07K14/00 , C07K14/195 , C07K14/245 , C12N15/09 , G01N27/447 , G01N33/68
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional (2-D) gel electrophoresis. Furthermore, the present invention relates to the improved method of 2-D gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the 2-D gel electrophoresis, thereby obtaining 2-D gel with much more protein spots.

100. 发明专利

AU2003247177A1 METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYINGibpA AND/OR ibpB GENE CODING FOR INCLUSION BODY-ASSOCIATED PROTEINS 未知
公开(公告)号：AU2003247177A1
公开(公告)日：2004-09-30
申请号：AU2003247177
申请日：2003-07-10
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , HAN MEE JUNG , PARK SI JAE
IPC分类号： C07K14/245 , C07K14/54 , C07K14/57 , C07K14/65 , C12N1/21 , C12N15/70 , C12P21/02
摘要： A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described. The first method enhances the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method enhances the production of target proteins in the cytoplasm and also converts the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式