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91. 发明申请

US20220221405A1 APPARATUS AND METHOD FOR TRANSFERRING AND ANALYZING SUSPENDED PARTICLES IN A LIQUID SAMPLE 有权
公开(公告)号：US20220221405A1
公开(公告)日：2022-07-14
申请号：US17612242
申请日：2020-05-26
申请人： Robert A. Levine , Stephen C. Wardlaw
发明人： Robert A. Levine , Stephen C. Wardlaw
IPC分类号： G01N21/64 , G01N1/10 , B01L3/00
摘要： The present invention relates to an apparatus, i.e. a device, for transferring a representative sample of a liquid containing particles into a chamber for analysis, such as for example, whole anticoagulated blood. The apparatus comprises an ante-chamber configured for receiving, containing, and transferring a portion of the sample to an analysis chamber. The chambers are continuous with each other, configured to allow the capillary flow of substantially the entirety of the contents of the ante-chamber into the analysis chamber to under-fill or substantially fill the analysis chamber. The apparatus can be conveniently configured as a disposable, single-use device, such as a dipstick, for performing both sampling and analysis from a single apparatus. The apparatus is useful for providing reliable and reproducible blood and body fluid analyses.

92. 发明授权

US09395365B2 Detection of infectious disease in a human or animal by measuring specific phagocytosis in a thin film sample of their anticoagulated blood 有权
标题翻译：通过测量其抗凝血液的薄膜样品中的特异性吞噬作用来检测人或动物中的传染病
公开(公告)号：US09395365B2
公开(公告)日：2016-07-19
申请号：US12417296
申请日：2009-04-02
申请人： Robert A. Levine , Stephen C. Wardlaw
发明人： Robert A. Levine , Stephen C. Wardlaw
IPC分类号： C12Q1/00 , C12Q1/68 , G01N33/53 , G01N30/00 , G01N33/569 , C12Q1/04 , G01N33/50 , G01N33/543 , G01N15/14 , G01N15/00
CPC分类号： G01N33/56911 , C12Q1/04 , G01N15/1475 , G01N33/5094 , G01N33/5432 , G01N2015/008 , Y02A50/57
摘要： A method for performing a target analyte assay of a thin film anticoagulated blood sample, wherein the target analyte is the presence or absence of specific phagocytosis and/or binding of particles coated with a particular antigen or antigens by white blood cells present in the anticoagulated blood sample, wherein the particles are coated with the particular antigen or antigens, which antigens are similar or identical to antigens expressed by a defined pathogenic infectious agent of interest.
摘要翻译：一种用于进行薄膜抗凝血液样品的目标分析物测定的方法，其中所述目标分析物是存在或不存在特定的吞噬作用和/或通过存在于抗凝血液中的白细胞与特定抗原或抗原结合的颗粒的结合样品，其中所述颗粒用特定的抗原或抗原包被，所述抗原与由感兴趣的确定的致病性感染性试剂表达的抗原相似或相同。

93. 发明授权

US09291617B2 Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching 有权
公开(公告)号：US09291617B2
公开(公告)日：2016-03-22
申请号：US14673294
申请日：2015-03-30
申请人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N33/48 , G01N33/50 , G01N21/64
CPC分类号： G01N33/49 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N33/48 , G01N33/5091 , G01N2015/008 , G01N2015/1477 , G01N2021/6432 , G01N2021/6439 , G01N2201/062
摘要： A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.

