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    • 99. 发明授权
    • Glucose isomerase mutants, DNA thereof and use thereof
    • 葡萄糖异构酶突变体,其DNA及其用途
    • US07704719B2
    • 2010-04-27
    • US12093859
    • 2006-10-30
    • Jun WangCaike JinRongzhao FuDong Shen
    • Jun WangCaike JinRongzhao FuDong Shen
    • C12N9/00C12N9/92C12N1/20C12N15/00C07H21/04
    • C12N9/92C12P19/24
    • This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.
    • 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gln),并携带至少一种另外的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(wt)或更高浓度果糖的高果糖玉米糖浆。
    • 100. 发明申请
    • Systems and Methods for Dynamic Creation and Release of Proxy Mobile IP Connections
    • 动态创建和释放代理移动IP连接的系统和方法
    • US20100095007A1
    • 2010-04-15
    • US12561828
    • 2009-09-17
    • George CherianJun Wang
    • George CherianJun Wang
    • G06F15/16
    • H04W60/005H04W8/087H04W80/045H04W88/182
    • Disclosed are systems, methods and computer program products for facilitating dynamic addition and release of IP addresses in an established Proxy Mobile IPv6 (PMIP) sessions, for example, based on the demand of a mobile device. An example method comprises receiving from the mobile device a request to add a second IP interface type to a current communication session associated with a first IP interface type, wherein the second IP interface type is different from the first IP interface type. The method further includes receiving from the home network a set of IP addresses of the second IP interface type and establishing one or more IP flows of the second IP interface type using one or more received IP addresses. The method further includes associating the one or more IP flows of the second IP interface type with the current communication session for the mobile device.
    • 公开了用于在已建立的代理移动IPv6(PMIP)会话中动态添加和释放IP地址的系统,方法和计算机程序产品,例如基于移动设备的需求。 一种示例性方法包括从移动设备接收将第二IP接口类型添加到与第一IP接口类型相关联的当前通信会话的请求,其中第二IP接口类型不同于第一IP接口类型。 该方法还包括从归属网络接收第二IP接口类型的一组IP地址,并使用一个或多个接收的IP地址建立第二IP接口类型的一个或多个IP流。 该方法还包括将第二IP接口类型的一个或多个IP流与移动设备的当前通信会话相关联。