94. 发明授权

US08481282B2 Method for detecting the presence of anisotropic crystals in undiluted whole blood 失效
标题翻译：检测未稀释全血中各向异性晶体存在的方法
公开(公告)号：US08481282B2
公开(公告)日：2013-07-09
申请号：US13190242
申请日：2011-07-25
申请人： Robert A. Levine , Darryn W. Unfricht , Stephen C. Wardlaw , Benjamin Ports
发明人： Robert A. Levine , Darryn W. Unfricht , Stephen C. Wardlaw , Benjamin Ports
IPC分类号： C12Q1/04
CPC分类号： G01N21/23 , G01N21/6458
摘要： An apparatus and method for detecting the presence of anisotropic crystals within a biologic fluid sample is provided. The method includes the steps of: a) disposing the sample within a sample chamber in a sample layer having a height not greater than about fifteen microns (15μ); b) disposing the sample layer within the sample chamber between a polarizing filter and an analyzing filter; c) disposing the polarizing filter, sample chamber, and analyzing filter in a configuration relative to a light source such that polarized light passes through the sample, and subsequently impinges on the analyzing filter; and d) wherein polar orientations of the polarizing filter and the analyzing filter are such that the polarized light will not pass through the analyzing filter, and light passing through an anisotropic crystal disposed within the sample will pass through the analyzing filter and appear as a point of light.
摘要翻译：提供了一种用于检测生物液体样品中各向异性晶体的存在的装置和方法。该方法包括以下步骤：a）将样品置于具有不大于约15微米（15mu）的高度的样品层中的样品室内; b）将样品层置于样品室内，在偏振滤光片和分析滤光片之间; c）将偏振滤光器，样品室和分析滤光片相对于光源配置成使得偏振光通过样品，随后撞击在分析滤光片上; 和d）其中偏振滤光器和分析滤光器的极性取向使得偏振光不会通过分析滤光器，并且穿过设置在样品内的各向异性晶体的光将通过分析滤光器并显现为点的光。

95. 发明授权

US08472693B2 Method for determining at least one hemoglobin related parameter of a whole blood sample 有权
标题翻译：用于确定全血样品的至少一种血红蛋白相关参数的方法
公开(公告)号：US08472693B2
公开(公告)日：2013-06-25
申请号：US13051705
申请日：2011-03-18
申请人： Graham Davis , Stephen C. Wardlaw , Robert A. Levine , Darryn W. Unfricht , Niten Lalpuria , Paul G. Mitsis
发明人： Graham Davis , Stephen C. Wardlaw , Robert A. Levine , Darryn W. Unfricht , Niten Lalpuria , Paul G. Mitsis
IPC分类号： G06K9/00
CPC分类号： G01N33/726 , G01N15/1475 , G01N2015/0073
摘要： A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.
摘要翻译：提供了一种用于确定全血样品的至少一种血红蛋白相关参数的方法和装置。该方法包括以下步骤：a）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板的内表面和第二面板的内表面限定，并且所述室具有在所述面板的内表面之间延伸的高度，其中所述腔被配置为在进入所述腔室之后的预定量的时间内将所述样品的氧合状态增加到基本上氧合状态; b）对与内表面接触的至少一个红细胞进行成像，并产生图像信号; c）确定接触两个内表面的成像红细胞的至少一部分的光密度; 以及d）使用确定的光密度和氧合血红蛋白的摩尔消光系数来确定与内表面接触的红细胞的至少一种血红蛋白相关参数。

96. 发明授权

US08310659B2 Method and apparatus for detecting and counting platelets individually and in aggregate clumps 有权
标题翻译：用于单独和聚集团中血小板检测和计数的方法和装置
公开(公告)号：US08310659B2
公开(公告)日：2012-11-13
申请号：US13323438
申请日：2011-12-12
申请人： Stephen C. Wardlaw , Robert A. Levine , Niten V. Lalpuria
发明人： Stephen C. Wardlaw , Robert A. Levine , Niten V. Lalpuria
IPC分类号： G01N33/48
CPC分类号： G01N33/49 , G01N15/147 , G01N15/1475 , G01N2015/0076 , G01N2015/008 , G01N2015/0084 , G01N2015/0092 , G01N2015/1486
摘要： A method for enumerating platelets within a blood sample is provided. The method includes the steps of: 1) depositing the sample into a sample container having an analysis chamber adapted to quiescently hold the sample for analysis, and an amount of colorant that platelets absorb and which fluoresces upon exposure to one or more predetermined first wavelengths of light; 2) imaging at least a portion of the sample disposed in the analysis chamber, including producing image signals indicative of fluorescent emissions from the platelets illuminated by first wavelengths of light; 3) identifying the platelets using the image signals; and 4) enumerating individual platelets and clumped platelets within the sample using one or more of fluorescent emissions, area, shape, and granularity.
摘要翻译：提供了一种用于列举血液样品中的血小板的方法。该方法包括以下步骤：1）将样品沉积到具有分析室的样品容器中，所述分析室适于静态保持样品进行分析，以及一定量的着色剂，血小板被吸收并且在暴露于一个或多个预定的第一波长光; 2）对设置在分析室中的样品的至少一部分进行成像，包括产生指示由第一波长的光照射的血小板的荧光发射的图像信号; 3）使用图像信号识别血小板; 和4）使用荧光发射，面积，形状和粒度中的一种或多种来列举样品中的各个血小板和聚集的血小板。

97. 发明申请

US20120164682A1 METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING 有权
标题翻译：使用荧光猝灭和/或漂白分析个体细胞或颗粒物的方法和装置
公开(公告)号：US20120164682A1
公开(公告)日：2012-06-28
申请号：US13330253
申请日：2011-12-19
申请人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N21/64
CPC分类号： G01N33/49 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N33/48 , G01N33/5091 , G01N2015/008 , G01N2015/1477 , G01N2021/6432 , G01N2021/6439 , G01N2201/062
摘要： A method for analyzing a blood sample residing within an analysis chamber, which sample has one or more first and second constituents, which constituents are different from one another. The method includes the steps of: a) imaging the sample containing the first and second constituents, which sample is mixed with a colorant that causes the first and second constituents to fluoresce upon exposure to light, and which colorant is also operative to absorb light, the imaging produces image signals representative of fluorescent emissions from the sample and optical density of the sample; b) determining one or more values representative of fluorescent emission from the first and second constituents; c) determining one or more values representative of optical density for the first and second constituents; and d) distinguishing the first constituents from the second constituents using the fluorescent emission representative values and the optical density representative values.
摘要翻译：一种用于分析驻留在分析室内的血液样本的方法，该样品具有一个或多个第一和第二组分，该组分彼此不同。该方法包括以下步骤：a）对包含第一和第二成分的样品进行成像，该样品与着色剂混合，使得第一和第二组分在暴露于光时发荧光，并且哪种着色剂也可操作地吸收光，成像产生代表样品的荧光发射和样品的光密度的图像信号; b）确定代表来自第一和第二组分的荧光发射的一个或多个值; c）确定表示第一和第二组分的光密度的一个或多个值; 以及d）使用荧光发射代表值和光密度代表值来区分第一组分与第二组分。

98. 发明申请

US20110294200A1 VIRTUAL SEPARATION OF BOUND AND FREE LABEL IN A LIGAND ASSAY FOR PERFORMING IMMUNOASSAYS OF BIOLOGICAL FLUIDS, INCLUDING WHOLE BLOOD 有权
标题翻译：用于执行生物流体免疫测定的配体测定中的粘合剂和免费标签的虚拟分离，包括全血
公开(公告)号：US20110294200A1
公开(公告)日：2011-12-01
申请号：US13204416
申请日：2011-08-05
申请人： Stephen C. Wardlaw , Robert A. Levine
发明人： Stephen C. Wardlaw , Robert A. Levine
IPC分类号： C12M1/34 , G01N33/53
CPC分类号： G01N33/48 , G01N21/6428 , G01N33/5304 , G01N33/54373 , G01N33/54386
摘要： Detection and characterization of immunologically detected substances are performed electronically on human and animal biological fluids such as whole blood, serum, plasma, urine, milk, pleural and peritoneal fluids, and semen, which fluids are contained in a thin chamber forming a quiescent fluid sample, which chamber has at least two parallel planar walls, at least one of which is transparent.
摘要翻译：免疫检测物质的检测和表征以人和动物生物流体如全血，血清，血浆，尿，牛奶，胸膜和腹膜液以及精液电子方式进行，这些流体包含在形成静态流体样品的薄室中该室具有至少两个平行的平面壁，其中至少一个是透明的。

99. 发明授权

US07995194B2 Virtual separation of bound and free label in a ligand assay for performing immunoassays of biological fluids, including whole blood 有权
标题翻译：用于进行生物液体包括全血的免疫测定的配体测定中的结合和游离标记的虚拟分离
公开(公告)号：US07995194B2
公开(公告)日：2011-08-09
申请号：US12417246
申请日：2009-04-02
申请人： Stephen C. Wardlaw , Robert A. Levine
发明人： Stephen C. Wardlaw , Robert A. Levine
IPC分类号： G01N33/48 , G01N21/03
CPC分类号： G01N33/48 , G01N21/6428 , G01N33/5304 , G01N33/54373 , G01N33/54386
摘要： Detection and characterization of immunologically detected substances are performed electronically on human and animal biological fluids such as whole blood, serum, plasma, urine, milk, pleural and peritoneal fluids, and semen, which fluids are contained in a thin chamber forming a quiescent fluid sample, which chamber has at least two parallel planar walls, at least one of which is transparent.
摘要翻译：免疫检测物质的检测和表征以人和动物生物流体如全血，血清，血浆，尿，牛奶，胸膜和腹膜液以及精液电子方式进行，这些流体包含在形成静态流体样品的薄室中该室具有至少两个平行的平面壁，其中至少一个是透明的。

100. 发明授权

US07929121B2 Method and apparatus for detecting and counting platelets individually and in aggregate clumps 有权
标题翻译：用于单独和聚集团中血小板检测和计数的方法和装置
公开(公告)号：US07929121B2
公开(公告)日：2011-04-19
申请号：US12408500
申请日：2009-03-20
申请人： Stephen C. Wardlaw , Robert A. Levine , Niten V. Lalpuria
发明人： Stephen C. Wardlaw , Robert A. Levine , Niten V. Lalpuria
IPC分类号： G01N33/48
CPC分类号： G01N33/49 , G01N15/147 , G01N15/1475 , G01N2015/0076 , G01N2015/008 , G01N2015/0084 , G01N2015/0092 , G01N2015/1486
摘要： A method for enumerating platelets within a blood sample is provided. The method includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; 2) admixing a colorant with the sample, which colorant is operative to cause the platelets to fluoresce upon exposure to one or more predetermined first wavelengths of light; 3) illuminating at least a portion of the sample containing the platelets at the first wavelengths; 4) imaging the sample, including producing image signals indicative of fluorescent emissions from the platelets, which fluorescent emissions have an intensity; 5) identifying the platelets by their fluorescent emissions, using the image signals; 6) determining an average fluorescent emission intensity value for the individual platelets identified within the sample; 7) identifying clumps of platelets within the sample using one or more of their fluorescent emissions, area, shape, and granularity; and 8) enumerating platelets within each platelet clump using the average fluorescent emission intensity value determined for the individual platelets within the sample.
摘要翻译：提供了一种用于列举血液样品中的血小板的方法。该方法包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板和第二面板限定，两个面板都是透明的; 2）将着色剂与所述样品混合，所述着色剂在暴露于一个或多个预定的第一波长的光时会使血小板发荧光; 3）以第一波长照射含有血小板的样品的至少一部分; 4）对样品进行成像，包括产生指示来自血小板的荧光发射的图像信号，该荧光发射具有强度; 5）使用图像信号通过荧光发射识别血小板; 6）确定样品内识别的各个血小板的平均荧光发射强度值; 7）使用其荧光发射，面积，形状和粒度中的一种或多种来鉴定样品中的血小板团块; 和8）使用为样品中的各个血小板确定的平均荧光发射强度值来列举每个血小板聚集体内的血小板。

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